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Literature DB >> 3681984

At least six nucleotides preceding the AUG initiator codon enhance translation in mammalian cells.

Abstract

Sequences flanking the AUG initiator codon influence its recognition by eukaryotic ribosomes. From a comparison of several hundred mRNA sequences, CCA/GCCAUGG emerged as the consensus sequence for initiation in higher eukaryotes. Systematic mutagenesis of a cloned preproinsulin gene confirmed the facilitating effect of A or G in position -3 (i.e. 3 nucleotides upstream from the AUG codon), C in positions -1 and -2, and G immediately following the AUG codon. The analysis of a new set of mutants now reveals that sequences slightly farther upstream are also influential, the optimal context for initiation being (GCC)GCCA/GCCAUGG. Possible mechanistic implications of the repeating GCC motif are discussed.

Entities: Gene Species

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Year: 1987 PMID： 3681984 DOI： 10.1016/0022-2836(87)90418-9

Source DB: PubMed Journal: J Mol Biol ISSN： 0022-2836 Impact factor: 5.469

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Cited

350 in total

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Authors: S M Nielsen; L Z Nielsen; S A Hjorth; M H Perrin; W W Vale
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2. Transcriptional regulation of the CLC-K1 promoter by myc-associated zinc finger protein and kidney-enriched Krüppel-like factor, a novel zinc finger repressor.

Authors: S Uchida; Y Tanaka; H Ito; F Saitoh-Ohara; J Inazawa; K K Yokoyama; S Sasaki; F Marumo
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3. A mutation in the short 5'-proximal open reading frame on Rous sarcoma virus RNA alters virus production.

Authors: R B Petersen; A Moustakas; P B Hackett
Journal: J Virol Date: 1989-11 Impact factor: 5.103

4. High-efficiency incorporation of functional influenza virus glycoproteins into recombinant vesicular stomatitis viruses.

Authors: E Kretzschmar; L Buonocore; M J Schnell; J K Rose
Journal: J Virol Date: 1997-08 Impact factor: 5.103

10. Mouse embryonic stem cells have underdeveloped antiviral mechanisms that can be exploited for the development of mRNA-mediated gene expression strategy.

Authors: Ruoxing Wang; Chengwen Teng; Joseph Spangler; Jundi Wang; Faqing Huang; Yan-Lin Guo
Journal: Stem Cells Dev Date: 2013-12-20 Impact factor: 3.272

At least six nucleotides preceding the AUG initiator codon enhance translation in mammalian cells.

1. Constitutive activation of tethered-peptide/corticotropin-releasing factor receptor chimeras.

2. Transcriptional regulation of the CLC-K1 promoter by myc-associated zinc finger protein and kidney-enriched Krüppel-like factor, a novel zinc finger repressor.

3. A mutation in the short 5'-proximal open reading frame on Rous sarcoma virus RNA alters virus production.

4. High-efficiency incorporation of functional influenza virus glycoproteins into recombinant vesicular stomatitis viruses.

5. Iron-dependent self-assembly of recombinant yeast frataxin: implications for Friedreich ataxia.

6. Mouse VAP33 is associated with the endoplasmic reticulum and microtubules.

7. The splicing of U12-type introns can be a rate-limiting step in gene expression.

8. PLAIDD, a type II death domain protein that interacts with p75 neurotrophin receptor.

9. Selective inhibition of angiotensin receptor signaling through Erk1/2 pathway by a novel peptide.

10. Mouse embryonic stem cells have underdeveloped antiviral mechanisms that can be exploited for the development of mRNA-mediated gene expression strategy.