Literature DB >> 9371336

Characterization of a new TEM-derived beta-lactamase produced in a Serratia marcescens strain.

M Perilli1, A Felici, N Franceschini, A De Santis, L Pagani, F Luzzaro, A Oratore, G M Rossolini, J R Knox, G Amicosante.   

Abstract

A natural TEM variant beta-lactamase was isolated from an epidemic strain of Serratia marcescens. Nucleotide gene sequencing revealed multiple point mutations located in the 42-to-44 tripeptide and positions 145 to 146, 178, and 238. In addition, a glutamic acid 212 deletion was also found. The purified enzyme was studied from a kinetic point of view, revealing the highest catalytic efficiency (k[cat]/Km) values for ceftazidime and aztreonam compared with the TEM-1 prototype enzyme. The in vitro resistance correlated with kinetic parameters, and the enzyme also mediated resistance to some penicillins and an ampicillin-clavulanic acid combination. The mutational and kinetic changes are discussed in relation to the three-dimensional crystallographic structure of the wild-type TEM-1 enzyme.

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Year:  1997        PMID： 9371336      PMCID： PMC164131     

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  37 in total

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Journal:  Antimicrob Agents Chemother       Date:  1989-03       Impact factor: 5.191

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Journal:  Antimicrob Agents Chemother       Date:  1991-09       Impact factor: 5.191

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Journal:  Biochem J       Date:  1992-06-01       Impact factor: 3.857

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6.  A new TEM beta-lactamase double mutant with broadened specificity reveals substrate-dependent functional interactions.

Authors:  H Viadiu; J Osuna; A L Fink; X Soberón
Journal:  J Biol Chem       Date:  1995-01-13       Impact factor: 5.157

7.  Evolution of antibiotic resistance: several different amino acid substitutions in an active site loop alter the substrate profile of beta-lactamase.

Authors:  T Palzkill; Q Q Le; K V Venkatachalam; M LaRocco; H Ocera
Journal:  Mol Microbiol       Date:  1994-04       Impact factor: 3.501

8.  Complementary roles of mutations at positions 69 and 242 in a class A beta-lactamase.

Authors:  R A Bonomo; C G Dawes; J R Knox; D M Shlaes
Journal:  Biochim Biophys Acta       Date:  1995-02-22

9.  Mechanism of acyl transfer by the class A serine beta-lactamase of Streptomyces albus G.

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Journal:  Biochem J       Date:  1991-10-01       Impact factor: 3.857

10.  The crystal structure of the beta-lactamase of Streptomyces albus G at 0.3 nm resolution.

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Journal:  Biochem J       Date:  1987-08-01       Impact factor: 3.857
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  16 in total

1.  Molecular characterization of extended-spectrum beta-lactamases produced by nosocomial isolates of Enterobacteriaceae from an Italian nationwide survey.

Authors:  Mariagrazia Perilli; Emanuela Dell'Amico; Bernardetta Segatore; Maria Rosaria de Massis; Ciro Bianchi; Francesco Luzzaro; Gian Maria Rossolini; Antonio Toniolo; Giuseppe Nicoletti; Gianfranco Amicosante
Journal:  J Clin Microbiol       Date:  2002-02       Impact factor: 5.948

Review 2.  Extended-spectrum beta-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat.

Authors:  P A Bradford
Journal:  Clin Microbiol Rev       Date:  2001-10       Impact factor: 26.132

3.  Biochemical characterization of TEM-92 extended-spectrum beta-lactamase, a protein differing from TEM-52 in the signal peptide.

Authors:  Mariagrazia Perilli; Bernardetta Segatore; Maria Rosaria De Massis; Laura Pagani; Francesco Luzzaro; Gian Maria Rossolini; Gianfranco Amicosante
Journal:  Antimicrob Agents Chemother       Date:  2002-12       Impact factor: 5.191

4.  Evaluation of four commercially available extended-spectrum beta-lactamase phenotypic confirmation tests.

Authors:  Andrea J Linscott; William J Brown
Journal:  J Clin Microbiol       Date:  2005-03       Impact factor: 5.948

Review 5.  Beta-lactamase nomenclature.

Authors:  George A Jacoby
Journal:  Antimicrob Agents Chemother       Date:  2006-04       Impact factor: 5.191

6.  Identification and characterization of a new metallo-beta-lactamase, IND-5, from a clinical isolate of Chryseobacterium indologenes.

Authors:  Mariagrazia Perilli; Bibiana Caporale; Giuseppe Celenza; Cristina Pellegrini; Jean Denis Docquier; Marilina Mezzatesta; Gian Maria Rossolini; Stefania Stefani; Gianfranco Amicosante
Journal:  Antimicrob Agents Chemother       Date:  2007-04-30       Impact factor: 5.191

7.  Repeated epidemics caused by extended-spectrum beta-lactamase-producing Serratia marcescens strains.

Authors:  F Luzzaro; M Perilli; R Migliavacca; G Lombardi; P Micheletti; A Agodi; S Stefani; G Amicosante; L Pagani
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1998-09       Impact factor: 3.267

8.  Novel beta-lactamase genes from two environmental isolates of Vibrio harveyi.

Authors:  J W Teo; A Suwanto; C L Poh
Journal:  Antimicrob Agents Chemother       Date:  2000-05       Impact factor: 5.191

9.  Characterization of the extended-spectrum beta-lactamase reference strain, Klebsiella pneumoniae K6 (ATCC 700603), which produces the novel enzyme SHV-18.

Authors:  J K Rasheed; G J Anderson; H Yigit; A M Queenan; A Doménech-Sánchez; J M Swenson; J W Biddle; M J Ferraro; G A Jacoby; F C Tenover
Journal:  Antimicrob Agents Chemother       Date:  2000-09       Impact factor: 5.191

10.  TEM-72, a new extended-spectrum beta-lactamase detected in Proteus mirabilis and Morganella morganii in Italy.

Authors:  M Perilli; B Segatore; M R de Massis; M L Riccio; C Bianchi; A Zollo; G M Rossolini; G Amicosante
Journal:  Antimicrob Agents Chemother       Date:  2000-09       Impact factor: 5.191
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