Literature DB >> 12435705

Biochemical characterization of TEM-92 extended-spectrum beta-lactamase, a protein differing from TEM-52 in the signal peptide.

Mariagrazia Perilli1, Bernardetta Segatore, Maria Rosaria De Massis, Laura Pagani, Francesco Luzzaro, Gian Maria Rossolini, Gianfranco Amicosante.   

Abstract

A bla(TEM-92) gene was cloned from a Proteus mirabilis isolate and expressed in Escherichia coli. Production of the enzyme caused reduction of susceptibility to penicillins and narrow- to expanded-spectrum cephalosporins but not to moxalactam and cephamycins. Determination of kinetic parameters with the purified enzyme revealed hydrolysis of expanded-spectrum cephalosporins, while cephamycins, moxalactam, and aztreonam were very poorly or not hydrolyzed. Clavulanate and penicillanic acid sulfones acylated TEM-92 slowly, and deacylation occurred at measurable rates.

Entities:  

Mesh：

Substances：

Year:  2002        PMID： 12435705      PMCID： PMC132775          DOI： 10.1128/AAC.46.12.3981-3983.2002

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  15 in total

1.  New TEM variant (TEM-92) produced by Proteus mirabilis and Providencia stuartii isolates.

Authors:  C de Champs; C Monne; R Bonnet; W Sougakoff; D Sirot; C Chanal; J Sirot
Journal:  Antimicrob Agents Chemother       Date:  2001-04       Impact factor: 5.191

2.  Extended-spectrum TEM- and SHV-type beta-lactamase-producing Klebsiella pneumoniae strains causing outbreaks in intensive care units in Italy.

Authors:  L Pagani; M Perilli; R Migliavacca; F Luzzaro; G Amicosante
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2000-10       Impact factor: 3.267

3.  Molecular characterization of extended-spectrum beta-lactamases produced by nosocomial isolates of Enterobacteriaceae from an Italian nationwide survey.

Authors:  Mariagrazia Perilli; Emanuela Dell'Amico; Bernardetta Segatore; Maria Rosaria de Massis; Ciro Bianchi; Francesco Luzzaro; Gian Maria Rossolini; Antonio Toniolo; Giuseppe Nicoletti; Gianfranco Amicosante
Journal:  J Clin Microbiol       Date:  2002-02       Impact factor: 5.948

Review 4.  Extended-spectrum beta-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat.

Authors:  P A Bradford
Journal:  Clin Microbiol Rev       Date:  2001-10       Impact factor: 26.132

5.  Automated analysis of enzyme inactivation phenomena. Application to beta-lactamases and DD-peptidases.

Authors:  F De Meester; B Joris; G Reckinger; C Bellefroid-Bourguignon; J M Frère; S G Waley
Journal:  Biochem Pharmacol       Date:  1987-07-15       Impact factor: 5.858

6.  Interactions between active-site-serine beta-lactamases and compounds bearing a methoxy side chain on the alpha-face of the beta-lactam ring: kinetic and molecular modelling studies.

Authors:  A Matagne; J Lamotte-Brasseur; G Dive; J R Knox; J M Frère
Journal:  Biochem J       Date:  1993-08-01       Impact factor: 3.857

7.  TEM beta-lactamase mutants hydrolysing third-generation cephalosporins. A kinetic and molecular modelling analysis.

Authors:  X Raquet; J Lamotte-Brasseur; E Fonzé; S Goussard; P Courvalin; J M Frère
Journal:  J Mol Biol       Date:  1994-12-16       Impact factor: 5.469

8.  Survey of extended-spectrum beta-lactamases in clinical isolates of Escherichia coli and Klebsiella pneumoniae: prevalence of TEM-52 in Korea.

Authors:  H Pai; S Lyu; J H Lee; J Kim; Y Kwon; J W Kim; K W Choe
Journal:  J Clin Microbiol       Date:  1999-06       Impact factor: 5.948

9.  Characterization of a new extended-spectrum beta-lactamase (TEM-87) isolated in Proteus mirabilis during an Italian survey.

Authors:  Mariagrazia Perilli; Bernardetta Segatore; Maria Rosaria De Massis; Nicola Franceschini; Ciro Bianchi; Gian Maria Rossolini; Gianfranco Amicosante
Journal:  Antimicrob Agents Chemother       Date:  2002-03       Impact factor: 5.191

10.  Role of positive charge on the amino-terminal region of the signal peptide in protein secretion across the membrane.

Authors:  S Inouye; X Soberon; T Franceschini; K Nakamura; K Itakura; M Inouye
Journal:  Proc Natl Acad Sci U S A       Date:  1982-06       Impact factor: 11.205
View more
  1 in total

1.  Proteus mirabilis bloodstream infections: risk factors and treatment outcome related to the expression of extended-spectrum beta-lactamases.

Authors:  Andrea Endimiani; Francesco Luzzaro; Gioconda Brigante; Mariagrazia Perilli; Gianluigi Lombardi; Gianfranco Amicosante; Gian Maria Rossolini; Antonio Toniolo
Journal:  Antimicrob Agents Chemother       Date:  2005-07       Impact factor: 5.191
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.