Literature DB >> 9367812

Purification of ribonucleases Sa, Sa2, and Sa3 after expression in Escherichia coli.

E J Hebert1, G R Grimsley, R W Hartley, G Horn, D Schell, S Garcia, V Both, J Sevcik, C N Pace.   

Abstract

The genes for three small ribonucleases from different strains of Streptomyces aureofaciens have been cloned and expressed in Escherichia coli. The purification of these ribonucleases from the periplasmic space is described. The yields range from 10 to 50 mg of protein per liter of culture medium. The molar absorption coefficients, isoelectric pH values, and pH of optimum activity are reported. Copyright 1997 Academic Press.

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Year:  1997        PMID： 9367812     DOI： 10.1006/prep.1997.0776

Source DB:  PubMed          Journal:  Protein Expr Purif        ISSN： 1046-5928            Impact factor:   1.650


  20 in total

1.  Increasing protein stability by altering long-range coulombic interactions.

Authors:  G R Grimsley; K L Shaw; L R Fee; R W Alston; B M Huyghues-Despointes; R L Thurlkill; J M Scholtz; C N Pace
Journal:  Protein Sci       Date:  1999-09       Impact factor: 6.725

2.  Sequential assignment and solution secondary structure of doubly labelled ribonuclease Sa.

Authors:  D V Laurents; J M Pérez-Cañadillas; J Santoro; M Rico; D Schell; E J Hebert; C N Pace; M Bruix
Journal:  J Biomol NMR       Date:  1999-05       Impact factor: 2.835

3.  The effect of net charge on the solubility, activity, and stability of ribonuclease Sa.

Authors:  K L Shaw; G R Grimsley; G I Yakovlev; A A Makarov; C N Pace
Journal:  Protein Sci       Date:  2001-06       Impact factor: 6.725

4.  Changing the net charge from negative to positive makes ribonuclease Sa cytotoxic.

Authors:  Olga N Ilinskaya; Florian Dreyer; Vladimir A Mitkevich; Kevin L Shaw; C Nick Pace; Alexander A Makarov
Journal:  Protein Sci       Date:  2002-10       Impact factor: 6.725

5.  Contribution of active site residues to the activity and thermal stability of ribonuclease Sa.

Authors:  Gennady I Yakovlev; Vladimir A Mitkevich; Kevin L Shaw; Saul Trevino; Stephanie Newsom; C Nick Pace; Alexander A Makarov
Journal:  Protein Sci       Date:  2003-10       Impact factor: 6.725

6.  Toward a molecular understanding of protein solubility: increased negative surface charge correlates with increased solubility.

Authors:  Ryan M Kramer; Varad R Shende; Nicole Motl; C Nick Pace; J Martin Scholtz
Journal:  Biophys J       Date:  2012-04-18       Impact factor: 4.033

7.  Increasing protein stability: importance of DeltaC(p) and the denatured state.

Authors:  Hailong Fu; Gerald Grimsley; J Martin Scholtz; C Nick Pace
Journal:  Protein Sci       Date:  2010-05       Impact factor: 6.725

8.  Charge-charge interactions in the denatured state influence the folding kinetics of ribonuclease Sa.

Authors:  Jared M Trefethen; C Nick Pace; J Martin Scholtz; David N Brems
Journal:  Protein Sci       Date:  2005-06-03       Impact factor: 6.725

9.  Increasing protein conformational stability by optimizing beta-turn sequence.

Authors:  Saul R Trevino; Stephanie Schaefer; J Martin Scholtz; C Nick Pace
Journal:  J Mol Biol       Date:  2007-08-09       Impact factor: 5.469

10.  Increased alkalotolerant and thermostable ribonuclease (RNase) production from alkaliphilic Streptomyces sp. M49-1 by optimizing the growth conditions using response surface methodology.

Authors:  Tuğçe Demir; Özkan Gübe; Mesut Yücel; E Esin Hameş-Kocabaş
Journal:  World J Microbiol Biotechnol       Date:  2013-03-27       Impact factor: 3.312
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