Literature DB >> 9355733

Lysosomal alpha-mannosidases of mouse tissues: characteristics of the isoenzymes, and cloning and expression of a full-length cDNA.

T Beccari1, M G Appolloni, E Costanzi, S Stinchi, J L Stirling, M A Della Fazia, G Servillo, M P Viola, A Orlacchio.   

Abstract

Lysosomal alpha-d-mannosidase from mouse tissues was separated into its constituent isoenzymes by DEAE-cellulose chromatography. Forms corresponding to the human isoenzymes B and A were present in testis, brain, spleen and kidney, whereas in epididymis and liver only the B form was present. Murine alpha-mannosidases A and B are glycoproteins and have pH optima, thermal stabilities and molecular masses similar to those of the human isoenzymes. A full-length cDNA (3.1 kb) containing the complete coding sequence for alpha-mannosidase was isolated from a mouse macrophage cDNA library. Comparison of the deduced amino acid sequences of human and mouse alpha-mannosidases showed that they had 75% identity and 83% similarity. Expression of this cDNA in COS cells showed that both the A and the B isoenzymes can arise from a single transcript. Northern blotting analysis showed a 10-fold range in the abundance of alpha-mannosidase mRNA in mouse tissues, with the highest levels found in epididymis, and the lowest in liver.

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Year:  1997        PMID： 9355733      PMCID： PMC1218761          DOI： 10.1042/bj3270045

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  16 in total

1.  The relationship between different forms of human alpha-mannosidase.

Authors:  M A Chester; A Lundblad; P K Masson
Journal:  Biochim Biophys Acta       Date:  1975-06-24

2.  Blood testosterone levels are correlated with beta-N-acetylhexosaminidase activity in human caput epididymis.

Authors:  A Datti; T Beccari; C Emiliani; J L Stirling; A Orlacchio
Journal:  Biochem Mol Biol Int       Date:  1993-08

3.  Different expression of beta-N-acetylhexosaminidase in mouse tissues.

Authors:  M A Della Fazia; T Beccari; G Servillo; M P Viola-Magni; A Orlacchio
Journal:  Biochem Biophys Res Commun       Date:  1994-03-30       Impact factor: 3.575

4.  Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs.

Authors:  M Kozak
Journal:  Nucleic Acids Res       Date:  1984-01-25       Impact factor: 16.971

5.  Expression of human lysosomal alpha-mannosidase activity in transfected murine cells and human alpha-mannosidase deficient fibroblasts.

Authors:  W Wang; V L Nebes; M C Schmidt; J A Barranger
Journal:  Biochem Biophys Res Commun       Date:  1996-07-25       Impact factor: 3.575

6.  A serological investigation into the acidic alpha-D-mannosidase in normal Angus cattle and in a calf with mannosidosis.

Authors:  N C Phillips; B G Winchester
Journal:  Biochem J       Date:  1977-05-01       Impact factor: 3.857

7.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

8.  Partial sequence of the purified protein confirms the identity of cDNA coding for human lysosomal alpha-mannosidase B.

Authors:  C Emiliani; S Martino; J L Stirling; B Maras; A Orlacchio
Journal:  Biochem J       Date:  1995-01-15       Impact factor: 3.857

9.  Purification and comparison of the structures of human liver acidic alpha-D-mannosidases A and B.

Authors:  S H Cheng; S Malcolm; S Pemble; B Winchester
Journal:  Biochem J       Date:  1986-01-01       Impact factor: 3.857

Review 10.  Mammalian alpha-mannosidases--multiple forms but a common purpose?

Authors:  P F Daniel; B Winchester; C D Warren
Journal:  Glycobiology       Date:  1994-10       Impact factor: 4.313
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  5 in total

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2.  Man2C1, an alpha-mannosidase, is involved in the trimming of free oligosaccharides in the cytosol.

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3.  Spectrum of mutations in alpha-mannosidosis.

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Journal:  Am J Hum Genet       Date:  1999-01       Impact factor: 11.025

Review 4.  Alpha-Mannosidosis: Therapeutic Strategies.

Authors:  Maria Rachele Ceccarini; Michela Codini; Carmela Conte; Federica Patria; Samuela Cataldi; Matteo Bertelli; Elisabetta Albi; Tommaso Beccari
Journal:  Int J Mol Sci       Date:  2018-05-17       Impact factor: 5.923

5.  The Evolution of Leaf Function during Development Is Reflected in Profound Changes in the Metabolic Composition of the Vacuole.

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Journal:  Metabolites       Date:  2021-12-06
  5 in total

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