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Literature DB >> 7832746

Partial sequence of the purified protein confirms the identity of cDNA coding for human lysosomal alpha-mannosidase B.

C Emiliani¹, S Martino, J L Stirling, B Maras, A Orlacchio.

Abstract

Human lysosomal alpha-mannosidase has been purified by a simple and rapid method in sufficient quantities for the analysis of its subunit composition and partial protein sequencing. Analysis of the N-terminal residues of the 30 kDa polypeptide has enabled us to confirm the identity of the recently cloned cDNA that was tentatively identified as that of lysosomal alpha-mannosidase [Nebes and Schmidt (1994) Biochem. Biophys. Res. Commun. 200, 239-245] and to locate the position of this polypeptide within the total deduced amino acid sequence. This finding will therefore provide a firm foundation for the characterization of alpha-mannosidosis mutations.

Entities: Disease Gene Species

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Year: 1995 PMID： 7832746 PMCID： PMC1136369 DOI： 10.1042/bj3050363

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

13 in total

1. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors: M M Bradford
Journal: Anal Biochem Date: 1976-05-07 Impact factor: 3.365

2. Continuous growth and differentiation of human myeloid leukaemic cells in suspension culture.

Authors: S J Collins; R C Gallo; R E Gallagher
Journal: Nature Date: 1977-11-24 Impact factor: 49.962

3. Proteolytic processing of pro-alpha and pro-beta precursors from human beta-hexosaminidase. Generation of the mature alpha and beta a beta b subunits.

Authors: D J Mahuran; K Neote; M H Klavins; A Leung; R A Gravel
Journal: J Biol Chem Date: 1988-04-05 Impact factor: 5.157

2. Lysosomal alpha-mannosidases of mouse tissues: characteristics of the isoenzymes, and cloning and expression of a full-length cDNA.

Authors: T Beccari; M G Appolloni; E Costanzi; S Stinchi; J L Stirling; M A Della Fazia; G Servillo; M P Viola; A Orlacchio
Journal: Biochem J Date: 1997-10-01 Impact factor: 3.857

2 in total

Partial sequence of the purified protein confirms the identity of cDNA coding for human lysosomal alpha-mannosidase B.

1. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

2. Continuous growth and differentiation of human myeloid leukaemic cells in suspension culture.

3. Proteolytic processing of pro-alpha and pro-beta precursors from human beta-hexosaminidase. Generation of the mature alpha and beta a beta b subunits.

4. Human mannosidosis--the enzyme defect.

5. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

6. Synthesis of lysosomal alpha-mannosidase in normal and mannosidosis fibroblasts.

7. Purification and chemical characterization of human acidic alpha-D-mannosidase.

8. Characterization of human liver alpha-D-mannosidase purified by affinity chromatography.

9. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.

10. Purification and comparison of the structures of human liver acidic alpha-D-mannosidases A and B.

1. Assignment of lysosomal alpha-D-mannosidase to mouse chromosome 8.

2. Lysosomal alpha-mannosidases of mouse tissues: characteristics of the isoenzymes, and cloning and expression of a full-length cDNA.