Literature DB >> 9230404

Evaluation of a rapid air thermal cycler for detection of Mycobacterium tuberculosis.

K Chapin1, T L Lauderdale.   

Abstract

The Air Thermal Cycler (ATC) (Idaho Technology, Idaho Falls, Idaho) utilizes the unique technology of small-volume glass capillary tubes and high-velocity air for the heating and cooling medium for the PCR. Standard heat block thermal cycler (HBTC) and ATC performance characteristics were compared for the detection of Mycobacterium tuberculosis. Sensitivity was 100% for all smear-positive, M. tuberculosis culture-positive specimens for both the HBTC and the ATC. Of smear-negative, M. tuberculosis culture-positive specimens, sensitivity was 42.9% with the HBTC and 22.0% with the ATC. Specificity was 100% for both assay systems. Total assay time was 6.5 and 4 h and the reagent cost was 84 and 32 cents for the HBTC and ATC, respectively. The ATC offered an excellent alternative to the traditional HBTC for diagnosis of M. tuberculosis in smear-positive specimens by PCR.

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Year:  1997        PMID： 9230404      PMCID： PMC229925          DOI： 10.1128/jcm.35.8.2157-2159.1997

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  16 in total

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Authors:  S Kozaki; J Onimaru; Y Kamata; G Sakaguchi
Journal:  Infect Immun       Date:  1991-01       Impact factor: 3.441

2.  Characterization of a Mycobacterium tuberculosis insertion sequence, IS6110, and its application in diagnosis.

Authors:  D Thierry; A Brisson-Noël; V Vincent-Lévy-Frébault; S Nguyen; J L Guesdon; B Gicquel
Journal:  J Clin Microbiol       Date:  1990-12       Impact factor: 5.948

3.  Sequences of the botulinal neurotoxin E derived from Clostridium botulinum type E (strain Beluga) and Clostridium butyricum (strains ATCC 43181 and ATCC 43755).

Authors:  S Poulet; D Hauser; M Quanz; H Niemann; M R Popoff
Journal:  Biochem Biophys Res Commun       Date:  1992-02-28       Impact factor: 3.575

4.  Prevalence of Clostridium botulinum type E and coexistence of C. botulinum nonproteolytic type B in the river soil of Japan.

Authors:  K Yamakawa; S Nakamura
Journal:  Microbiol Immunol       Date:  1992       Impact factor: 1.955

5.  Detection of Mycobacterium tuberculosis in sputum samples using a polymerase chain reaction.

Authors:  K D Eisenach; M D Sifford; M D Cave; J H Bates; J T Crawford
Journal:  Am Rev Respir Dis       Date:  1991-11

6.  Characterization of an organism that produces type E botulinal toxin but which resembles Clostridium butyricum from the feces of an infant with type E botulism.

Authors:  L M McCroskey; C L Hatheway; L Fenicia; B Pasolini; P Aureli
Journal:  J Clin Microbiol       Date:  1986-01       Impact factor: 5.948

7.  Detection of Mycobacterium tuberculosis in clinical samples by two-step polymerase chain reaction and nonisotopic hybridization methods.

Authors:  R M Shawar; F A el-Zaatari; A Nataraj; J E Clarridge
Journal:  J Clin Microbiol       Date:  1993-01       Impact factor: 5.948

8.  Isolation of an organism resembling Clostridium barati which produces type F botulinal toxin from an infant with botulism.

Authors:  J D Hall; L M McCroskey; B J Pincomb; C L Hatheway
Journal:  J Clin Microbiol       Date:  1985-04       Impact factor: 5.948

9.  Two cases of type E infant botulism caused by neurotoxigenic Clostridium butyricum in Italy.

Authors:  P Aureli; L Fenicia; B Pasolini; M Gianfranceschi; L M McCroskey; C L Hatheway
Journal:  J Infect Dis       Date:  1986-08       Impact factor: 5.226

10.  Comparison of toxins of Clostridium butyricum and Clostridium botulinum type E.

Authors:  J A Giménez; H Sugiyama
Journal:  Infect Immun       Date:  1988-04       Impact factor: 3.441
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  5 in total

1.  A rapid polymerase chain reaction technique for detecting M tuberculosis in a variety of clinical specimens.

Authors:  A M Kearns; R Freeman; M Steward; J G Magee
Journal:  J Clin Pathol       Date:  1998-12       Impact factor: 3.411

2.  Evaluation of a rapid air thermal cycler for detection of Mycobacterium tuberculosis.

Authors:  A M Kearns; R Freeman; M Steward
Journal:  J Clin Microbiol       Date:  1998-02       Impact factor: 5.948

3.  Detection of Mycobacterium bovis in bovine clinical specimens using real-time fluorescence and fluorescence resonance energy transfer probe rapid-cycle PCR.

Authors:  M J Taylor; M S Hughes; R A Skuce; S D Neill
Journal:  J Clin Microbiol       Date:  2001-04       Impact factor: 5.948

4.  Rapid-cycle PCR and fluorimetry for detection of mycobacteria.

Authors:  Jacqueline Lachnik; Birgit Ackermann; Antje Bohrssen; Silvia Maass; Catharina Diephaus; Axel Puncken; Marion Stermann; Franz-Christoph Bange
Journal:  J Clin Microbiol       Date:  2002-09       Impact factor: 5.948

5.  Rapid DNA amplification using a battery-powered thin-film resistive thermocycler.

Authors:  Keith E Herold; Nikolay Sergeev; Andriy Matviyenko; Avraham Rasooly
Journal:  Methods Mol Biol       Date:  2009
  5 in total

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