Literature DB >> 10070334

A rapid polymerase chain reaction technique for detecting M tuberculosis in a variety of clinical specimens.

A M Kearns1, R Freeman, M Steward, J G Magee.   

Abstract

A rapid in-house polymerase chain reaction (PCR) assay is described for the direct detection of Mycobacterium tuberculosis complex in clinical material. Its performance is compared with two kit based systems. The results of the in-house assay were comparable with the commercial assays, detecting M tuberculosis in 100% of smear positive, culture positive samples. The in-house assay proved to be rapid, easy, and inexpensive to perform, and the inclusion of an internal inhibitor control permitted validation of the PCR results.

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Year:  1998        PMID： 10070334      PMCID： PMC501028          DOI： 10.1136/jcp.51.12.922

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN： 0021-9746            Impact factor:   3.411


  10 in total

1.  Evaluation of a rapid air thermal cycler for detection of Mycobacterium tuberculosis.

Authors:  A M Kearns; R Freeman; M Steward
Journal:  J Clin Microbiol       Date:  1998-02       Impact factor: 5.948

Review 2.  Acyclic nucleoside phosphonates: a new dimension to the chemotherapy of DNA virus and retrovirus infections.

Authors:  E De Clercq
Journal:  J Med Microbiol       Date:  1998-01       Impact factor: 2.472

3.  Evaluation of a novel PCR-based diagnostic assay for detection of Mycobacterium tuberculosis in sputum samples.

Authors:  M Maher; M Glennon; G Martinazzo; E Turchetti; S Marcolini; T Smith; M T Dawson
Journal:  J Clin Microbiol       Date:  1996-09       Impact factor: 5.948

4.  Comparison of the amplified Mycobacterium tuberculosis (MTB) direct test, Amplicor MTB PCR, and IS6110-PCR for detection of MTB in respiratory specimens.

Authors:  J R Dalovisio; S Montenegro-James; S A Kemmerly; C F Genre; R Chambers; D Greer; G A Pankey; D M Failla; K G Haydel; L Hutchinson; M F Lindley; B M Nunez; A Praba; K D Eisenach; E S Cooper
Journal:  Clin Infect Dis       Date:  1996-11       Impact factor: 9.079

5.  Evaluation of a rapid air thermal cycler for detection of Mycobacterium tuberculosis.

Authors:  K Chapin; T L Lauderdale
Journal:  J Clin Microbiol       Date:  1997-08       Impact factor: 5.948

6.  Comparison of two automated DNA amplification systems with a manual one-tube nested PCR assay for diagnosis of pulmonary tuberculosis.

Authors:  K Y Yuen; W C Yam; L P Wong; W H Seto
Journal:  J Clin Microbiol       Date:  1997-06       Impact factor: 5.948

7.  Comparative usefulness of PCR in the detection of Mycobacterium tuberculosis in different clinical specimens.

Authors:  M F Tan; W C Ng; S H Chan; W C Tan
Journal:  J Med Microbiol       Date:  1997-02       Impact factor: 2.472

8.  Substances interfering with direct detection of Mycobacterium tuberculosis in clinical specimens by PCR: effects of bovine serum albumin.

Authors:  B A Forbes; K E Hicks
Journal:  J Clin Microbiol       Date:  1996-09       Impact factor: 5.948

9.  Diagnosis of tuberculosis by DNA amplification in clinical practice evaluation.

Authors:  A Brisson-Noel; C Aznar; C Chureau; S Nguyen; C Pierre; M Bartoli; R Bonete; G Pialoux; B Gicquel; G Garrigue
Journal:  Lancet       Date:  1991-08-10       Impact factor: 79.321

10.  Evaluation of Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test and PCR for direct detection of Mycobacterium tuberculosis in clinical specimens.

Authors:  N Miller; S G Hernandez; T J Cleary
Journal:  J Clin Microbiol       Date:  1994-02       Impact factor: 5.948
  10 in total
  12 in total

1.  PCR to detect M tuberculosis.

Authors:  O M Jolobe
Journal:  J Clin Pathol       Date:  1999-05       Impact factor: 3.411

2.  Identifying sputum specimens of high priority for examination by enhanced mycobacterial detection, identification, and susceptibility systems (EMDISS) to promote the rapid diagnosis of infectious pulmonary tuberculosis.

Authors:  R Freeman; J Magee; A Barrett
Journal:  J Clin Pathol       Date:  2001-08       Impact factor: 3.411

3.  Clinical evaluation of the polymerase chain reaction for the rapid diagnosis of tuberculosis.

Authors:  V C C Cheng; W C Yam; I F N Hung; P C Y Woo; S K P Lau; B S F Tang; K Y Yuen
Journal:  J Clin Pathol       Date:  2004-03       Impact factor: 3.411

4.  Novel multipurpose methodology for detection of mycobacteria in pulmonary and extrapulmonary specimens by smear microscopy, culture, and PCR.

Authors:  Soumitesh Chakravorty; Jaya Sivaswami Tyagi
Journal:  J Clin Microbiol       Date:  2005-06       Impact factor: 5.948

Review 5.  Current evidence on diagnostic accuracy of commercially based nucleic acid amplification tests for the diagnosis of pulmonary tuberculosis.

Authors:  S Greco; E Girardi; A Navarra; C Saltini
Journal:  Thorax       Date:  2006-05-31       Impact factor: 9.139

6.  Diagnostic accuracy of in-house PCR for pulmonary tuberculosis in smear-positive patients: meta-analysis and metaregression.

Authors:  S Greco; M Rulli; E Girardi; C Piersimoni; C Saltini
Journal:  J Clin Microbiol       Date:  2009-01-14       Impact factor: 5.948

Review 7.  Establishing the diagnosis of tuberculous vertebral osteomyelitis.

Authors:  Juan D Colmenero; Juan D Ruiz-Mesa; Rocío Sanjuan-Jimenez; Beatriz Sobrino; Pilar Morata
Journal:  Eur Spine J       Date:  2012-05-11       Impact factor: 3.134

8.  Novel real-time simultaneous amplification and testing method to accurately and rapidly detect Mycobacterium tuberculosis complex.

Authors:  Zhenling Cui; Yongzhong Wang; Liang Fang; Ruijuan Zheng; Xiaochen Huang; Xiaoqin Liu; Gang Zhang; Dongmei Rui; Jinliang Ju; Zhongyi Hu
Journal:  J Clin Microbiol       Date:  2012-01-11       Impact factor: 5.948

9.  Detection of Mycobacterium bovis in bovine clinical specimens using real-time fluorescence and fluorescence resonance energy transfer probe rapid-cycle PCR.

Authors:  M J Taylor; M S Hughes; R A Skuce; S D Neill
Journal:  J Clin Microbiol       Date:  2001-04       Impact factor: 5.948

10.  Assessment by meta-analysis of PCR for diagnosis of smear-negative pulmonary tuberculosis.

Authors:  Olga L Sarmiento; Kristen A Weigle; Janet Alexander; David J Weber; William C Miller
Journal:  J Clin Microbiol       Date:  2003-07       Impact factor: 5.948
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