Literature DB >> 9199939

Centromeric and noncentromeric ADE2-selectable fragmentation vectors for yeast artificial chromosomes in AB1380.

J J Heus1, M P de Winther, E van de Vosse, G J van Ommen, J T den Dunnen.   

Abstract

We have constructed a set of fragmentation vectors for the truncation of either the centromeric or the noncentromeric end of YACs containing a human DNA insert. These vectors carry ADE2 or HIS5 as the selectable marker, enabling direct use in AB1380, the host strain of most publicly available YAC libraries. Centromeric fragmentation vectors for AB1380 have not been reported previously; the noncentromeric vectors show high frequencies of fragmentation.

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Year:  1997        PMID： 9199939      PMCID： PMC310663          DOI： 10.1101/gr.7.6.657

Source DB:  PubMed          Journal:  Genome Res        ISSN： 1088-9051            Impact factor:   9.043


  13 in total

1.  Yeast artificial chromosome libraries containing large inserts from mouse and human DNA.

Authors:  Z Larin; A P Monaco; H Lehrach
Journal:  Proc Natl Acad Sci U S A       Date:  1991-05-15       Impact factor: 11.205

2.  Construction and characterization of a yeast artificial chromosome library containing seven haploid human genome equivalents.

Authors:  H M Albertsen; H Abderrahim; H M Cann; J Dausset; D Le Paslier; D Cohen
Journal:  Proc Natl Acad Sci U S A       Date:  1990-06       Impact factor: 11.205

3.  Construction of yeast artificial chromosome libraries with large inserts using fractionation by pulsed-field gel electrophoresis.

Authors:  R Anand; A Villasante; C Tyler-Smith
Journal:  Nucleic Acids Res       Date:  1989-05-11       Impact factor: 16.971

4.  High-resolution mapping by YAC fragmentation of a 2.5-Mb Xp22 region containing the human RS, KFSD and CLS disease genes.

Authors:  E Van de Vosse; P Van der Bent; J J Heus; G J Van Ommen; J T Den Dunnen
Journal:  Mamm Genome       Date:  1997-07       Impact factor: 2.957

5.  Generation of deletion derivatives by targeted transformation of human-derived yeast artificial chromosomes.

Authors:  W J Pavan; P Hieter; R H Reeves
Journal:  Proc Natl Acad Sci U S A       Date:  1990-02       Impact factor: 11.205

6.  The ADE2 gene from Saccharomyces cerevisiae: sequence and new vectors.

Authors:  A Stotz; P Linder
Journal:  Gene       Date:  1990-10-30       Impact factor: 3.688

7.  Creation of a yeast artificial chromosome fragmentation vector based on lysine-2.

Authors:  B C Lewis; N P Shah; B S Braun; C T Denny
Journal:  Genet Anal Tech Appl       Date:  1992-06

8.  New vectors for manipulation and selection of functional yeast artificial chromosomes (YACs) containing human DNA inserts.

Authors:  S L Emanuel; J R Cook; J O'Rear; R Rothstein; S Pestka
Journal:  Gene       Date:  1995-04-03       Impact factor: 3.688

9.  Yeast kar1 mutants provide an effective method for YAC transfer to new hosts.

Authors:  F Spencer; Y Hugerat; G Simchen; O Hurko; C Connelly; P Hieter
Journal:  Genomics       Date:  1994-07-01       Impact factor: 5.736

10.  Centromeric DNA from chromosome VI in Saccharomyces cerevisiae strains.

Authors:  L Panzeri; P Philippsen
Journal:  EMBO J       Date:  1982       Impact factor: 11.598
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  1 in total

1.  High-resolution mapping by YAC fragmentation of a 2.5-Mb Xp22 region containing the human RS, KFSD and CLS disease genes.

Authors:  E Van de Vosse; P Van der Bent; J J Heus; G J Van Ommen; J T Den Dunnen
Journal:  Mamm Genome       Date:  1997-07       Impact factor: 2.957
  1 in total

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