Literature DB >> 9185581

Recovery of YAC-end sequences through complementation of an Escherichia coli pyrF mutation.

D A Wright1, S K Park, D Wu, G J Phillips, S R Rodermel, D F Voytas.   

Abstract

We have developed a genetic means to recover sequences from YAC-ends near the yeast selectable marker URA3. This strategy is based on the ability of URA3 to complement mutations in pyrF, an Escherichia coli gene required for pyrimidine biosynthesis. We have developed an E.coli strain with a non-reverting allele of pyrF that is also suitable for cloning (recA-, hsdR-). We demonstrate the utility of this complementation strategy to obtain right-end clones from three YACs containing Arabidopsis thaliana DNA.

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Year:  1997        PMID： 9185581      PMCID： PMC146780          DOI： 10.1093/nar/25.13.2679

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  8 in total

1.  A novel, rapid method for the isolation of terminal sequences from yeast artificial chromosome (YAC) clones.

Authors:  J Riley; R Butler; D Ogilvie; R Finniear; D Jenner; S Powell; R Anand; J C Smith; A F Markham
Journal:  Nucleic Acids Res       Date:  1990-05-25       Impact factor: 16.971

2.  Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors.

Authors:  D T Burke; G F Carle; M V Olson
Journal:  Science       Date:  1987-05-15       Impact factor: 47.728

3.  Development of large DNA methods for plants: molecular cloning of large segments of Arabidopsis and carrot DNA into yeast.

Authors:  P Guzmán; J R Ecker
Journal:  Nucleic Acids Res       Date:  1988-12-09       Impact factor: 16.971

4.  Genetic applications of an inverse polymerase chain reaction.

Authors:  H Ochman; A S Gerber; D L Hartl
Journal:  Genetics       Date:  1988-11       Impact factor: 4.562

5.  Evidence for transcriptional regulation of orotidine-5'-phosphate decarboxylase in yeast by hybridization of mRNA to the yeast structural gene cloned in Escherichia coli.

Authors:  M L Bach; F Lacroute; D Botstein
Journal:  Proc Natl Acad Sci U S A       Date:  1979-01       Impact factor: 11.205

6.  Rescue of end fragments of yeast artificial chromosomes by homologous recombination in yeast.

Authors:  G G Hermanson; M F Hoekstra; D L McElligott; G A Evans
Journal:  Nucleic Acids Res       Date:  1991-09-25       Impact factor: 16.971

7.  The CIC library: a large insert YAC library for genome mapping in Arabidopsis thaliana.

Authors:  F Creusot; E Fouilloux; M Dron; J Lafleuriel; G Picard; A Billault; D Le Paslier; D Cohen; M E Chabouté; A Durr
Journal:  Plant J       Date:  1995-11       Impact factor: 6.417

8.  A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

Authors:  R S Sikorski; P Hieter
Journal:  Genetics       Date:  1989-05       Impact factor: 4.562
  8 in total
  2 in total

1.  The IMMUTANS variegation locus of Arabidopsis defines a mitochondrial alternative oxidase homolog that functions during early chloroplast biogenesis.

Authors:  D Wu; D A Wright; C Wetzel; D F Voytas; S Rodermel
Journal:  Plant Cell       Date:  1999-01       Impact factor: 11.277

2.  Francisella tularensis DeltapyrF mutants show that replication in nonmacrophages is sufficient for pathogenesis in vivo.

Authors:  Joseph Horzempa; Dawn M O'Dee; Robert M Q Shanks; Gerard J Nau
Journal:  Infect Immun       Date:  2010-04-12       Impact factor: 3.441
  2 in total

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