Literature DB >> 3060856

Development of large DNA methods for plants: molecular cloning of large segments of Arabidopsis and carrot DNA into yeast.

P Guzmán1, J R Ecker.   

Abstract

Procedures for the preparation, analysis and cloning of large DNA molecules from two different plant species are described. Arabidopsis and carrot protoplasts were used for the preparation of large DNA molecules in agarose "plugs" or in solution. Pulsed-field gel electrophoresis (PFGE) analysis of large plant DNA preparations using a contour-clamped homogeneous field (CHEF) apparatus indicated that the size of the DNA was at least 12 Mb. Large DNA preparations were shown to be useful for restriction enzyme analysis of the Arabidopsis genome using both frequent and infrequent cutting enzymes and for the molecular cloning of large segments of DNA into yeast using artificial chromosome (YAC) vectors. PFGE and blot hybridization analysis of Arabidopsis and carrot DNA-containing YACs indicated that both unique and highly repeated DNA sequences were represented in these libraries.

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Year:  1988        PMID： 3060856      PMCID： PMC338998          DOI： 10.1093/nar/16.23.11091

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  29 in total

1.  A Genetic Map of Lettuce (Lactuca sativa L.) with Restriction Fragment Length Polymorphism, Isozyme, Disease Resistance and Morphological Markers.

Authors:  B S Landry; R V Kesseli; B Farrara; R W Michelmore
Journal:  Genetics       Date:  1987-06       Impact factor: 4.562

2.  Strategies for mapping and cloning macroregions of mammalian genomes.

Authors:  C L Smith; S K Lawrance; G A Gillespie; C R Cantor; S M Weissman; F S Collins
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

Review 3.  Arabidopsis thaliana.

Authors:  E M Meyerowitz
Journal:  Annu Rev Genet       Date:  1987       Impact factor: 16.830

4.  Directional cloning of DNA fragments at a large distance from an initial probe: a circularization method.

Authors:  F S Collins; S M Weissman
Journal:  Proc Natl Acad Sci U S A       Date:  1984-11       Impact factor: 11.205

5.  Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis.

Authors:  G F Carle; M V Olson
Journal:  Nucleic Acids Res       Date:  1984-07-25       Impact factor: 16.971

6.  Physical mapping of large DNA by chromosome fragmentation.

Authors:  D Vollrath; R W Davis; C Connelly; P Hieter
Journal:  Proc Natl Acad Sci U S A       Date:  1988-08       Impact factor: 11.205

7.  Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.

Authors:  D C Schwartz; C R Cantor
Journal:  Cell       Date:  1984-05       Impact factor: 41.582

8.  A fission yeast chromosome can replicate autonomously in mouse cells.

Authors:  R C Allshire; G Cranston; J R Gosden; J C Maule; N D Hastie; P A Fantes
Journal:  Cell       Date:  1987-07-31       Impact factor: 41.582

Review 9.  Construction of a genetic linkage map in man using restriction fragment length polymorphisms.

Authors:  D Botstein; R L White; M Skolnick; R W Davis
Journal:  Am J Hum Genet       Date:  1980-05       Impact factor: 11.025

10.  Isolation of candidate cDNAs for portions of the Duchenne muscular dystrophy gene.

Authors:  A P Monaco; R L Neve; C Colletti-Feener; C J Bertelson; D M Kurnit; L M Kunkel
Journal:  Nature       Date:  1986 Oct 16-22       Impact factor: 49.962
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  34 in total

1.  Construction of a yeast artificial chromosome library of tomato and identification of cloned segments linked to two disease resistance loci.

Authors:  G B Martin; M W Ganal; S D Tanksley
Journal:  Mol Gen Genet       Date:  1992-05

2.  Amplification of large artificial chromosomes.

Authors:  D R Smith; A P Smyth; D T Moir
Journal:  Proc Natl Acad Sci U S A       Date:  1990-11       Impact factor: 11.205

3.  Entire maize chloroplast genome is stably maintained in a yeast artificial chromosome.

Authors:  M Gupta; B Hoo
Journal:  Plant Mol Biol       Date:  1991-09       Impact factor: 4.076

4.  Preparation of high molecular weight plant DNA and its use for artificial chromosome construction.

Authors:  A Balestrazzi; G Bernacchia; R Cella; L Ferretti; S Sora
Journal:  Plant Cell Rep       Date:  1991-09       Impact factor: 4.570

5.  Introduction of large linear minichromosomes into Schizosaccharomyces pombe by an improved transformation procedure.

Authors:  R C Allshire
Journal:  Proc Natl Acad Sci U S A       Date:  1990-06       Impact factor: 11.205

6.  The Arabidopsis HUELLENLOS gene, which is essential for normal ovule development, encodes a mitochondrial ribosomal protein.

Authors:  D J Skinner; S C Baker; R J Meister; J Broadhvest; K Schneitz; C S Gasser
Journal:  Plant Cell       Date:  2001-12       Impact factor: 11.277

7.  Isolation of single-copy-sequence clones from a yeast artificial chromosome library of randomly-sheared Arabidopsis thaliana DNA.

Authors:  E R Ward; G C Jen
Journal:  Plant Mol Biol       Date:  1990-04       Impact factor: 4.076

8.  Construction of a YAC library from a Beta vulgaris fragment addition and isolation of a major satellite DNA cluster linked to the beet cyst nematode resistance locus Hs1 (pat-1.).

Authors:  R M Klein-Lankhorst; E M Salentijn; W G Dirkse; M Arens-de Reuver; W J Stiekema
Journal:  Theor Appl Genet       Date:  1994-10       Impact factor: 5.699

9.  Physical mapping and cloning of a translocation in sugar beet (Beta vulgaris L) carrying a gene for nematode (Heterodera schachtii) resistance from B. procumbens.

Authors:  M Kleine; D Cai; C Elbl; R G Herrmann; C Jung
Journal:  Theor Appl Genet       Date:  1995-03       Impact factor: 5.699

10.  A 3.5 genome equivalent multi access YAC library: construction, characterisation, screening and storage.

Authors:  R Anand; J H Riley; R Butler; J C Smith; A F Markham
Journal:  Nucleic Acids Res       Date:  1990-04-25       Impact factor: 16.971
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