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Literature DB >> 9157172

Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model.

L Monteiro¹, D Bonnemaison, A Vekris, K G Petry, J Bonnet, R Vidal, J Cabrita, F Mégraud.

Abstract

A model was developed to study inhibitors present in feces which prevent the use of PCR for the detection of Helicobacter pylori. A DNA fragment amplified with the same primers as H. pylori was used to spike samples before extraction by a modified QIAamp tissue method. Inhibitors, separated on an Ultrogel AcA44 column, were characterized. Inhibitors in feces are complex polysaccharides possibly originating from vegetable material in the diet.

Entities: Chemical Species

Mesh：

Substances：
Polysaccharides

Year: 1997 PMID： 9157172 PMCID： PMC229720 DOI： 10.1128/jcm.35.4.995-998.1997

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

19 in total

1. The effects of plant polysaccharides and buffer additives on PCR.

Authors: T Demeke; R P Adams
Journal: Biotechniques Date: 1992-03 Impact factor: 1.993

2. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material.

Authors: P S Walsh; D A Metzger; R Higuchi
Journal: Biotechniques Date: 1991-04 Impact factor: 1.993

3. Direct polymerase chain reaction test for detection of Helicobacter pylori in humans and animals.

Authors: S A Ho; J A Hoyle; F A Lewis; A D Secker; D Cross; N P Mapstone; M F Dixon; J I Wyatt; D S Tompkins; G R Taylor
Journal: J Clin Microbiol Date: 1991-11 Impact factor: 5.948

4. A simple technique for removing plant polysaccharide contaminants from DNA.

Authors: N Do; R P Adams
Journal: Biotechniques Date: 1991-02 Impact factor: 1.993

5. Removal of inhibitory substances from human fecal specimens for detection of group A rotaviruses by reverse transcriptase and polymerase chain reactions.

Authors: J Wilde; J Eiden; R Yolken
Journal: J Clin Microbiol Date: 1990-06 Impact factor: 5.948

6. Immunomagnetic separation and DNA hybridization for detection of enterotoxigenic Escherichia coli in a piglet model.

Authors: A Lund; Y Wasteson; O Olsvik
Journal: J Clin Microbiol Date: 1991-10 Impact factor: 5.948

7. Influences of anionic polysaccharides on DNA synthesis in isolated nuclei and by DNA polymerase alpha: correlation of observed effects with properties of the polysaccharides.

Authors: K Furukawa; V P Bhavanandan
Journal: Biochim Biophys Acta Date: 1983-09-09

8. Rapid detection of Helicobacter pylori in gastric biopsy material by polymerase chain reaction.

Authors: M Hammar; T Tyszkiewicz; T Wadström; P W O'Toole
Journal: J Clin Microbiol Date: 1992-01 Impact factor: 5.948

9. Polymerase chain reaction for detection of the cholera enterotoxin operon of Vibrio cholerae.

Authors: H Shirai; M Nishibuchi; T Ramamurthy; S K Bhattacharya; S C Pal; Y Takeda
Journal: J Clin Microbiol Date: 1991-11 Impact factor: 5.948

10. Sequence-selective carbohydrate-DNA interaction: dimeric and monomeric forms of the calicheamicin oligosaccharide interfere with transcription factor function.

Authors: C Liu; B M Smith; K Ajito; H Komatsu; L Gomez-Paloma; T Li; E A Theodorakis; K C Nicolaou; P K Vogt
Journal: Proc Natl Acad Sci U S A Date: 1996-01-23 Impact factor: 11.205

128 in total

1. Fluorescence in situ hybridization method using a peptide nucleic acid probe for identification of Salmonella spp. in a broad spectrum of samples.

Authors: C Almeida; N F Azevedo; R M Fernandes; C W Keevil; M J Vieira
Journal: Appl Environ Microbiol Date: 2010-05-07 Impact factor: 4.792

2. Early detection and estimation of infection burden by real-time PCR in rats experimentally infected with Clonorchis sinensis.

Authors: S M Mazidur Rahman; Young Mee Bae; Sung-Tae Hong; Min-Ho Choi
Journal: Parasitol Res Date: 2011-01-29 Impact factor: 2.289

Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model.

1. The effects of plant polysaccharides and buffer additives on PCR.

2. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material.

3. Direct polymerase chain reaction test for detection of Helicobacter pylori in humans and animals.

4. A simple technique for removing plant polysaccharide contaminants from DNA.

5. Removal of inhibitory substances from human fecal specimens for detection of group A rotaviruses by reverse transcriptase and polymerase chain reactions.

6. Immunomagnetic separation and DNA hybridization for detection of enterotoxigenic Escherichia coli in a piglet model.

7. Influences of anionic polysaccharides on DNA synthesis in isolated nuclei and by DNA polymerase alpha: correlation of observed effects with properties of the polysaccharides.

8. Rapid detection of Helicobacter pylori in gastric biopsy material by polymerase chain reaction.

9. Polymerase chain reaction for detection of the cholera enterotoxin operon of Vibrio cholerae.

10. Sequence-selective carbohydrate-DNA interaction: dimeric and monomeric forms of the calicheamicin oligosaccharide interfere with transcription factor function.

1. Fluorescence in situ hybridization method using a peptide nucleic acid probe for identification of Salmonella spp. in a broad spectrum of samples.

2. Early detection and estimation of infection burden by real-time PCR in rats experimentally infected with Clonorchis sinensis.

3. Applications of competitor RNA in diagnostic reverse transcription-PCR.

Review 4. Pre-PCR processing: strategies to generate PCR-compatible samples.

5. Rapid detection of methicillin-resistant staphylococci from blood culture bottles by using a multiplex PCR assay.

6. Utilization of Small RNA Genes to Distinguish Vibrio cholerae Biotypes via Multiplex Polymerase Chain Reaction.

7. Colonization of cattle intestines by Campylobacter jejuni and Campylobacter lanienae.

8. Real-time PCR for quantifying Haemonchus contortus eggs and potential limiting factors.

9. A Novel Real-Time PCR-Based Screening Test with Pooled Fecal Samples for Bovine Johne's Disease.

10. Development of a noninvasive method for detecting and monitoring the time course of Helicobacter pylori infection.