Literature DB >> 9153331

Efficient combination of large DNA in vitro: in gel site specific recombination (IGSSR) of PAC fragments containing alpha satellite DNA and the human HPRT gene locus.

D Schindelhauer1, H J Cooke.   

Abstract

In an attempt to combine a cloned genomic copy of a selectable gene with different cloned centromeric sequences to develop mammalian artificial chromosomes (MAC) we used site specific recombination mediated by purified Cre recombinase acting on the loxP sequence in PAC vector DNA. A new method was required to purify highly concentrated, virtually 100% intact PAC DNA which could be stored for a long period. Here we show the efficient linking of linearized PACs containing alpha satellite DNA from chromosomes X and 17 with sizes of 125 and 140 kb, respectively, to a 95 kb restriction fragment derived from a 175 kb PAC containing the intact human HPRT gene locus.

Entities:  

Mesh：

Substances：

Year:  1997        PMID： 9153331      PMCID： PMC146721          DOI： 10.1093/nar/25.11.2241

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  12 in total

1.  Cre-lox recombination in Escherichia coli cells. Mechanistic differences from the in vitro reaction.

Authors:  D E Adams; J B Bliska; N R Cozzarelli
Journal:  J Mol Biol       Date:  1992-08-05       Impact factor: 5.469

2.  Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector.

Authors:  H Shizuya; B Birren; U J Kim; V Mancino; T Slepak; Y Tachiiri; M Simon
Journal:  Proc Natl Acad Sci U S A       Date:  1992-09-15       Impact factor: 11.205

3.  Retrofitting vectors for Escherichia coli-based artificial chromosomes (PACs and BACs) with markers for transfection studies.

Authors:  J E Mejía; A P Monaco
Journal:  Genome Res       Date:  1997-02       Impact factor: 9.043

4.  Different thermostabilities of FLP and Cre recombinases: implications for applied site-specific recombination.

Authors:  F Buchholz; L Ringrose; P O Angrand; F Rossi; A F Stewart
Journal:  Nucleic Acids Res       Date:  1996-11-01       Impact factor: 16.971

5.  The role of the loxP spacer region in P1 site-specific recombination.

Authors:  R H Hoess; A Wierzbicki; K Abremski
Journal:  Nucleic Acids Res       Date:  1986-03-11       Impact factor: 16.971

Review 6.  YACs, BACs, PACs and MACs: artificial chromosomes as research tools.

Authors:  A P Monaco; Z Larin
Journal:  Trends Biotechnol       Date:  1994-07       Impact factor: 19.536

7.  Generation of large insert YAC libraries.

Authors:  Z Larin; A P Monaco; H Lehrach
Journal:  Methods Mol Biol       Date:  1996

8.  A new bacteriophage P1-derived vector for the propagation of large human DNA fragments.

Authors:  P A Ioannou; C T Amemiya; J Garnes; P M Kroisel; H Shizuya; C Chen; M A Batzer; P J de Jong
Journal:  Nat Genet       Date:  1994-01       Impact factor: 38.330

9.  Studies on the properties of P1 site-specific recombination: evidence for topologically unlinked products following recombination.

Authors:  K Abremski; R Hoess; N Sternberg
Journal:  Cell       Date:  1983-04       Impact factor: 41.582

10.  Site-specific recombination by the bacteriophage P1 lox-Cre system. Cre-mediated synapsis of two lox sites.

Authors:  D L Hamilton; K Abremski
Journal:  J Mol Biol       Date:  1984-09-15       Impact factor: 5.469
View more
  2 in total

1.  Evidence for a fast, intrachromosomal conversion mechanism from mapping of nucleotide variants within a homogeneous alpha-satellite DNA array.

Authors:  Dirk Schindelhauer; Tobias Schwarz
Journal:  Genome Res       Date:  2002-12       Impact factor: 9.043

Review 2.  Artificial and engineered chromosomes: developments and prospects for gene therapy.

Authors:  Brenda R Grimes; Zoia Larin Monaco
Journal:  Chromosoma       Date:  2005-10-15       Impact factor: 4.316
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.