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Literature DB >> 9115372

Angle and locus of the bend induced by the msp I DNA methyltransferase in a sequence-specific complex with DNA.

Abstract

Bending of DNA induced by M.Msp I, one of the m5C-DNA methyltransferases, has been investigated using circular permutation analysis. The M.Msp I MTase induced sharp bends in DNA containing its recognition sequence 5'-CCGG-3'which was estimated to be 142+/-4 degrees and 132+/-4 degrees for circularly permuted DNA fragments of 127 and 1459 bp respectively. The bend centre was found to be asymmetric with respect to the CCGG sequence and appeared to exclude the 'target cytosine'. An estimate of approximately 15 kcal/mol was obtained for the free energy associated with M.Msp I-induced DNA bending.

Entities: Chemical

Mesh：

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Year: 1997 PMID： 9115372 PMCID： PMC146686 DOI： 10.1093/nar/25.10.2025

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

28 in total

1. Conformational fluctuations of DNA helix.

Authors: D E Depew; J C Wang
Journal: Proc Natl Acad Sci U S A Date: 1975-11 Impact factor: 11.205

2. Sequence-specific recognition of cytosine C5 and adenine N6 DNA methyltransferases requires different deformations of DNA.

Authors: R A Garcia; C J Bustamante; N O Reich
Journal: Proc Natl Acad Sci U S A Date: 1996-07-23 Impact factor: 11.205

3. On the sequence determinants and flexibility of the kinetoplast DNA fragment with abnormal gel electrophoretic mobilities.

Authors: S Diekmann; J C Wang
Journal: J Mol Biol Date: 1985-11-05 Impact factor: 5.469

Angle and locus of the bend induced by the msp I DNA methyltransferase in a sequence-specific complex with DNA.

1. Conformational fluctuations of DNA helix.

2. Sequence-specific recognition of cytosine C5 and adenine N6 DNA methyltransferases requires different deformations of DNA.

3. On the sequence determinants and flexibility of the kinetoplast DNA fragment with abnormal gel electrophoretic mobilities.

4. Multiple DNA-protein interactions governing high-precision DNA transactions.

5. DNA bending at adenine . thymine tracts.

6. 'Interactive' recognition in EcoRI restriction enzyme-DNA complex.

7. The organization and complete nucleotide sequence of the PstI restriction-modification system.

8. Theory of gel electrophoresis of DNA.

9. Temperature and salt dependence of the gel migration anomaly of curved DNA fragments.

10. The DNA binding domain and bending angle of E. coli CAP protein.

1. DNA bending induced by DNA (cytosine-5) methyltransferases.

2. Kinetic Basis of the Bifunctionality of SsoII DNA Methyltransferase.