Literature DB >> 9114417

Development of a reverse transcriptase PCR-enzyme-linked immunosorbent assay for quantification of human immunodeficiency virus type 1 RNA in plasma: comparison with commercial quantitative assays.

M A Trabaud1, G Audoly, K Leriche, L Cotte, J Ritter, M Sepetjan, C Trepo.   

Abstract

A quantitative reverse transcriptase PCR assay with automated detection by nonradioactive hybridization was developed for the determination of human immunodeficiency virus (HIV) type 1 RNA levels. This assay is based on the use of an external standard curve with an internal standard. The accuracy of quantification was verified by comparison with reference commercial tests, the Chiron branched-DNA and Roche AMPLICOR HIV MONITOR assays. This assay was able to quantify viremia in patients with CD4 cell numbers below and above 500/mm3 and to quantify some HIV strains which could not be titrated by the MONITOR assay.

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Year:  1997        PMID： 9114417      PMCID： PMC232739          DOI： 10.1128/jcm.35.5.1251-1254.1997

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  13 in total

1.  A novel procedure for quantitative polymerase chain reaction by coamplification of competitive templates.

Authors:  S Diviacco; P Norio; L Zentilin; S Menzo; M Clementi; G Biamonti; S Riva; A Falaschi; M Giacca
Journal:  Gene       Date:  1992-12-15       Impact factor: 3.688

2.  Detection of HIV-1 DNA by PCR: evaluation of primer pair concordance and sensitivity of a single primer pair.

Authors:  C E Lynch; R Madej; P H Louie; G Rodgers
Journal:  J Acquir Immune Defic Syndr (1988)       Date:  1992

3.  Absolute quantitation of viremia in human immunodeficiency virus infection by competitive reverse transcription and polymerase chain reaction.

Authors:  S Menzo; P Bagnarelli; M Giacca; A Manzin; P E Varaldo; M Clementi
Journal:  J Clin Microbiol       Date:  1992-07       Impact factor: 5.948

4.  Plasma viraemia as a marker of viral replication in HIV-infected individuals.

Authors:  S Escaich; J Ritter; P Rougier; D Lepot; J P Lamelin; M Sepetjan; C Trepo
Journal:  AIDS       Date:  1991-10       Impact factor: 4.177

5.  Comparative evaluation of NASBA HIV-1 RNA QT, AMPLICOR-HIV monitor, and QUANTIPLEX HIV RNA assay, three methods for quantification of human immunodeficiency virus type 1 RNA in plasma.

Authors:  H Revets; D Marissens; S de Wit; P Lacor; N Clumeck; S Lauwers; G Zissis
Journal:  J Clin Microbiol       Date:  1996-05       Impact factor: 5.948

6.  Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

Authors:  P Chomczynski; N Sacchi
Journal:  Anal Biochem       Date:  1987-04       Impact factor: 3.365

7.  Quantitation of plasma human immunodeficiency virus type 1 RNA by competitive polymerase chain reaction.

Authors:  D T Scadden; Z Wang; J E Groopman
Journal:  J Infect Dis       Date:  1992-06       Impact factor: 5.226

8.  A one-tube quantitative HIV-1 RNA NASBA nucleic acid amplification assay using electrochemiluminescent (ECL) labelled probes.

Authors:  B van Gemen; R van Beuningen; A Nabbe; D van Strijp; S Jurriaans; P Lens; T Kievits
Journal:  J Virol Methods       Date:  1994-09       Impact factor: 2.014

9.  Rapid and simple PCR assay for quantitation of human immunodeficiency virus type 1 RNA in plasma: application to acute retroviral infection.

Authors:  J Mulder; N McKinney; C Christopherson; J Sninsky; L Greenfield; S Kwok
Journal:  J Clin Microbiol       Date:  1994-02       Impact factor: 5.948

10.  High levels of HIV-1 in plasma during all stages of infection determined by competitive PCR.

Authors:  M Piatak; M S Saag; L C Yang; S J Clark; J C Kappes; K C Luk; B H Hahn; G M Shaw; J D Lifson
Journal:  Science       Date:  1993-03-19       Impact factor: 47.728
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  1 in total

1.  Clinical evaluation of an in-house reverse transcription-competitive PCR for quantitation of human immunodeficiency virus type 1 RNA in plasma.

Authors:  M Zazzi; L Romano; M Catucci; G Venturi; A De Milito; P E Valensin
Journal:  J Clin Microbiol       Date:  1999-02       Impact factor: 5.948
  1 in total

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