Literature DB >> 1250699

Thermostable polynucleotide phosphorylases from Bacillus stearothermophilus and Thermus aquaticus.

J N Wood, D W Hutchinson.   

Abstract

Polynucleotide phosphorylase from Bacillus stearothermophilus has been purified to homogeneity. Polyacrylamide gel electrophoresis run under denaturing conditions indicates that the enzyme is a tetramer with subunits of apparent molecular weight 51,000 daltons. A partial purification of polynucleotide phosphorylase from Thermus aquaticus has also been effected. The two enzymes show similar catalytic properties, which differ little from those of mesophilic polynucleotide phosphorylases. The use of thermostable polynucleotide phosphorylases for in vitro nucleic acid synthesis is discussed.

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Year:  1976        PMID： 1250699      PMCID： PMC342889          DOI： 10.1093/nar/3.1.219

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  13 in total

1.  Further characterization of the polynucleotide phosphorylase of Micrococcus luteus.

Authors:  C H Letendre; M F Singer
Journal:  Nucleic Acids Res       Date:  1975-02       Impact factor: 16.971

2.  High resolution two-dimensional electrophoresis of proteins.

Authors:  P H O'Farrell
Journal:  J Biol Chem       Date:  1975-05-25       Impact factor: 5.157

3.  Quaternary structure of polynucleotide phosphorylase from Escherichia coli.

Authors:  C Portier; R van Rapenbusch; M Grunberg-Manago
Journal:  Eur J Biochem       Date:  1973-12-03

4.  Conformation of oligoinosinates: chain-length dependence and comparison to other oligonucleotides.

Authors:  S Tazawa; I Tazawa; J L Alderfer; P O Ts'o
Journal:  Biochemistry       Date:  1972-09-12       Impact factor: 3.162

5.  Amino-terminal sequence analysis of proteins purified on a nanomole scale by gel electrophoresis.

Authors:  A M Weiner; T Platt; K Weber
Journal:  J Biol Chem       Date:  1972-05-25       Impact factor: 5.157

6.  Studies on an ATPase of thermophilic bacteria. I. Purification and properties.

Authors:  A Hachimori; N Muramatsu; Y Noso
Journal:  Biochim Biophys Acta       Date:  1970-06-10

7.  The relationship of mono- and polynucleotide conformation to catalysis by polynucleotide phosphorylase.

Authors:  A M Kapuler; C Monny; A M Michelson
Journal:  Biochim Biophys Acta       Date:  1970-09-17

8.  Manganese accumulation by Escherichia coli: evidence for a specific transport system.

Authors:  S Silver; M L Kralovic
Journal:  Biochem Biophys Res Commun       Date:  1969-03-10       Impact factor: 3.575

9.  Measurement of molecular weights by electrophoresis on SDS-acrylamide gel.

Authors:  K Weber; J R Pringle; M Osborn
Journal:  Methods Enzymol       Date:  1972       Impact factor: 1.600

10.  Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile.

Authors:  T D Brock; H Freeze
Journal:  J Bacteriol       Date:  1969-04       Impact factor: 3.490
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  3 in total

1.  A simple, rapid preparation of alpha[32P]-labelled adenosine diphosphate.

Authors:  I L Cartwright; D W Hutchinson
Journal:  Nucleic Acids Res       Date:  1977-07       Impact factor: 16.971

2.  RhlB helicase rather than enolase is the beta-subunit of the Escherichia coli polynucleotide phosphorylase (PNPase)-exoribonucleolytic complex.

Authors:  Pei-Hsun Lin; Sue Lin-Chao
Journal:  Proc Natl Acad Sci U S A       Date:  2005-11-07       Impact factor: 11.205

3.  Purification of an Endogenous polynucleotide phosphorylase from Brevibacterium JM98A.

Authors:  H H Yang; D W Thayer; S P Yang
Journal:  Appl Environ Microbiol       Date:  1979-07       Impact factor: 4.792
  3 in total

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