OBJECTIVES: To compare the sensitivity of four rapid assays for the detection of antibodies against HIV-1 during early seroconversion. METHODS: Four rapid assays for the detection of antibodies to HIV-1/HIV-2 (SUDS HIV 1 + 2, TestPack HIV-1/HIV-2, HIV-SPOT and CAPILLUS HIV-1/HIV-2) were evaluated on 38 sera derived from 14 HIV-1 seroconverters, formerly tested with Abbott second- and third-generation HIV-1/HIV-2 enzyme immunoassay (EIA) and Diagnostic Biotechnology HIV blot 2.2. EIA-negative sera had also been investigated by HIV-1 antigen testing and polymerase chain reaction (PCR) analysis detecting HIV-1 proviral DNA. RESULTS: On 16 sera which were Abbott second-generation EIA negative, the SUDS assay rated highest with six positive results. Four sera were TestPack positive. No specimens in this group were HIV-SPOT positive or CAPILLUS positive. Of the remaining 22 sera (Abbott second- and third-generation EIA positive), all but two were positive in all tests. CONCLUSIONS: The study revealed differences in the sensitivities of the rapid assays in the early phase of seroconversion. The most sensitive assay, SUDS, was even more sensitive than the Abbott third-generation EIA, while TestPack and the EIA were equally sensitive. Based on these findings, we suggest the inclusion of these assays in the supplemental testing for detection of antibodies to HIV.
OBJECTIVES: To compare the sensitivity of four rapid assays for the detection of antibodies against HIV-1 during early seroconversion. METHODS: Four rapid assays for the detection of antibodies to HIV-1/HIV-2 (SUDS HIV 1 + 2, TestPack HIV-1/HIV-2, HIV-SPOT and CAPILLUS HIV-1/HIV-2) were evaluated on 38 sera derived from 14 HIV-1 seroconverters, formerly tested with Abbott second- and third-generation HIV-1/HIV-2 enzyme immunoassay (EIA) and Diagnostic Biotechnology HIV blot 2.2. EIA-negative sera had also been investigated by HIV-1 antigen testing and polymerase chain reaction (PCR) analysis detecting HIV-1 proviral DNA. RESULTS: On 16 sera which were Abbott second-generation EIA negative, the SUDS assay rated highest with six positive results. Four sera were TestPack positive. No specimens in this group were HIV-SPOT positive or CAPILLUS positive. Of the remaining 22 sera (Abbott second- and third-generation EIA positive), all but two were positive in all tests. CONCLUSIONS: The study revealed differences in the sensitivities of the rapid assays in the early phase of seroconversion. The most sensitive assay, SUDS, was even more sensitive than the Abbott third-generation EIA, while TestPack and the EIA were equally sensitive. Based on these findings, we suggest the inclusion of these assays in the supplemental testing for detection of antibodies to HIV.
Authors: Joshua D O'Neal; Matthew R Golden; Bernard M Branson; Joanne D Stekler Journal: J Acquir Immune Defic Syndr Date: 2012-08-01 Impact factor: 3.731
Authors: Lauren R Violette; Andy Cornelius-Hudson; Madison Snidarich; Lisa A Niemann; Sonny Michael Assennato; Allyson Ritchie; Neha Goel; Pollyanna R Chavez; Steven F Ethridge; David A Katz; Helen Lee; Kevin P Delaney; Joanne D Stekler Journal: J Acquir Immune Defic Syndr Date: 2022-04-15 Impact factor: 3.771
Authors: Joanne D Stekler; Paul D Swenson; Robert W Coombs; Joan Dragavon; Katherine K Thomas; Catherine A Brennan; Sushil G Devare; Robert W Wood; Matthew R Golden Journal: Clin Infect Dis Date: 2009-08-01 Impact factor: 9.079
Authors: Brian Louie; Ernest Wong; Jeffrey D Klausner; Sally Liska; Frederick Hecht; Terri Dowling; Martha Obeso; Susan S Phillips; Mark W Pandori Journal: J Clin Microbiol Date: 2008-01-30 Impact factor: 5.948
Authors: Ann M Buchanan; Behzad Nadjm; Ben Amos; George Mtove; David Sifuna; Coleen K Cunningham; John A Crump; Hugh Reyburn Journal: J Clin Virol Date: 2012-08-24 Impact factor: 3.168
Authors: Meghan K Mayhood; Isaac A Afwamba; Christopher O Odhiambo; Epimack Ndanu; Nathan M Thielman; Anne B Morrissey; John F Shao; Brian Wells Pence; John A Crump Journal: J Clin Microbiol Date: 2008-10-15 Impact factor: 5.948
Authors: Joanne D Stekler; Joshua D O'Neal; Aric Lane; Fred Swanson; Janine Maenza; Claire E Stevens; Robert W Coombs; Joan A Dragavon; Paul D Swenson; Matthew R Golden; Bernard M Branson Journal: J Clin Virol Date: 2013-12 Impact factor: 3.168