Literature DB >> 9041390

PCR identification of Mycobacterium bovis BCG.

E A Talbot1, D L Williams, R Frothingham.   

Abstract

The attenuated bacillus Calmette-Guérin (BCG) vaccine strain is derived from a virulent strain of Mycobacterium bovis. BCG is difficult to differentiate from other strains of M. bovis and other members of the M. tuberculosis complex by conventional methods. Recently, a genomic region designated RD1 was found to be present in all virulent M. bovis and M. tuberculosis strains tested but deleted from all BCG strains tested. With this information, a multiplex PCR method was developed to detect the RD1 deletion. A large collection of BCG and other M. tuberculosis complex strains from diverse host and geographic origins was tested. RD1 was deleted in 23 of 23 BCG strains. RD1 was present in 129 of 129 other M. tuberculosis complex strains. This multiplex PCR method can be used as a tool for the rapid and specific identification of BCG.

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Year:  1997        PMID： 9041390      PMCID： PMC229628          DOI： 10.1128/jcm.35.3.566-569.1997

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  28 in total

1.  Use of gene probes based on the insertion sequence IS986 to differentiate between BCG vaccine strains.

Authors:  N G Fomukong; J W Dale; T W Osborn; J M Grange
Journal:  J Appl Bacteriol       Date:  1992-02

Review 2.  Possible hazards of routine bacillus Calmette-Guérin immunization in human immunodeficiency virus-infected children.

Authors:  K M Edwards; D S Kernodle
Journal:  Pediatr Infect Dis J       Date:  1996-09       Impact factor: 2.129

Review 3.  Second IUATLD study on complications induced by intradermal BCG-vaccination.

Authors:  A Lotte; O Wasz-Hockert; N Poisson; H Engbaek; H Landmann; U Quast; B Andrasofszky; L Lugosi; I Vadasz; P Mihailescu
Journal:  Bull Int Union Tuberc Lung Dis       Date:  1988-06

4.  Separation of Mycobacterium bovis BCG from Mycobacterium tuberculosis and Mycobacterium bovis by using high-performance liquid chromatography of mycolic acids.

Authors:  M M Floyd; V A Silcox; W D Jones; W R Butler; J O Kilburn
Journal:  J Clin Microbiol       Date:  1992-05       Impact factor: 5.948

5.  IS6110: conservation of sequence in the Mycobacterium tuberculosis complex and its utilization in DNA fingerprinting.

Authors:  M D Cave; K D Eisenach; P F McDermott; J H Bates; J T Crawford
Journal:  Mol Cell Probes       Date:  1991-02       Impact factor: 2.365

6.  BCG complications. Estimates of the risks among vaccinated subjects and statistical analysis of their main characteristics.

Authors:  A Lotte; O Wasz-Höckert; N Poisson; N Dumitrescu; M Verron; E Couvet
Journal:  Adv Tuberc Res       Date:  1984

7.  Differentiation of known strains of BCG from isolates of mycobacterium bovis and Mycobacterium tuberculosis by using mycobacteriophage 33D.

Authors:  W D Jones
Journal:  J Clin Microbiol       Date:  1975-04       Impact factor: 5.948

8.  Isolation, characterization, and molecular cloning of a specific Mycobacterium tuberculosis antigen gene: identification of a species-specific sequence.

Authors:  C A Parra; L P Londoño; P Del Portillo; M E Patarroyo
Journal:  Infect Immun       Date:  1991-10       Impact factor: 3.441

9.  Subdivision of daughter strains of bacille Calmette-Guérin (BCG) according to secreted protein patterns.

Authors:  C Abou-Zeid; I Smith; J Grange; J Steele; G Rook
Journal:  J Gen Microbiol       Date:  1986-11

10.  Incidence and treatment of complications of bacillus Calmette-Guerin intravesical therapy in superficial bladder cancer.

Authors:  D L Lamm; P M van der Meijden; A Morales; S A Brosman; W J Catalona; H W Herr; M S Soloway; A Steg; F M Debruyne
Journal:  J Urol       Date:  1992-03       Impact factor: 7.450
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  55 in total

1.  Rapid testing for nitrate reductase activity of Mycobacterium tuberculosis grown in an automated culture system.

Authors:  A Meyer; L Sedlacek; F-C Bange
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2003-06-24       Impact factor: 3.267

2.  Evaluation of genotype and LiPA MYCOBACTERIA assays for identification of Finnish mycobacterial isolates.

Authors:  Johanna Mäkinen; Aleksi Sarkola; Merja Marjamäki; Matti K Viljanen; Hanna Soini
Journal:  J Clin Microbiol       Date:  2002-09       Impact factor: 5.948

3.  Immune reconstitution syndrome after highly active antiretroviral therapy in human immunodeficiency virus-infected thai children.

Authors:  Thanyawee Puthanakit; Peninnah Oberdorfer; Noppadon Akarathum; Pornphun Wannarit; Thira Sirisanthana; Virat Sirisanthana
Journal:  Pediatr Infect Dis J       Date:  2006-01       Impact factor: 2.129

4.  Comparison of GenoType MTBC with RFLP-PCR and multiplex PCR to identify Mycobacterium tuberculosis complex species.

Authors:  M P Romero Gómez; L Herrera-León; M S Jiménez; J García Rodríguez
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2007-01       Impact factor: 3.267

5.  Evaluation of the invader assay with the BACTEC MGIT 960 system for prompt isolation and identification of mycobacterial species from clinical specimens.

Authors:  Sadahiro Ichimura; Makoto Nagano; Nobuko Ito; Masahiro Shimojima; Toru Egashira; Chikara Miyamoto; Kiyofumi Ohkusu; Takayuki Ezaki
Journal:  J Clin Microbiol       Date:  2007-08-08       Impact factor: 5.948

6.  PCR-based method to differentiate the subspecies of the Mycobacterium tuberculosis complex on the basis of genomic deletions.

Authors:  Richard C Huard; Luiz Claudio de Oliveira Lazzarini; W Ray Butler; Dick van Soolingen; John L Ho
Journal:  J Clin Microbiol       Date:  2003-04       Impact factor: 5.948

7.  Polymorphic nucleotide within the promoter of nitrate reductase (NarGHJI) is specific for Mycobacterium tuberculosis.

Authors:  Marion Stermann; Antje Bohrssen; Catharina Diephaus; Silvia Maass; Franz-Christoph Bange
Journal:  J Clin Microbiol       Date:  2003-07       Impact factor: 5.948

8.  Bacterial artificial chromosome-based comparative genomic analysis identifies Mycobacterium microti as a natural ESAT-6 deletion mutant.

Authors:  Priscille Brodin; Karin Eiglmeier; Magali Marmiesse; Alain Billault; Thierry Garnier; Stefan Niemann; Stewart T Cole; Roland Brosch
Journal:  Infect Immun       Date:  2002-10       Impact factor: 3.441

9.  Specific differentiation between Mycobacterium bovis BCG and virulent strains of the Mycobacterium tuberculosis complex.

Authors:  J Magdalena; P Supply; C Locht
Journal:  J Clin Microbiol       Date:  1998-09       Impact factor: 5.948

10.  Frequency of Mycobacterium bovis as an etiologic agent in extrapulmonary tuberculosis in HIV-positive and -negative Mexican patients.

Authors:  R Cicero; H Olivera; A Hernández-Solis; E Ramírez-Casanova; A Escobar-Gutiérrez
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2008-11-04       Impact factor: 3.267
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