Literature DB >> 1583141

Separation of Mycobacterium bovis BCG from Mycobacterium tuberculosis and Mycobacterium bovis by using high-performance liquid chromatography of mycolic acids.

M M Floyd1, V A Silcox, W D Jones, W R Butler, J O Kilburn.   

Abstract

Profile analysis of mycolic acid ester patterns of Mycobacterium tuberculosis, Mycobacterium bovis, and Mycobacterium bovis bacillus Calmette-Gúerin (BCG) using high-performance liquid chromatography indicated that separation of BCG from M. tuberculosis and M. bovis by elution and relative retention times is possible. Mycolic acid patterns of BCG eluted from the column 0.5 min before M. tuberculosis or M. bovis, resulting in relative retention times for two peaks not seen in the pattern of M. tuberculosis or M. bovis. Identification was confirmed by phage typing, which has been the standard procedure for confirmation of BCG strains. These results showed that high-performance liquid chromatographic analysis of mycolic acid esters can be used in the mycobacterial reference laboratory for separation of BCG from M. tuberculosis and M. bovis.

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Year:  1992        PMID： 1583141      PMCID： PMC265278          DOI： 10.1128/jcm.30.5.1327-1330.1992

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  9 in total

1.  Further studies of mycobacteriophage 33D (Warsaw) for differentiation of BCG from M. bovis and M. tubeculosis.

Authors:  W D Jones
Journal:  Tubercle       Date:  1979-03

2.  Identification of mycobacteria by high-performance liquid chromatography.

Authors:  W R Butler; K C Jost; J O Kilburn
Journal:  J Clin Microbiol       Date:  1991-11       Impact factor: 5.948

3.  Differentiation of BCG from other variants of Mycobacterium tuberculosis isolated from clinical material.

Authors:  M D Yates; C H Collins; J M Grange
Journal:  Tubercle       Date:  1978-06

4.  BCG identification by DNA restriction fragment patterns.

Authors:  D M Collins; G W De Lisle
Journal:  J Gen Microbiol       Date:  1987-06

Review 5.  History of BCG and its substrains.

Authors:  R Crispen
Journal:  Prog Clin Biol Res       Date:  1989

6.  Identification of an effective vaccine against tuberculosis.

Authors:  G W Comstock
Journal:  Am Rev Respir Dis       Date:  1988-08

7.  High-performance liquid chromatography patterns of mycolic acids as criteria for identification of Mycobacterium chelonae, Mycobacterium fortuitum, and Mycobacterium smegmatis.

Authors:  W R Butler; J O Kilburn
Journal:  J Clin Microbiol       Date:  1990-09       Impact factor: 5.948

8.  Differentiation of known strains of BCG from isolates of mycobacterium bovis and Mycobacterium tuberculosis by using mycobacteriophage 33D.

Authors:  W D Jones
Journal:  J Clin Microbiol       Date:  1975-04       Impact factor: 5.948

9.  Identification of major slowly growing pathogenic mycobacteria and Mycobacterium gordonae by high-performance liquid chromatography of their mycolic acids.

Authors:  W R Butler; J O Kilburn
Journal:  J Clin Microbiol       Date:  1988-01       Impact factor: 5.948
  9 in total
  32 in total

1.  PCR identification of Mycobacterium bovis BCG.

Authors:  E A Talbot; D L Williams; R Frothingham
Journal:  J Clin Microbiol       Date:  1997-03       Impact factor: 5.948

2.  A case of Pott's disease with epidural abscess and probable cerebral tuberculoma following Bacillus Calmette-Guérin therapy for superficial bladder cancer.

Authors:  Colin B Josephson; Saleh Al-Azri; Daniel J Smyth; David Haase; B Lynn Johnston
Journal:  Can J Infect Dis Med Microbiol       Date:  2010       Impact factor: 2.471

3.  Genetic identification of Mycobacterium bovis BCG by restriction fragment length polymorphism analysis of the direct-repeat region.

Authors:  S T Howard; A Laszlo; W M Johnson
Journal:  J Clin Microbiol       Date:  1997-04       Impact factor: 5.948

4.  Application of the Sherlock Mycobacteria Identification System using high-performance liquid chromatography in a clinical laboratory.

Authors:  J A Kellogg; D A Bankert; G S Withers; W Sweimler; T E Kiehn; G E Pfyffer
Journal:  J Clin Microbiol       Date:  2001-03       Impact factor: 5.948

5.  Identification of a polymorphic nucleotide in oxyR specific for Mycobacterium bovis.

Authors:  S Sreevatsan; P Escalante; X Pan; D A Gillies; S Siddiqui; C N Khalaf; B N Kreiswirth; P Bifani; L G Adams; T Ficht; V S Perumaalla; M D Cave; J D van Embden; J M Musser
Journal:  J Clin Microbiol       Date:  1996-08       Impact factor: 5.948

6.  Specific differentiation between Mycobacterium bovis BCG and virulent strains of the Mycobacterium tuberculosis complex.

Authors:  J Magdalena; P Supply; C Locht
Journal:  J Clin Microbiol       Date:  1998-09       Impact factor: 5.948

7.  Clinical significance, biochemical features, and susceptibility patterns of sporadic isolates of the Mycobacterium chelonae-like organism.

Authors:  R J Wallace; V A Silcox; M Tsukamura; B A Brown; J O Kilburn; W R Butler; G Onyi
Journal:  J Clin Microbiol       Date:  1993-12       Impact factor: 5.948

8.  Rapid identification of mycolic acid patterns of mycobacteria by high-performance liquid chromatography using pattern recognition software and a Mycobacterium library.

Authors:  S E Glickman; J O Kilburn; W R Butler; L S Ramos
Journal:  J Clin Microbiol       Date:  1994-03       Impact factor: 5.948

9.  Mycobacterium bovis BCG vertebral osteomyelitis after intravesical BCG therapy, diagnosed by PCR-based genomic deletion analysis.

Authors:  Takuya Nikaido; Kei Ishibashi; Koji Otani; Shoji Yabuki; Shinichi Konno; Shuichi Mori; Kazutaka Ohashi; Takashi Ishida; Michiko Nakano; Osamu Yamaguchi; Tatsuo Suzutani; Shinichi Kikuchi
Journal:  J Clin Microbiol       Date:  2007-10-24       Impact factor: 5.948

Review 10.  The new diagnostic mycobacteriology laboratory.

Authors:  M Salfinger; G E Pfyffer
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1994-11       Impact factor: 3.267
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