Literature DB >> 9013869

Solubility diagram of the Rhodobacter sphaeroides reaction center as a function of PEG concentration.

J F Gaucher1, M Riès-Kautt, F Reiss-Husson, A Ducruix.   

Abstract

In order to quantify the effect of polyethylene glycol 4000 (PEG) on the solubility of an integral membrane protein, we have crystallized the photochemical reaction center from Rhodobacter sphaeroides Y by batch method on a large range of PEG. The measurement of the solubility diagram display a semi-logarithmic dependence of solubility versus PEG concentration. Comparison of our results with previously published ones [Odahara, T., Ataka, M. and Katsura, M. (1994) Acta Cryst. D50, 639-642] suggests a notable effect of additional 1,2,3-heptane-triol and/or temperature on photochemical reaction center solubility.

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Year:  1997        PMID： 9013869     DOI： 10.1016/s0014-5793(96)01446-9

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  5 in total

1.  Phase knowledge enables rational screens for protein crystallization.

Authors:  Megan J Anderson; Carl L Hansen; Stephen R Quake
Journal:  Proc Natl Acad Sci U S A       Date:  2006-10-30       Impact factor: 11.205

2.  Precipitation diagram and optimization of crystallization conditions at low ionic strength for deglycosylated dye-decolorizing peroxidase from a basidiomycete.

Authors:  Shinya Saijo; Takao Sato; Nobuo Tanaka; Atsushi Ichiyanagi; Yasushi Sugano; Makoto Shoda
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2005-07-08

3.  Determination of the phase diagram for soluble and membrane proteins.

Authors:  Sameer Talreja; Sarah L Perry; Sudipto Guha; Venkateswarlu Bhamidi; Charles F Zukoski; Paul J A Kenis
Journal:  J Phys Chem B       Date:  2010-04-08       Impact factor: 2.991

4.  Solubility of lysozyme in polyethylene glycol-electrolyte mixtures: the depletion interaction and ion-specific effects.

Authors:  Matjaz Boncina; Jurij Rescic; Vojko Vlachy
Journal:  Biophys J       Date:  2008-04-25       Impact factor: 4.033

Review 5.  Crystallization screening: the influence of history on current practice.

Authors:  Joseph R Luft; Janet Newman; Edward H Snell
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2014-06-27       Impact factor: 1.056
  5 in total

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