Literature DB >> 9003635

Quantitation of cytomegalovirus (CMV) DNA in leukocytes of human immunodeficiency virus-infected subjects with and without CMV disease by using PCR and the SHARP Signal Detection System.

G Boivin1, J Handfield, G Murray, E Toma, R Lalonde, J G Lazar, M G Bergeron.   

Abstract

We report the development of a simple and rapid PCR assay for quantitation of the cytomegalovirus (CMV) DNA load in polymorphonuclear leukocytes. Using this system, a very good correlation was found between a high number of CMV copies in the blood and the presence of CMV disease in subjects with AIDS.

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Year:  1997        PMID： 9003635      PMCID： PMC229619          DOI： 10.1128/jcm.35.2.525-526.1997

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  14 in total

1.  Detection of cytomegalovirus in blood donors by PCR using the digene SHARP signal system assay: effects of sample preparation and detection methodology.

Authors:  M Krajden; P Shankaran; C Bourke; W Lau
Journal:  J Clin Microbiol       Date:  1996-01       Impact factor: 5.948

2.  Comparison of PCR and pp65 antigenemia assay with quantitative shell vial culture for detection of cytomegalovirus in blood leukocytes from solid-organ transplant recipients.

Authors:  G A Storch; R S Buller; T C Bailey; N A Ettinger; T Langlois; M Gaudreault-Keener; P L Welby
Journal:  J Clin Microbiol       Date:  1994-04       Impact factor: 5.948

3.  High levels of circulating cytomegalovirus DNA reflect visceral organ disease in viremic immunosuppressed patients other than marrow recipients.

Authors:  R L Saltzman; M R Quirk; M C Jordan
Journal:  J Clin Invest       Date:  1992-11       Impact factor: 14.808

4.  Evaluation of SHARP signal system for enzymatic detection of amplified hepatitis B virus DNA.

Authors:  E Valentine-Thon
Journal:  J Clin Microbiol       Date:  1995-02       Impact factor: 5.948

5.  Quantification of human cytomegalovirus DNA in peripheral blood polymorphonuclear leukocytes of immunocompromised patients by the polymerase chain reaction.

Authors:  D Zipeto; F Baldanti; D Zella; M Furione; A Cavicchini; G Milanesi; G Gerna
Journal:  J Virol Methods       Date:  1993-09       Impact factor: 2.014

6.  Monitoring of human cytomegalovirus infections and ganciclovir treatment in heart transplant recipients by determination of viremia, antigenemia, and DNAemia.

Authors:  G Gerna; D Zipeto; M Parea; M G Revello; E Silini; E Percivalle; M Zavattoni; P Grossi; G Milanesi
Journal:  J Infect Dis       Date:  1991-09       Impact factor: 5.226

7.  Quantification of human cytomegalovirus DNA using the polymerase chain reaction.

Authors:  J C Fox; P D Griffiths; V C Emery
Journal:  J Gen Virol       Date:  1992-09       Impact factor: 3.891

8.  Detection of cytomegalovirus (CMV) in granulocytes by polymerase chain reaction compared with the CMV antigen test.

Authors:  G J Boland; R A de Weger; M G Tilanus; C Ververs; K Bosboom-Kalsbeek; G C de Gast
Journal:  J Clin Microbiol       Date:  1992-07       Impact factor: 5.948

9.  Development and clinical significance of a diagnostic assay based on the polymerase chain reaction for detection of human cytomegalovirus DNA in blood samples from immunocompromised patients.

Authors:  D Zipeto; M G Revello; E Silini; M Parea; E Percivalle; M Zavattoni; G Milanesi; G Gerna
Journal:  J Clin Microbiol       Date:  1992-02       Impact factor: 5.948

10.  Quantitation of human cytomegalovirus DNA from peripheral blood cells of human immunodeficiency virus-infected patients could predict cytomegalovirus retinitis.

Authors:  L Rasmussen; S Morris; D Zipeto; J Fessel; R Wolitz; A Dowling; T C Merigan
Journal:  J Infect Dis       Date:  1995-01       Impact factor: 5.226
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  8 in total

Review 1.  Real-time PCR in virology.

Authors:  Ian M Mackay; Katherine E Arden; Andreas Nitsche
Journal:  Nucleic Acids Res       Date:  2002-03-15       Impact factor: 16.971

2.  Evaluation of the AMPLICOR cytomegalovirus test with specimens from human immunodeficiency virus-infected subjects.

Authors:  G Boivin; J Handfield; E Toma; G Murray; R Lalonde; V J Tevere; R Sun; M G Bergeron
Journal:  J Clin Microbiol       Date:  1998-09       Impact factor: 5.948

3.  New reporter cell line to evaluate the sequential emergence of multiple human cytomegalovirus mutations during in vitro drug exposure.

Authors:  C Gilbert; G Boivin
Journal:  Antimicrob Agents Chemother       Date:  2005-12       Impact factor: 5.191

4.  Detection of human cytomegalovirus pp67 late gene transcripts in cerebrospinal fluid of human immunodeficiency virus type 1-infected patients by nucleic acid sequence-based amplification.

Authors:  F Zhang; S Tetali; X P Wang; M H Kaplan; F V Cromme; C C Ginocchio
Journal:  J Clin Microbiol       Date:  2000-05       Impact factor: 5.948

5.  Assessment of antigenemia assay for the diagnosis of cytomegalovirus gastrointestinal diseases in HIV-infected patients.

Authors:  Yohei Hamada; Naoyoshi Nagata; Takuro Shimbo; Toru Igari; Ryo Nakashima; Naoki Asayama; So Nishimura; Hirohisa Yazaki; Katsuji Teruya; Hiroyuki Gatanaga; Yoshimi Kikuchi; Junichi Akiyama; Norio Ohmagari; Naomi Uemura; Shinichi Oka
Journal:  AIDS Patient Care STDS       Date:  2013-06-25       Impact factor: 5.078

6.  Long-term predictive value of a single cytomegalovirus (CMV) DNA PCR assay for CMV disease in human immunodeficiency virus-infected patients.

Authors:  S Walmsley; T Mazzulli; M Krajden
Journal:  J Clin Microbiol       Date:  1998-01       Impact factor: 5.948

7.  Human herpesvirus 8 DNA load in leukocytes of human immunodeficiency virus-infected subjects: correlation with the presence of Kaposi's sarcoma and response to anticytomegalovirus therapy.

Authors:  G Boivin; A Gaudreau; E Toma; R Lalonde; J P Routy; G Murray; J Handfield; M G Bergeron
Journal:  Antimicrob Agents Chemother       Date:  1999-02       Impact factor: 5.191

Review 8.  Quantitation of cytomegalovirus: methodologic aspects and clinical applications.

Authors:  M Boeckh; G Boivin
Journal:  Clin Microbiol Rev       Date:  1998-07       Impact factor: 26.132
  8 in total

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