Literature DB >> 1311338

Development and clinical significance of a diagnostic assay based on the polymerase chain reaction for detection of human cytomegalovirus DNA in blood samples from immunocompromised patients.

D Zipeto1, M G Revello, E Silini, M Parea, E Percivalle, M Zavattoni, G Milanesi, G Gerna.   

Abstract

The presence of human cytomegalovirus (HCMV) DNA in blood was investigated by the polymerase chain reaction (PCR) in 293 blood samples from 86 immunocompromised patients. Of the 86 patients, 23 underwent clinical and virologic follow-up for HCMV infection. In parallel, blood samples were examined for viremia and antigenemia. Concordant results between PCR and assays for viremia and antigenemia were obtained on 124 positive and 110 negative samples, with an overall concordance of 79.8%, while 59 samples (most from patients with HCMV infection) were positive by PCR alone. PCR is a new powerful tool for detection of HCMV infections in blood samples from immunocompromised patients. However, its clinical significance appears to be restricted to the indication of a risk of reactivation of HCMV infection.

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Year:  1992        PMID： 1311338      PMCID： PMC265096          DOI： 10.1128/jcm.30.2.527-530.1992

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  18 in total

1.  Disseminated cytomegalovirus infection. Molecular analysis of virus and leukocyte interactions in viremia.

Authors:  R L Saltzman; M R Quirk; M C Jordan
Journal:  J Clin Invest       Date:  1988-01       Impact factor: 14.808

2.  The structure of the major immediate early gene of human cytomegalovirus strain AD169.

Authors:  A Akrigg; G W Wilkinson; J D Oram
Journal:  Virus Res       Date:  1985-03       Impact factor: 3.303

3.  Avoiding false positives with PCR.

Authors:  S Kwok; R Higuchi
Journal:  Nature       Date:  1989-05-18       Impact factor: 49.962

4.  A potent inhibitor of Taq polymerase copurifies with human genomic DNA.

Authors:  R de Franchis; N C Cross; N S Foulkes; T M Cox
Journal:  Nucleic Acids Res       Date:  1988-11-11       Impact factor: 16.971

5.  Structural analysis of the major immediate early gene of human cytomegalovirus.

Authors:  R M Stenberg; D R Thomsen; M F Stinski
Journal:  J Virol       Date:  1984-01       Impact factor: 5.103

6.  The two major structural phosphoproteins (pp65 and pp150) of human cytomegalovirus and their antigenic properties.

Authors:  G Jahn; B C Scholl; B Traupe; B Fleckenstein
Journal:  J Gen Virol       Date:  1987-05       Impact factor: 3.891

7.  Detection of cytomegalovirus DNA in peripheral blood of patients infected with human immunodeficiency virus.

Authors:  D Shibata; W J Martin; M D Appleman; D M Causey; J M Leedom; N Arnheim
Journal:  J Infect Dis       Date:  1988-12       Impact factor: 5.226

8.  Enzymatic amplification of human cytomegalovirus sequences by polymerase chain reaction.

Authors:  K Hsia; D H Spector; J Lawrie; S A Spector
Journal:  J Clin Microbiol       Date:  1989-08       Impact factor: 5.948

9.  Detection of human cytomegalovirus in clinical specimens by DNA-DNA hybridization.

Authors:  S A Spector; J A Rua; D H Spector; R McMillan
Journal:  J Infect Dis       Date:  1984-07       Impact factor: 5.226

10.  Identification of human cytomegalovirus isolates by the polymerase chain reaction.

Authors:  D Zipeto; E Silini; M Parea; E Percivalle; M Zavattoni; A Di Matteo; G Milanesi; G Gerna
Journal:  Microbiologica       Date:  1990-10
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  19 in total

1.  Development of a quadriplex polymerase chain reaction for human cytomegalovirus detection.

Authors:  P Markoulatos; V Samara; N Siafakas; E Plakokefalos; N Spyrou; M L Moncany
Journal:  J Clin Lab Anal       Date:  1999       Impact factor: 2.352

2.  Quantitation of cytomegalovirus (CMV) DNA in leukocytes of human immunodeficiency virus-infected subjects with and without CMV disease by using PCR and the SHARP Signal Detection System.

Authors:  G Boivin; J Handfield; G Murray; E Toma; R Lalonde; J G Lazar; M G Bergeron
Journal:  J Clin Microbiol       Date:  1997-02       Impact factor: 5.948

3.  Evaluation of the Digene Hybrid Capture System for detection and quantitation of human cytomegalovirus viremia in human immunodeficiency virus-infected patients.

Authors:  T Mazzulli; S Wood; R Chua; S Walmsley
Journal:  J Clin Microbiol       Date:  1996-12       Impact factor: 5.948

Review 4.  Management of cytomegalovirus infection after solid-organ or stem-cell transplantation. Current guidelines and future prospects.

Authors:  H Hebart; L Kanz; G Jahn; H Einsele
Journal:  Drugs       Date:  1998-01       Impact factor: 9.546

Review 5.  New strategies for prevention and therapy of cytomegalovirus infection and disease in solid-organ transplant recipients.

Authors:  I G Sia; R Patel
Journal:  Clin Microbiol Rev       Date:  2000-01       Impact factor: 26.132

6.  Single amino acid changes in the DNA polymerase confer foscarnet resistance and slow-growth phenotype, while mutations in the UL97-encoded phosphotransferase confer ganciclovir resistance in three double-resistant human cytomegalovirus strains recovered from patients with AIDS.

Authors:  F Baldanti; M R Underwood; S C Stanat; K K Biron; S Chou; A Sarasini; E Silini; G Gerna
Journal:  J Virol       Date:  1996-03       Impact factor: 5.103

7.  Human cytomegalovirus immediate-early mRNA detection by nucleic acid sequence-based amplification as a new parameter for preemptive therapy in bone marrow transplant recipients.

Authors:  G Gerna; F Baldanti; D Lilleri; M Parea; E Alessandrino; A Pagani; F Locatelli; J Middeldorp; M G Revello
Journal:  J Clin Microbiol       Date:  2000-05       Impact factor: 5.948

8.  Human cytomegalovirus (HCMV) late-mRNA detection in peripheral blood of AIDS patients: diagnostic value for HCMV disease compared with those of viral culture and HCMV DNA detection.

Authors:  J Gozlan; J M Salord; C Chouaïd; C Duvivier; O Picard; M C Meyohas; J C Petit
Journal:  J Clin Microbiol       Date:  1993-07       Impact factor: 5.948

9.  Comparative quantitation of human cytomegalovirus DNA in blood leukocytes and plasma of transplant and AIDS patients.

Authors:  G Gerna; M Furione; F Baldanti; A Sarasini
Journal:  J Clin Microbiol       Date:  1994-11       Impact factor: 5.948

10.  Application of PCR to multiple specimen types for diagnosis of cytomegalovirus infection: comparison with cell culture and shell vial assay.

Authors:  M J Miller; S Bovey; K Pado; D A Bruckner; E A Wagar
Journal:  J Clin Microbiol       Date:  1994-01       Impact factor: 5.948
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