Literature DB >> 9003603

A novel Western blot test containing both viral and recombinant proteins for anticytomegalovirus immunoglobulin M detection.

T Lazzarotto1, G T Maine, P Dal Monte, A Ripalti, M P Landini.   

Abstract

We devised a novel Western blot (WB) test for anti-human cytomegalovirus (HCMV) immunoglobulin M (IgM) detection which contains viral structural polypeptides, significant portions of recombinant p150 (ppUL32), and a significant portion of the most immunogenic nonstructural protein p52 (ppUL44). This new test was evaluated in latently infected blood donors, pregnant women, and transplant recipients with ongoing HCMV infection and shown to be more sensitive and specific than traditional WB and conventional enzyme immunoassay for the detection of HCMV-specific IgM.

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Year:  1997        PMID： 9003603      PMCID： PMC229587          DOI： 10.1128/jcm.35.2.393-397.1997

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  28 in total

1.  Enzyme-linked immunoadsorbent assay for the detection of cytomegalovirus-IgM: comparison between eight commercial kits, immunofluorescence, and immunoblotting.

Authors:  T Lazzarotto; B Dalla Casa; B Campisi; M P Landini
Journal:  J Clin Lab Anal       Date:  1992       Impact factor: 2.352

2.  The DNA-binding protein pUL57 of human cytomegalovirus: comparison of specific immunoglobulin M (IgM) reactivity with IgM reactivity to other major target antigens.

Authors:  G T Maine; T Lazzarotto; L E Chovan; R Flanders; M P Landini
Journal:  Clin Diagn Lab Immunol       Date:  1996-05

3.  Detection of serum immunoglobulin M to human cytomegalovirus by western blotting correlates better with virological data than detection by conventional enzyme immunoassay.

Authors:  T Lazzarotto; G T Maine; P Del Monte; H Frush; K Shi; M P Landini
Journal:  Clin Diagn Lab Immunol       Date:  1996-09

4.  Early serodiagnosis of acute human cytomegalovirus infection by enzyme-linked immunosorbent assay using recombinant antigens.

Authors:  R Vornhagen; B Plachter; W Hinderer; T H The; J Van Zanten; L Matter; C A Schmidt; H H Sonneborn; G Jahn
Journal:  J Clin Microbiol       Date:  1994-04       Impact factor: 5.948

5.  Analysis of the humoral response to the flagellin protein of Borrelia burgdorferi: cloning of regions capable of differentiating Lyme disease from syphilis.

Authors:  J M Robinson; T J Pilot-Matias; S D Pratt; C B Patel; T S Bevirt; J C Hunt
Journal:  J Clin Microbiol       Date:  1993-03       Impact factor: 5.948

6.  Recombinant mono- and polyantigens to detect cytomegalovirus-specific immunoglobulin M in human sera by enzyme immunoassay.

Authors:  M P Landini; T Lazzarotto; G T Maine; A Ripalti; R Flanders
Journal:  J Clin Microbiol       Date:  1995-10       Impact factor: 5.948

7.  The DNA-binding protein pUL57 of human cytomegalovirus is a major target antigen for the immunoglobulin M antibody response during acute infection.

Authors:  R Vornhagen; W Hinderer; H H Sonneborn; G Bein; L Matter; T H The; G Jahn; B Plachter
Journal:  J Clin Microbiol       Date:  1995-07       Impact factor: 5.948

8.  Human cytomegalovirus replication correlates with differentiation in a hematopoietic progenitor cell line and can be modulated by HIV-1.

Authors:  T Lazzarotto; G Furlini; M C Re; E Ramazzotti; B Campisi; M P Landini
Journal:  Arch Virol       Date:  1994       Impact factor: 2.574

9.  Construction of a polyepitope fusion antigen of human cytomegalovirus ppUL32 and detection of specific antibodies by ELISA.

Authors:  A Ripalti; M C Boccuni; F Campanini; G Bergamini; T Lazzarotto; M C Battista; B Dalla Casa; M P Landini
Journal:  New Microbiol       Date:  1995-01       Impact factor: 2.479

10.  Direct enzyme-linked immunosorbent assay that uses peroxidase-labeled antigen for determination of immunoglobulin M antibody to cytomegalovirus.

Authors:  A M van Loon; F W Heessen; J T van der Logt; J van der Veen
Journal:  J Clin Microbiol       Date:  1981-03       Impact factor: 5.948
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  11 in total

1.  Avidity of immunoglobulin G directed against human cytomegalovirus during primary and secondary infections in immunocompetent and immunocompromised subjects.

Authors:  T Lazzarotto; P Spezzacatena; P Pradelli; D A Abate; S Varani; M P Landini
Journal:  Clin Diagn Lab Immunol       Date:  1997-07

2.  Search for cytomegalovirus-specific immunoglobulin M: comparison between a new western blot, conventional western blot, and nine commercially available assays.

Authors:  T Lazzarotto; S Brojanac; G T Maine; M P Landini
Journal:  Clin Diagn Lab Immunol       Date:  1997-07

3.  Evaluation of a cytomegalovirus glycoprotein B recombinant enzyme immunoassay to discriminate between a recent and a past infection.

Authors:  M J Sipewa; P Goubau; M Bodéus
Journal:  J Clin Microbiol       Date:  2002-10       Impact factor: 5.948

4.  Development and clinical evaluation of a recombinant-antigen-based cytomegalovirus immunoglobulin M automated immunoassay using the Abbott AxSYM analyzer.

Authors:  G T Maine; R Stricker; M Schuler; J Spesard; S Brojanac; B Iriarte; K Herwig; T Gramins; B Combs; J Wise; H Simmons; T Gram; J Lonze; D Ruzicki; B Byrne; J D Clifton; L E Chovan; D Wachta; C Holas; D Wang; T Wilson; S Tomazic-Allen; M A Clements; G L Wright; T Lazzarotto; A Ripalti; M P Landini
Journal:  J Clin Microbiol       Date:  2000-04       Impact factor: 5.948

5.  Recombinant antibodies encoded by IGHV1-69 react with pUL32, a phosphoprotein of cytomegalovirus and B-cell superantigen.

Authors:  Christoph Steininger; George F Widhopf; Emanuela M Ghia; Christopher S Morello; Katrina Vanura; Rebecca Sanders; Deborah Spector; Don Guiney; Ulrich Jäger; Thomas J Kipps
Journal:  Blood       Date:  2012-01-10       Impact factor: 22.113

Review 6.  Diagnosis and management of human cytomegalovirus infection in the mother, fetus, and newborn infant.

Authors:  Maria Grazia Revello; Giuseppe Gerna
Journal:  Clin Microbiol Rev       Date:  2002-10       Impact factor: 26.132

7.  Development of a new cytomegalovirus (CMV) immunoglobulin M (IgM) immunoblot for detection of CMV-specific IgM.

Authors:  T Lazzarotto; A Ripalti; G Bergamini; M C Battista; P Spezzacatena; F Campanini; P Pradelli; S Varani; L Gabrielli; G T Maine; M P Landini
Journal:  J Clin Microbiol       Date:  1998-11       Impact factor: 5.948

8.  Prenatal diagnosis of congenital cytomegalovirus infection.

Authors:  T Lazzarotto; B Guerra; P Spezzacatena; S Varani; L Gabrielli; P Pradelli; F Rumpianesi; C Banzi; L Bovicelli; M P Landini
Journal:  J Clin Microbiol       Date:  1998-12       Impact factor: 5.948

9.  CMV-associated encephalitis and antineuronal autoantibodies--a case report.

Authors:  Xinling Xu; Peter Bergman; Thomas Willows; Charlotte Tammik; Marie Sund; Tomas Hökfelt; Cecilia Söderberg-Naucler; Stefania Varani
Journal:  BMC Neurol       Date:  2012-09-04       Impact factor: 2.474

10.  Chronic lymphocytic leukemia patients have a preserved cytomegalovirus-specific antibody response despite progressive hypogammaglobulinemia.

Authors:  Katrina Vanura; Franz Rieder; Marie-Theres Kastner; Julia Biebl; Michael Sandhofer; Trang Le; Robert Strassl; Elisabeth Puchhammer-Stöckl; Thomas Perkmann; Christoph F Steininger; Kostas Stamatopoulos; Wolfgang Graninger; Ulrich Jäger; Christoph Steininger
Journal:  PLoS One       Date:  2013-10-23       Impact factor: 3.240
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