Literature DB >> 8705684

The DNA-binding protein pUL57 of human cytomegalovirus: comparison of specific immunoglobulin M (IgM) reactivity with IgM reactivity to other major target antigens.

G T Maine1, T Lazzarotto, L E Chovan, R Flanders, M P Landini.   

Abstract

In this work we used PCR to amplify the DNA regions coding for two polypeptides from pUL57 of human cytomegalovirus (amino acids 540 to 601 and 1144 to 1233) and showed that both portions reacted very efficiently with immunoglobulin M in sera of acutely infected subjects. However, pUL57 is not an essential antigen for the replacement of or supplement to a cocktail of recombinant protein antigens containing portions of ppUL32, -44, -83, and -80a in immunoglobulin M serology.

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Year:  1996        PMID： 8705684      PMCID： PMC170347          DOI： 10.1128/cdli.3.3.358-360.1996

Source DB:  PubMed          Journal:  Clin Diagn Lab Immunol        ISSN： 1071-412X


  15 in total

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3.  Correlation between immunofluorescent detection of human cytomegalovirus immediate early antigens in polymorphonuclear leukocytes and viremia.

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Review 4.  New approaches and perspectives in cytomegalovirus diagnosis.

Authors:  M P Landini
Journal:  Prog Med Virol       Date:  1993

5.  The two major structural phosphoproteins (pp65 and pp150) of human cytomegalovirus and their antigenic properties.

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6.  An Escherichia coli expression vector for high-level production of heterologous proteins in fusion with CMP-KDO synthetase.

Authors:  T J Bolling; W Mandecki
Journal:  Biotechniques       Date:  1990-05       Impact factor: 1.993

7.  Analysis of the humoral response to the flagellin protein of Borrelia burgdorferi: cloning of regions capable of differentiating Lyme disease from syphilis.

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8.  Recombinant mono- and polyantigens to detect cytomegalovirus-specific immunoglobulin M in human sera by enzyme immunoassay.

Authors:  M P Landini; T Lazzarotto; G T Maine; A Ripalti; R Flanders
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9.  Rapid immunodiagnosis of active cytomegalovirus infection by monoclonal antibody staining of blood leucocytes.

Authors:  W van der Bij; R Torensma; W J van Son; J Anema; J Schirm; A M Tegzess; T H The
Journal:  J Med Virol       Date:  1988-06       Impact factor: 2.327

10.  Detection of cytomegalovirus antibodies by an enzyme-linked immunosorbent assay using recombinant polypeptides of the large phosphorylated tegument protein pp150.

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  6 in total

1.  Search for cytomegalovirus-specific immunoglobulin M: comparison between a new western blot, conventional western blot, and nine commercially available assays.

Authors:  T Lazzarotto; S Brojanac; G T Maine; M P Landini
Journal:  Clin Diagn Lab Immunol       Date:  1997-07

2.  A novel Western blot test containing both viral and recombinant proteins for anticytomegalovirus immunoglobulin M detection.

Authors:  T Lazzarotto; G T Maine; P Dal Monte; A Ripalti; M P Landini
Journal:  J Clin Microbiol       Date:  1997-02       Impact factor: 5.948

3.  Development and clinical evaluation of a recombinant-antigen-based cytomegalovirus immunoglobulin M automated immunoassay using the Abbott AxSYM analyzer.

Authors:  G T Maine; R Stricker; M Schuler; J Spesard; S Brojanac; B Iriarte; K Herwig; T Gramins; B Combs; J Wise; H Simmons; T Gram; J Lonze; D Ruzicki; B Byrne; J D Clifton; L E Chovan; D Wachta; C Holas; D Wang; T Wilson; S Tomazic-Allen; M A Clements; G L Wright; T Lazzarotto; A Ripalti; M P Landini
Journal:  J Clin Microbiol       Date:  2000-04       Impact factor: 5.948

4.  Cross-reactivity of Epstein-Barr virus-specific immunoglobulin M antibodies with cytomegalovirus antigens containing glycine homopolymers.

Authors:  D Lang; R Vornhagen; M Rothe; W Hinderer; H H Sonneborn; B Plachter
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5.  Reactivity to p52 and CM2 recombinant proteins in primary human cytomegalovirus infection with a microparticle agglutination assay.

Authors:  E Nulens; M Bodéus; F Bonelli; A Soleti; P Goubau
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6.  Development of a new cytomegalovirus (CMV) immunoglobulin M (IgM) immunoblot for detection of CMV-specific IgM.

Authors:  T Lazzarotto; A Ripalti; G Bergamini; M C Battista; P Spezzacatena; F Campanini; P Pradelli; S Varani; L Gabrielli; G T Maine; M P Landini
Journal:  J Clin Microbiol       Date:  1998-11       Impact factor: 5.948
  6 in total

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