Regeneration of rat calvarial defects using a bioabsorbable membrane technique: influence of collagen cross-linking.

The aim of the present study was to examine the influence of cross-linking on collagen membranes used for guided bone regeneration of calvarial defects in rats. In 48 Wistar rats, divided equally into 4 groups, 1 control and 3 experimental, standardized transosseous circular calvarial defects were made midparietally. In the control group, the defect was only covered by the soft tissue flap while in the 3 experimental groups, 3 differently cross-linked collagen membranes were interposed between the osseous defect and the overlying flap before suturing. The healing was assessed at 10, 20, and 30 days after surgery. The results showed that augmenting the degree of collagen cross-linking diminished the membrane resorption rate. Compared to the sham-operated sites, the membrane protected defects showed significantly more bone regeneration (on average 4 times more) as attested by histology and measured by histomorphometric analysis. Although the bone gain seemed to augment with increasing degrees of cross-linking, the results within the 3 experimental groups were not statistically different. Since longer healing periods might have been necessary to substantiate results within experimental groups, a study is currently undertaken to evaluate this aspect. This study demonstrated the efficacy of collagen membranes in guiding bone regeneration, as well as the importance of the type and degree of cross-linking.