Literature DB >> 8961955

Determination of a transmembrane segment using cysteine-scanning mutants of transposon Tn10-encoded metal-tetracycline/H+ antiporter.

T Kimura1, M Suzuki, T Sawai, A Yamaguchi.   

Abstract

The metal-tetracycline/H+ antiporter is a bacterial plasma membrane protein belonging to the 12-transmembrane transporter family. The boundaries of membrane-embedded domains can be clearly determined by means of a method involving cysteine-scanning mutants and their reactivity to N-ethylmaleimide. The results for 37 cysteine-scanning mutants around putative transmembrane helix 9 indicate that the residues from 266 to 275 and from 296 to 298 form water extruding loops, while residues from 276 to 295 and from 299 to 302 are embedded in the membrane. This method is generally useful for determination of the transmembrane regions of a polytopic membrane protein.

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Year:  1996        PMID： 8961955     DOI： 10.1021/bi961568g

Source DB:  PubMed          Journal:  Biochemistry        ISSN： 0006-2960            Impact factor:   3.162


  6 in total

1.  Energy-driven subunit rotation at the interface between subunit a and the c oligomer in the F(O) sector of Escherichia coli ATP synthase.

Authors:  M L Hutcheon; T M Duncan; H Ngai; R L Cross
Journal:  Proc Natl Acad Sci U S A       Date:  2001-07-03       Impact factor: 11.205

2.  Structural conservation in the major facilitator superfamily as revealed by comparative modeling.

Authors:  Eyal Vardy; Isaiah T Arkin; Kay E Gottschalk; H Ronald Kaback; Shimon Schuldiner
Journal:  Protein Sci       Date:  2004-07       Impact factor: 6.725

3.  Tetracycline antibiotics: mode of action, applications, molecular biology, and epidemiology of bacterial resistance.

Authors:  I Chopra; M Roberts
Journal:  Microbiol Mol Biol Rev       Date:  2001-06       Impact factor: 11.056

4.  Functional importance and local environments of the cysteines in the tetracycline resistance protein encoded by plasmid pBR322.

Authors:  J E Jewell; J Orwick; J Liu; K W Miller
Journal:  J Bacteriol       Date:  1999-03       Impact factor: 3.490

5.  Site-directed mutagenesis studies of selected motif and charged residues and of cysteines of the multifunctional tetracycline efflux protein Tet(L).

Authors:  Jie Jin; Terry A Krulwich
Journal:  J Bacteriol       Date:  2002-03       Impact factor: 3.490

6.  Isolation and characterisation of transport-defective substrate-binding mutants of the tetracycline antiporter TetA(B).

Authors:  David J Wright; Christopher G Tate
Journal:  Biochim Biophys Acta       Date:  2015-07-02
  6 in total

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