Literature DB >> 11872735

Site-directed mutagenesis studies of selected motif and charged residues and of cysteines of the multifunctional tetracycline efflux protein Tet(L).

Jie Jin1, Terry A Krulwich.   

Abstract

All of the transmembrane glutamates of Tet(L) are essential for tetracycline (TET) resistance, and E397 has been shown to be essential for all catalytic modes, i.e., TET-Me(2+) and Na(+) efflux and K(+) uptake. Loop residues D74 and G70 are essential for TET flux but not for Na(+) or K(+) flux. A cysteineless Tet(L) protein exhibits all activities.

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Year:  2002        PMID： 11872735      PMCID： PMC134896          DOI： 10.1128/JB.184.6.1796-1800.2002

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  34 in total

1.  The TetA(K) tetracycline/H(+) antiporter from Staphylococcus aureus: mutagenesis and functional analysis of motif C.

Authors:  S L Ginn; M H Brown; R A Skurray
Journal:  J Bacteriol       Date:  2000-03       Impact factor: 3.490

2.  Membrane topology of the metal-tetracycline/H+ antiporter TetA(K) from Staphylococcus aureus.

Authors:  S L Ginn; M H Brown; R A Skurray
Journal:  J Bacteriol       Date:  1997-06       Impact factor: 3.490

3.  Determination of a transmembrane segment using cysteine-scanning mutants of transposon Tn10-encoded metal-tetracycline/H+ antiporter.

Authors:  T Kimura; M Suzuki; T Sawai; A Yamaguchi
Journal:  Biochemistry       Date:  1996-12-10       Impact factor: 3.162

4.  The purified Bacillus subtilis tetracycline efflux protein TetA(L) reconstitutes both tetracycline-cobalt/H+ and Na+(K+)/H+ exchange.

Authors:  J Cheng; D B Hicks; T A Krulwich
Journal:  Proc Natl Acad Sci U S A       Date:  1996-12-10       Impact factor: 11.205

5.  Cysteine-scanning mutagenesis around transmembrane segment III of Tn10-encoded metal-tetracycline/H+ antiporter.

Authors:  T Kimura; Y Shiina; T Sawai; A Yamaguchi
Journal:  J Biol Chem       Date:  1998-02-27       Impact factor: 5.157

6.  Cysteine-scanning mutagenesis around transmembrane segment VI of Tn10-encoded metal-tetracycline/H(+) antiporter.

Authors:  S Konishi; S Iwaki; T Kimura-Someya; A Yamaguchi
Journal:  FEBS Lett       Date:  1999-11-19       Impact factor: 4.124

7.  Roles of acidic residues in the hydrophilic loop regions of metal-tetracycline/H+ antiporter Tet(K) of Staphylococcus aureus.

Authors:  E Fujihira; T Kimura; A Yamaguchi
Journal:  FEBS Lett       Date:  1997-12-15       Impact factor: 4.124

8.  Membrane topology of the staphylococcal tetracycline efflux protein Tet(K) determined by antibacterial resistance gene fusion.

Authors:  T Hirata; E Fujihira; T Kimura-Someya; A Yamaguchi
Journal:  J Biochem       Date:  1998-12-01       Impact factor: 3.387

9.  Site-directed chemical modification of cysteine-scanning mutants as to transmembrane segment II and its flanking regions of the Tn10-encoded metal-tetracycline/H+ antiporter reveals a transmembrane water-filled channel.

Authors:  T Kimura-Someya; S Iwaki; A Yamaguchi
Journal:  J Biol Chem       Date:  1998-12-04       Impact factor: 5.157

10.  Electrogenic antiport activities of the Gram-positive Tet proteins include a Na+(K+)/K+ mode that mediates net K+ uptake.

Authors:  A A Guffanti; J Cheng; T A Krulwich
Journal:  J Biol Chem       Date:  1998-10-09       Impact factor: 5.157
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  5 in total

1.  Importance of the GP dipeptide of the antiporter motif and other membrane-embedded proline and glycine residues in tetracycline efflux protein Tet(L).

Authors:  Magdia De Jesus; Jie Jin; Arthur A Guffanti; Terry A Krulwich
Journal:  Biochemistry       Date:  2005-09-27       Impact factor: 3.162

2.  Tet(L) and tet(K) tetracycline-divalent metal/H+ antiporters: characterization of multiple catalytic modes and a mutagenesis approach to differences in their efflux substrate and coupling ion preferences.

Authors:  Jie Jin; Arthur A Guffanti; David H Bechhofer; Terry A Krulwich
Journal:  J Bacteriol       Date:  2002-09       Impact factor: 3.490

3.  TetL tetracycline efflux protein from Bacillus subtilis is a dimer in the membrane and in detergent solution.

Authors:  Markus Safferling; Heather Griffith; Jie Jin; Josh Sharp; Magdia De Jesus; Caroline Ng; Terry A Krulwich; Da-Neng Wang
Journal:  Biochemistry       Date:  2003-12-02       Impact factor: 3.162

Review 4.  Multidrug efflux pumps from Enterobacteriaceae, Vibrio cholerae and Staphylococcus aureus bacterial food pathogens.

Authors:  Jody L Andersen; Gui-Xin He; Prathusha Kakarla; Ranjana K C; Sanath Kumar; Wazir Singh Lakra; Mun Mun Mukherjee; Indrika Ranaweera; Ugina Shrestha; Thuy Tran; Manuel F Varela
Journal:  Int J Environ Res Public Health       Date:  2015-01-28       Impact factor: 3.390

Review 5.  Functional and Structural Roles of the Major Facilitator Superfamily Bacterial Multidrug Efflux Pumps.

Authors:  Sanath Kumar; Manjusha Lekshmi; Ammini Parvathi; Manisha Ojha; Nicholas Wenzel; Manuel F Varela
Journal:  Microorganisms       Date:  2020-02-16
  5 in total

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