Literature DB >> 10049405

Functional importance and local environments of the cysteines in the tetracycline resistance protein encoded by plasmid pBR322.

J E Jewell1, J Orwick, J Liu, K W Miller.   

Abstract

The properties of the cysteines in the pBR322-encoded tetracycline resistance protein have been examined. Cysteines are important but not essential for tetracycline transport activity. None of the cysteines reacted with biotin maleimide, suggesting that they are shielded from the aqueous phase or reside in a negatively charged local environment.

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Year:  1999        PMID： 10049405      PMCID： PMC93563     

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  33 in total

1.  Asp-285 of the metal-tetracycline/H+ antiporter of Escherichia coli is essential for substrate binding.

Authors:  T Kimura; A Yamaguchi
Journal:  FEBS Lett       Date:  1996-06-10       Impact factor: 4.124

2.  Efficient insertion of odd-numbered transmembrane segments of the tetracycline resistance protein requires even-numbered segments.

Authors:  D Guo; J Liu; A Motlagh; J Jewell; K W Miller
Journal:  J Biol Chem       Date:  1996-11-29       Impact factor: 5.157

3.  Determination of a transmembrane segment using cysteine-scanning mutants of transposon Tn10-encoded metal-tetracycline/H+ antiporter.

Authors:  T Kimura; M Suzuki; T Sawai; A Yamaguchi
Journal:  Biochemistry       Date:  1996-12-10       Impact factor: 3.162

4.  Identification of a topology control domain in the tetracycline resistance protein.

Authors:  K W Miller; J E Jewell
Journal:  Arch Biochem Biophys       Date:  1995-10-01       Impact factor: 4.013

5.  Sequence of a class E tetracycline resistance gene from Escherichia coli and comparison of related tetracycline efflux proteins.

Authors:  J D Allard; K P Bertrand
Journal:  J Bacteriol       Date:  1993-07       Impact factor: 3.490

6.  Cysteine-scanning mutagenesis around transmembrane segment III of Tn10-encoded metal-tetracycline/H+ antiporter.

Authors:  T Kimura; Y Shiina; T Sawai; A Yamaguchi
Journal:  J Biol Chem       Date:  1998-02-27       Impact factor: 5.157

7.  Enzymatic and biochemical probes of residues external to the translocation pathway of UhpT, the sugar phosphate carrier of Escherichia coli.

Authors:  M Matos; M C Fann; R T Yan; P C Maloney
Journal:  J Biol Chem       Date:  1996-08-02       Impact factor: 5.157

8.  Membrane topology of the transposon 10-encoded metal-tetracycline/H+ antiporter as studied by site-directed chemical labeling.

Authors:  T Kimura; M Ohnuma; T Sawai; A Yamaguchi
Journal:  J Biol Chem       Date:  1997-01-03       Impact factor: 5.157

9.  Membrane topology of a cysteine-less mutant of human P-glycoprotein.

Authors:  T W Loo; D M Clarke
Journal:  J Biol Chem       Date:  1995-01-13       Impact factor: 5.157

10.  Organization in the membrane of the N-terminal proton-translocating domain of the beta subunit of the pyridine nucleotide transhydrogenase of Escherichia coli.

Authors:  N A Glavas; C Hou; P D Bragg
Journal:  Biochem Biophys Res Commun       Date:  1995-09-05       Impact factor: 3.575
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  3 in total

1.  Second-site suppressor mutations of inactivating substitutions at gly247 of the tetracycline efflux protein, Tet(B).

Authors:  C A Saraceni-Richards; S B Levy
Journal:  J Bacteriol       Date:  2000-11       Impact factor: 3.490

2.  Transmembrane protein topology mapping by the substituted cysteine accessibility method (SCAM(TM)): application to lipid-specific membrane protein topogenesis.

Authors:  Mikhail Bogdanov; Wei Zhang; Jun Xie; William Dowhan
Journal:  Methods       Date:  2005-06       Impact factor: 3.608

3.  Copper/zinc-Superoxide dismutase is required for oxytetracycline resistance of Saccharomyces cerevisiae.

Authors:  S V Avery; S Malkapuram; C Mateus; K S Babb
Journal:  J Bacteriol       Date:  2000-01       Impact factor: 3.490
  3 in total

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