Literature DB >> 8953736

Ethanolic fermentation of xylose with Saccharomyces cerevisiae harboring the Thermus thermophilus xylA gene, which expresses an active xylose (glucose) isomerase.

M Walfridsson1, X Bao, M Anderlund, G Lilius, L Bülow, B Hahn-Hägerdal.   

Abstract

The Thermus thermophilus xylA gene encoding xylose (glucose) isomerase was cloned and expressed in Saccharomyces cerevisiae under the control of the yeast PGK1 promoter. The recombinant xylose isomerase showed the highest activity at 85 degrees C with a specific activity of 1.0 U mg-1. A new functional metabolic pathway in S. cerevisiae with ethanol formation during oxygen-limited xylose fermentation was demonstrated. Xylitol and acetic acid were also formed during the fermentation.

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Year:  1996        PMID： 8953736      PMCID： PMC168291          DOI： 10.1128/aem.62.12.4648-4651.1996

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  10 in total

1.  Expression of the Escherichia coli xylose isomerase gene in Saccharomyces cerevisiae.

Authors:  A V Sarthy; B L McConaughy; Z Lobo; J A Sundstrom; C E Furlong; B D Hall
Journal:  Appl Environ Microbiol       Date:  1987-09       Impact factor: 4.792

2.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

Review 4.  Utilization of xylose by bacteria, yeasts, and fungi.

Authors:  T W Jeffries
Journal:  Adv Biochem Eng Biotechnol       Date:  1983       Impact factor: 2.635

5.  Xylose (glucose) isomerase gene from the thermophile Thermus thermophilus: cloning, sequencing, and comparison with other thermostable xylose isomerases.

Authors:  K Dekker; H Yamagata; K Sakaguchi; S Udaka
Journal:  J Bacteriol       Date:  1991-05       Impact factor: 3.490

6.  High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier.

Authors:  R H Schiestl; R D Gietz
Journal:  Curr Genet       Date:  1989-12       Impact factor: 3.886

7.  Xylose-metabolizing Saccharomyces cerevisiae strains overexpressing the TKL1 and TAL1 genes encoding the pentose phosphate pathway enzymes transketolase and transaldolase.

Authors:  M Walfridsson; J Hallborn; M Penttilä; S Keränen; B Hahn-Hägerdal
Journal:  Appl Environ Microbiol       Date:  1995-12       Impact factor: 4.792

8.  Efficient synthesis of enzymatically active calf chymosin in Saccharomyces cerevisiae.

Authors:  J Mellor; M J Dobson; N A Roberts; M F Tuite; J S Emtage; S White; P A Lowe; T Patel; A J Kingsman; S M Kingsman
Journal:  Gene       Date:  1983-09       Impact factor: 3.688

9.  Xylulose and glucose fermentation by Saccharomyces cerevisiae in chemostat culture.

Authors:  H Jeppsson; S Yu; B Hahn-Hägerdal
Journal:  Appl Environ Microbiol       Date:  1996-05       Impact factor: 4.792

10.  Purification and partial characterization of an aldo-keto reductase from Saccharomyces cerevisiae.

Authors:  A Kuhn; C van Zyl; A van Tonder; B A Prior
Journal:  Appl Environ Microbiol       Date:  1995-04       Impact factor: 4.792
  10 in total
  60 in total

1.  Microbiome of fungus-growing termites: a new reservoir for lignocellulase genes.

Authors:  Ning Liu; Xing Yan; Meiling Zhang; Lei Xie; Qian Wang; Yongping Huang; Xuguo Zhou; Shengyue Wang; Zhihua Zhou
Journal:  Appl Environ Microbiol       Date:  2010-11-05       Impact factor: 4.792

Review 2.  Metabolic engineering of Saccharomyces cerevisiae.

Authors:  S Ostergaard; L Olsson; J Nielsen
Journal:  Microbiol Mol Biol Rev       Date:  2000-03       Impact factor: 11.056

3.  Improved xylose fermentation of Kluyveromyces marxianus at elevated temperature through construction of a xylose isomerase pathway.

Authors:  Rongliang Wang; Lulu Li; Biao Zhang; Xiaolian Gao; Dongmei Wang; Jiong Hong
Journal:  J Ind Microbiol Biotechnol       Date:  2013-05-09       Impact factor: 3.346

4.  Co-utilization of glucose and xylose by evolved Thermus thermophilus LC113 strain elucidated by (13)C metabolic flux analysis and whole genome sequencing.

Authors:  Lauren T Cordova; Jing Lu; Robert M Cipolla; Nicholas R Sandoval; Christopher P Long; Maciek R Antoniewicz
Journal:  Metab Eng       Date:  2016-05-07       Impact factor: 9.783

5.  A modified Saccharomyces cerevisiae strain that consumes L-Arabinose and produces ethanol.

Authors:  Jessica Becker; Eckhard Boles
Journal:  Appl Environ Microbiol       Date:  2003-07       Impact factor: 4.792

6.  Functional expression of a bacterial xylose isomerase in Saccharomyces cerevisiae.

Authors:  Dawid Brat; Eckhard Boles; Beate Wiedemann
Journal:  Appl Environ Microbiol       Date:  2009-02-13       Impact factor: 4.792

7.  Bulk segregant analysis by high-throughput sequencing reveals a novel xylose utilization gene from Saccharomyces cerevisiae.

Authors:  Jared W Wenger; Katja Schwartz; Gavin Sherlock
Journal:  PLoS Genet       Date:  2010-05-13       Impact factor: 5.917

8.  Optimizing pentose utilization in yeast: the need for novel tools and approaches.

Authors:  Eric Young; Sun-Mi Lee; Hal Alper
Journal:  Biotechnol Biofuels       Date:  2010-11-16       Impact factor: 6.040

Review 9.  The path to next generation biofuels: successes and challenges in the era of synthetic biology.

Authors:  Clementina Dellomonaco; Fabio Fava; Ramon Gonzalez
Journal:  Microb Cell Fact       Date:  2010-01-20       Impact factor: 5.328

10.  Increased expression of the oxidative pentose phosphate pathway and gluconeogenesis in anaerobically growing xylose-utilizing Saccharomyces cerevisiae.

Authors:  David Runquist; Bärbel Hahn-Hägerdal; Maurizio Bettiga
Journal:  Microb Cell Fact       Date:  2009-09-24       Impact factor: 5.328
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