Literature DB >> 8918820

Improved immobilization of DNA to microwell plates for DNA-DNA hybridization.

H Hirayama1, J Tamaoka, K Horikoshi.   

Abstract

An improved and simplified protocol for DNA immobilization was developed to enhance DNA-DNA hybridization on microwell plates. Target DNA was immobilized by simple dry-adsorption. Efficiencies of DNA immobilization and retention were enhanced 1.4-6.5 times and 4.2-19.6 times, respectively, compared with a conventional method. The overall hybridization efficiency was increased 3.1-5.2 times. This simple new protocol can reduce the consumption of scarce DNA samples.

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Year:  1996        PMID： 8918820      PMCID： PMC146194          DOI： 10.1093/nar/24.20.4098

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  7 in total

1.  Covalent immobilization of DNA onto polystyrene microwells: the molecules are only bound at the 5' end.

Authors:  S R Rasmussen; M R Larsen; S E Rasmussen
Journal:  Anal Biochem       Date:  1991-10       Impact factor: 3.365

2.  Microplate hybridization of amplified viral DNA segment.

Authors:  S Inouye; R Hondo
Journal:  J Clin Microbiol       Date:  1990-06       Impact factor: 5.948

3.  Non-radioactive hybridization probes prepared by the chemical labelling of DNA and RNA with a novel reagent, photobiotin.

Authors:  A C Forster; J L McInnes; D C Skingle; R H Symons
Journal:  Nucleic Acids Res       Date:  1985-02-11       Impact factor: 16.971

4.  Improved microplate immunoenzymatic assay of PCR products for rapid detection of Mycoplasma pneumoniae.

Authors:  A Vekris; F Bauduer; S Maillet; C Bébéar; J Bonnet
Journal:  Mol Cell Probes       Date:  1995-02       Impact factor: 2.365

5.  Small-scale DNA preparation for rapid genetic identification of Campylobacter species without radioisotope.

Authors:  T Ezaki; N Takeuchi; S L Liu; A Kai; H Yamamoto; E Yabuuchi
Journal:  Microbiol Immunol       Date:  1988       Impact factor: 1.955

6.  Quantification of picogram levels of specific DNA immobilized in microtiter wells.

Authors:  Y Nagata; H Yokota; O Kosuda; K Yokoo; K Takemura; T Kikuchi
Journal:  FEBS Lett       Date:  1985-04-22       Impact factor: 4.124

7.  Detection of Mycoplasma pneumoniae by polymerase chain reaction and nonradioactive hybridization in microtiter plates.

Authors:  E Lüneberg; J S Jensen; M Frosch
Journal:  J Clin Microbiol       Date:  1993-05       Impact factor: 5.948
  7 in total
  3 in total

1.  Heat-induced formation of reactive oxygen species and 8-oxoguanine, a biomarker of damage to DNA.

Authors:  Vadim I Bruskov; Lyudmila V Malakhova; Zhaksylyk K Masalimov; Anatoly V Chernikov
Journal:  Nucleic Acids Res       Date:  2002-03-15       Impact factor: 16.971

2.  Biolayer interferometry provides a robust method for detecting DNA binding small molecules in microbial extracts.

Authors:  Ross D Overacker; Birte Plitzko; Sandra Loesgen
Journal:  Anal Bioanal Chem       Date:  2020-11-25       Impact factor: 4.142

3.  Electrochemical DNA hybridization sensors based on conducting polymers.

Authors:  Md Mahbubur Rahman; Xiao-Bo Li; Nasrin Siraj Lopa; Sang Jung Ahn; Jae-Joon Lee
Journal:  Sensors (Basel)       Date:  2015-02-05       Impact factor: 3.576
  3 in total

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