Literature DB >> 3157598

Quantification of picogram levels of specific DNA immobilized in microtiter wells.

Y Nagata, H Yokota, O Kosuda, K Yokoo, K Takemura, T Kikuchi.   

Abstract

A non-radioisotopic and sensitive method for quantification of specific DNA immobilized in microtiter wells has been developed. This method is based upon the immobilization of DNA in microtiter wells and hybridization with biotinylated DNA probe which is followed by complexing with avidin-beta-galactosidase. By measuring fluorescence emitted from the hydrolyzed product by beta-galactosidase of 4-methylumbelliferyl-beta-D-galactoside, it has become possible to quantify a few picograms of specific DNA in DNA samples immobilized in plastic microtiter wells.

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Year:  1985        PMID： 3157598     DOI： 10.1016/0014-5793(85)80814-0

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  17 in total

1.  Development of a rapid assay for determining the relative abundance of bacteria.

Authors:  Arlene K Rowan; Russell J Davenport; Jason R Snape; David Fearnside; Michael R Barer; Thomas P Curtis; Ian M Head
Journal:  Appl Environ Microbiol       Date:  2005-12       Impact factor: 4.792

2.  Preparation of europium-labelled DNA probes and their properties.

Authors:  P Hurskainen; P Dahlén; J Ylikoski; M Kwiatkowski; H Siitari; T Lövgren
Journal:  Nucleic Acids Res       Date:  1991-03-11       Impact factor: 16.971

3.  Microplate hybridization of amplified viral DNA segment.

Authors:  S Inouye; R Hondo
Journal:  J Clin Microbiol       Date:  1990-06       Impact factor: 5.948

4.  Improved immobilization of DNA to microwell plates for DNA-DNA hybridization.

Authors:  H Hirayama; J Tamaoka; K Horikoshi
Journal:  Nucleic Acids Res       Date:  1996-10-15       Impact factor: 16.971

5.  Rapid, fluorometric DNA determination for chick limb-bud mesenchymal-cell microcultures.

Authors:  D F Paulsen; W D Chen; T Scineaux; D Adams
Journal:  In Vitro Cell Dev Biol Anim       Date:  1998-02       Impact factor: 2.416

6.  Detection of hepatitis B virus DNA in serum by polymerase chain reaction amplification and microtiter sandwich hybridization.

Authors:  G H Keller; D P Huang; J W Shih; M M Manak
Journal:  J Clin Microbiol       Date:  1990-06       Impact factor: 5.948

7.  A new biotinylating system for DNA using biotin aminocaproyl hydrazide and glutaraldehyde.

Authors:  T Takahashi; H Arakawa; M Maeda; A Tsuji
Journal:  Nucleic Acids Res       Date:  1989-06-26       Impact factor: 16.971

8.  Simple genetic method to identify viridans group streptococci by colorimetric dot hybridization and fluorometric hybridization in microdilution wells.

Authors:  T Ezaki; Y Hashimoto; N Takeuchi; H Yamamoto; S L Liu; H Miura; K Matsui; E Yabuuchi
Journal:  J Clin Microbiol       Date:  1988-09       Impact factor: 5.948

9.  Improved detection by time-resolved fluorometry of specific DNA immobilized in microtiter wells with europium/metal-chelator labelled DNA probes.

Authors:  A Oser; G Valet
Journal:  Nucleic Acids Res       Date:  1988-08-25       Impact factor: 16.971

10.  Synthesis and hybridization of a series of biotinylated oligonucleotides.

Authors:  A F Cook; E Vuocolo; C L Brakel
Journal:  Nucleic Acids Res       Date:  1988-05-11       Impact factor: 16.971
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