Literature DB >> 1789415

Covalent immobilization of DNA onto polystyrene microwells: the molecules are only bound at the 5' end.

S R Rasmussen1, M R Larsen, S E Rasmussen.   

Abstract

Carbodiimide-mediated condensation of DNA onto microwells is investigated. DNA is bound onto the microwells by formation of a phosphoramidate bond between the 5' terminal phosphate group and the microwells. Immobilization of 25 to 30 ng DNA per well is obtained. DNA molecules bound covalently at only the 5' end are, ideally, perfect for hybridization. The practicability of DNA molecules bound to microwells for hybridization is investigated.

Entities:  

Mesh：

Substances：

Year:  1991        PMID： 1789415     DOI： 10.1016/0003-2697(91)90518-x

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  23 in total

1.  Use of immobilized PCR primers to generate covalently immobilized DNAs for in vitro transcription/translation reactions.

Authors:  J D Andreadis; L A Chrisey
Journal:  Nucleic Acids Res       Date:  2000-01-15       Impact factor: 16.971

2.  Solid phase DNA amplification: characterisation of primer attachment and amplification mechanisms.

Authors:  C Adessi; G Matton; G Ayala; G Turcatti; J J Mermod; P Mayer; E Kawashima
Journal:  Nucleic Acids Res       Date:  2000-10-15       Impact factor: 16.971

3.  A competitive enzyme hybridization assay for plasma determination of phosphodiester and phosphorothioate antisense oligonucleotides.

Authors:  J R Deverre; V Boutet; D Boquet; E Ezan; J Grassi; J M Grognet
Journal:  Nucleic Acids Res       Date:  1997-09-15       Impact factor: 16.971

4.  Development of chemically modified glass surfaces for nucleic acid, protein and small molecule microarrays.

Authors:  László Hackler; György Dormán; Zoltán Kele; László Urge; Ferenc Darvas; László G Puskás
Journal:  Mol Divers       Date:  2003       Impact factor: 2.943

5.  Covalent attachment of DNA to glass supports using a new silane coupling agent and chemiluminescent detection.

Authors:  G Zhang; Y Zhou; X Wu; J Yuan; S Ren
Journal:  J Tongji Med Univ       Date:  2000

6.  Rapid preparation of RNA samples for NMR spectroscopy and X-ray crystallography.

Authors:  Hae-Kap Cheong; Eunha Hwang; Chulhyun Lee; Byong-Seok Choi; Chaejoon Cheong
Journal:  Nucleic Acids Res       Date:  2004-06-15       Impact factor: 16.971

Review 7.  Diamond and biology.

Authors:  Christoph E Nebel; Dongchan Shin; Bohuslav Rezek; Norio Tokuda; Hiroshi Uetsuka; Hideyuki Watanabe
Journal:  J R Soc Interface       Date:  2007-06-22       Impact factor: 4.118

8.  Determination of domoic acid by two different versions of a competitive enzyme-linked immunosorbent assay (ELISA).

Authors:  M Osada; L J Marks; J E Stewart
Journal:  Bull Environ Contam Toxicol       Date:  1995-06       Impact factor: 2.151

9.  Improved immobilization of DNA to microwell plates for DNA-DNA hybridization.

Authors:  H Hirayama; J Tamaoka; K Horikoshi
Journal:  Nucleic Acids Res       Date:  1996-10-15       Impact factor: 16.971

10.  Development of a sensitive multi-well colorimetric assay for active NFkappaB.

Authors:  P Renard; I Ernest; A Houbion; M Art; H Le Calvez; M Raes; J Remacle
Journal:  Nucleic Acids Res       Date:  2001-02-15       Impact factor: 16.971
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.