Literature DB >> 8910037

Immunohistochemical detection of retinoic acid receptor alpha in human skin: a comparison of different fixation protocols.

S Epple1, M Mittmann, J Reichrath.   

Abstract

A technique for the immunohistochemical detection of retinoic acid receptor alpha in cryostat sections of normal human skin in situ has been developed. A highly specific mouse monoclonal antibody, directed against the F region of retinoic acid receptor alpha, was used and a panel of 10 fixation protocols investigated. A three-step protocol, consisting of sequential fixation in 3.7% paraformaldehyde, methanol and acetone, revealed strong nuclear immunoreactivity in epidermal keratinocytes and other cell types present in normal human skin. Other fixation protocols, including fixation regimens using formaldehyde or Carnoy's solution, were less suitable or unsuitable for detection of the receptor in cryostat sections of human skin.

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Year:  1996        PMID： 8910037     DOI： 10.1007/bf02331387

Source DB:  PubMed          Journal:  Histochem J        ISSN： 0018-2214


  8 in total

1.  Antibodies specific to the retinoic acid human nuclear receptors alpha and beta.

Authors:  M P Gaub; Y Lutz; E Ruberte; M Petkovich; N Brand; P Chambon
Journal:  Proc Natl Acad Sci U S A       Date:  1989-05       Impact factor: 11.205

2.  The unlabeled antibody enzyme method of immunohistochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes.

Authors:  L A Sternberger; P H Hardy; J J Cuculis; H G Meyer
Journal:  J Histochem Cytochem       Date:  1970-05       Impact factor: 2.479

3.  Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67.

Authors:  J Gerdes; H Lemke; H Baisch; H H Wacker; U Schwab; H Stein
Journal:  J Immunol       Date:  1984-10       Impact factor: 5.422

4.  In situ detection of retinoid-X receptor expression in normal and psoriatic human skin.

Authors:  J Reichrath; T Münssinger; A Kerber; C Rochette-Egly; P Chambon; F A Bahmer; H P Baum
Journal:  Br J Dermatol       Date:  1995-08       Impact factor: 9.302

5.  Fixation requirements for the immunohistochemical reactivity of PCNA antibody PC10 on cryostat sections.

Authors:  H P Baum; J Reichrath; A Theobald; G Schock
Journal:  Histochem J       Date:  1994-12

6.  Immunological identification and functional quantitation of retinoic acid and retinoid X receptor proteins in human skin.

Authors:  G J Fisher; H S Talwar; J H Xiao; S C Datta; A P Reddy; M P Gaub; C Rochette-Egly; P Chambon; J J Voorhees
Journal:  J Biol Chem       Date:  1994-08-12       Impact factor: 5.157

7.  Immunodetection of multiple species of retinoic acid receptor alpha: evidence for phosphorylation.

Authors:  M P Gaub; C Rochette-Egly; Y Lutz; S Ali; H Matthes; I Scheuer; P Chambon
Journal:  Exp Cell Res       Date:  1992-08       Impact factor: 3.905

Review 8.  The retinoid signaling pathway: molecular and genetic analyses.

Authors:  P Chambon
Journal:  Semin Cell Biol       Date:  1994-04
  8 in total

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