Literature DB >> 7896568

Fixation requirements for the immunohistochemical reactivity of PCNA antibody PC10 on cryostat sections.

H P Baum1, J Reichrath, A Theobald, G Schock.   

Abstract

In contrast to that in paraffin-embedded tissue, the reactivity of monoclonal PCNA antibody PC10 on cryostat sections requires a special fixation procedure as the target epitope is seemingly not accessible to its antibody. A panel of 18 fixation protocols was investigated. Chilled methanol or acetone, or PLP (paraformaldehyde-lysine-periodate) was found to be unsuitable for skin preparations. A two-step fixation protocol was developed for normal skin and basal cell carcinomas. They were fixed first in 3.4% buffered formaldehyde, followed by fixation in 2:1 v/v ethanol-acetic acid. Following this fixation regime, cryostat sections displayed the same PCNA/PC10 labelling pattern as paraffin sections of formalin-fixed tissue.

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Year:  1994        PMID： 7896568

Source DB:  PubMed          Journal:  Histochem J        ISSN： 0018-2214


  18 in total

1.  The complexities of proliferating cell nuclear antigen.

Authors:  D McCormick; P A Hall
Journal:  Histopathology       Date:  1992-12       Impact factor: 5.087

2.  Cyclin/PCNA immunostaining as an alternative to tritiated thymidine pulse labelling for marking S phase cells in paraffin sections from animal and human tissues.

Authors:  P Galand; C Degraef
Journal:  Cell Tissue Kinet       Date:  1989-09

3.  Expression of proliferating cell nuclear antigen/cyclin in human keratinocytes.

Authors:  S Miyagawa; N Okada; Y Takasaki; T Iida; Y Kitano; K Yoshikawa; K Sakamoto; M L Steinberg
Journal:  J Invest Dermatol       Date:  1989-11       Impact factor: 8.551

4.  Acetone/periodate-lysine-paraformaldehyde (PLP) fixation and improved morphology of cryostat sections for immunohistochemistry.

Authors:  P A Hall; P M Stearn; M G Butler; A J D'Ardenne
Journal:  Histopathology       Date:  1987-01       Impact factor: 5.087

5.  Purification and N-terminal amino acid sequence of proliferating cell nuclear antigen (PCNA)/cyclin and development of ELISA for anti-PCNA antibodies.

Authors:  K Ogata; Y Ogata; R M Nakamura; E M Tan
Journal:  J Immunol       Date:  1985-10       Impact factor: 5.422

6.  Analysis of the epitopes of proliferating cell nuclear antigen recognized by monoclonal antibodies.

Authors:  G Roos; G Landberg; J P Huff; R Houghten; Y Takasaki; E M Tan
Journal:  Lab Invest       Date:  1993-02       Impact factor: 5.662

7.  Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms.

Authors:  P A Hall; D A Levison; A L Woods; C C Yu; D B Kellock; J A Watkins; D M Barnes; C E Gillett; R Camplejohn; R Dover
Journal:  J Pathol       Date:  1990-12       Impact factor: 7.996

8.  Measurement of S phase duration in human epidermis using cyclin immunostaining and 3H-thymidine pulse labelling.

Authors:  E Berlingin; M Heenen; P Galand
Journal:  Arch Dermatol Res       Date:  1992       Impact factor: 3.017

9.  Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form.

Authors:  N H Waseem; D P Lane
Journal:  J Cell Sci       Date:  1990-05       Impact factor: 5.285

10.  Existence of two populations of cyclin/proliferating cell nuclear antigen during the cell cycle: association with DNA replication sites.

Authors:  R Bravo; H Macdonald-Bravo
Journal:  J Cell Biol       Date:  1987-10       Impact factor: 10.539
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  1 in total

1.  Immunohistochemical detection of retinoic acid receptor alpha in human skin: a comparison of different fixation protocols.

Authors:  S Epple; M Mittmann; J Reichrath
Journal:  Histochem J       Date:  1996-09
  1 in total

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