Literature DB >> 8887365

Quantitation and purification of polymerase chain reaction products by high-performance liquid chromatography.

E D Katz1.   

Abstract

This work describes the application of the fully automated high-performance liquid chromatographic system to the analysis of PCR-amplified products. Efficient separations of both DNA restriction fragments and PCR products were performed using an anion-exchange DEAE-NPR column, packed with 2.5-microns nonporous particles. The automated HPLC method was employed for the separation, quantitation, and purification of PCR products in less than 10 min in a single step.

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Year:  1996        PMID: 8887365     DOI: 10.1007/BF02762327

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN: 1073-6085            Impact factor:   2.695


  11 in total

1.  Rapid separation, quantitation and purification of products of polymerase chain reaction by liquid chromatography.

Authors:  E D Katz; L A Haff; R Eksteen
Journal:  J Chromatogr       Date:  1990-07-20

Review 2.  Recent advances in the polymerase chain reaction.

Authors:  H A Erlich; D Gelfand; J J Sninsky
Journal:  Science       Date:  1991-06-21       Impact factor: 47.728

3.  T-cell receptor variable beta genes show differential expression in CD4 and CD8 T cells.

Authors:  M P Davey; M M Meyer; D D Munkirs; D Babcock; M P Braun; J B Hayden; A C Bakke
Journal:  Hum Immunol       Date:  1991-11       Impact factor: 2.850

4.  Rapid analysis and quantitation of PCR products by high-performance liquid chromatography.

Authors:  W Warren; T Wheat; P Knudsen
Journal:  Biotechniques       Date:  1991-08       Impact factor: 1.993

5.  Nonradioactive quantification of mdr1 mRNA by polymerase chain reaction amplification coupled with HPLC.

Authors:  B van Hille; A Lohri; J Reuter; R Herrmann
Journal:  Clin Chem       Date:  1995-08       Impact factor: 8.327

6.  Separation of DNA restriction fragments by high-performance ion-exchange chromatography on a non-porous ion exchanger.

Authors:  Y Kato; Y Yamasaki; A Onaka; T Kitamura; T Hashimoto; T Murotsu; S Fukushige; K Matsubara
Journal:  J Chromatogr       Date:  1989-09-08

7.  Rapid analysis and purification of polymerase chain reaction products by high-performance liquid chromatography.

Authors:  E D Katz; M W Dong
Journal:  Biotechniques       Date:  1990-05       Impact factor: 1.993

8.  Evolution of polymerase chain reaction to a quantitative laboratory tool.

Authors:  R A McPherson
Journal:  Clin Chem       Date:  1995-08       Impact factor: 8.327

9.  High-performance liquid chromatography to assess the effect of serum storage conditions on the detection of hepatitis C virus by the polymerase chain reaction.

Authors:  C M Quan; M Krajden; J Zhao; A W Chan
Journal:  J Virol Methods       Date:  1993-08       Impact factor: 2.014

10.  Curved DNA fragments display retarded elution upon anion exchange HPLC.

Authors:  F Fack; V Sarantoglou
Journal:  Nucleic Acids Res       Date:  1991-08-11       Impact factor: 16.971

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