Literature DB >> 2357377

Rapid analysis and purification of polymerase chain reaction products by high-performance liquid chromatography.

E D Katz1, M W Dong.   

Abstract

This report describes the use of high-performance liquid chromatography (HPLC) for the rapid analysis and purification of the polymerase chain reaction products. Employing a new anion-exchange nonporous column, efficient separations of both DNA restriction fragments and amplified PCR products are achieved in 10 to 20 minutes and quantitated within +/- 10%. The performance of the HPLC technique is described in terms of resolution, reproductibility, sensitivity and micropreparative capability and compared to that of gel electrophoresis for this application.

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Year:  1990        PMID： 2357377

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  12 in total

1.  Purification and characterization of PCR-inhibitory components in blood cells.

Authors:  W A Al-Soud; P Rådström
Journal:  J Clin Microbiol       Date:  2001-02       Impact factor: 5.948

2.  Genotypic identification and characterization of species and strains within the genus Candida by using random amplified polymorphic DNA.

Authors:  P F Lehmann; D Lin; B A Lasker
Journal:  J Clin Microbiol       Date:  1992-12       Impact factor: 5.948

3.  Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications.

Authors:  Q Chou; M Russell; D E Birch; J Raymond; W Bloch
Journal:  Nucleic Acids Res       Date:  1992-04-11       Impact factor: 16.971

4.  Quantitation and purification of polymerase chain reaction products by high-performance liquid chromatography.

Authors:  E D Katz
Journal:  Mol Biotechnol       Date:  1996-08       Impact factor: 2.695

5.  Expression of de novo high-lysine alpha-helical coiled-coil proteins may significantly increase the accumulated levels of lysine in mature seeds of transgenic tobacco plants.

Authors:  S J Keeler; C L Maloney; P Y Webber; C Patterson; L T Hirata; S C Falco; J A Rice
Journal:  Plant Mol Biol       Date:  1997-05       Impact factor: 4.076

6.  Quantitative analysis of human multidrug resistance 1 (MDR1) gene expression by nonisotopic competitive reverse transcriptase polymerase chain reaction assay.

Authors:  H Kobayashi; Y Takemura; H Miyachi; M Kawabata; S Mori; Y Kawai; K Furihata; S Sekiguchi; K Watanabe
Journal:  J Clin Lab Anal       Date:  1997       Impact factor: 2.352

7.  Quantitative reverse transcription-PCR analysis of Legionella pneumophila-induced cytokine mRNA in different macrophage populations by high-performance liquid chromatography.

Authors:  Y Yamamoto; C Retzlaff; P He; T W Klein; H Friedman
Journal:  Clin Diagn Lab Immunol       Date:  1995-01

8.  Efficient large-scale purification of restriction fragments by solute-displacement ion-exchange HPLC.

Authors:  J H Waterborg; A J Robertson
Journal:  Nucleic Acids Res       Date:  1993-06-25       Impact factor: 16.971

9.  Bacterial heat shock proteins directly induce cytokine mRNA and interleukin-1 secretion in macrophage cultures.

Authors:  C Retzlaff; Y Yamamoto; P S Hoffman; H Friedman; T W Klein
Journal:  Infect Immun       Date:  1994-12       Impact factor: 3.441

10.  Hormonal regulation of the zonated expression of cytochrome P-450 3A in rat liver.

Authors:  T Oinonen; K O Lindros
Journal:  Biochem J       Date:  1995-07-01       Impact factor: 3.857
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