Literature DB >> 8877146

Monoclonal antibodies to the enterotoxin of Bacteroides fragilis: production, characterization, and immunodiagnostic application.

F Qadri1, M G Mohi, A Chowdhury, K Alam, T Azim, C Sears, R B Sack, M J Albert.   

Abstract

A monoclonal antibody, ICT11, specific for the toxin of enterotoxigenic Bacteroides fragilis (ETBF) neutralized the cytotoxic effect of the toxin on human colonic cell line HT-29/C1. In an evaluation using 115 diarrheal stool specimens and culture as the "gold standard," the assay showed a sensitivity of 85% and a specificity of 100%. An ICT11-based sandwich enzyme-linked immunosorbent assay showed a sensitivity of 100% and a specificity of 98% for direct detection of toxin from stool samples compared with those of culture. Thus, ICT11-based assays will be useful for screening for ETBF.

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Year:  1996        PMID： 8877146      PMCID： PMC170417          DOI： 10.1128/cdli.3.5.608-610.1996

Source DB:  PubMed          Journal:  Clin Diagn Lab Immunol        ISSN： 1071-412X


  15 in total

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Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

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Authors:  F Qadri; T Azim; A Hossain; D Islam; G Mondol; S M Faruque; M J Albert
Journal:  FEMS Microbiol Lett       Date:  1993-03-01       Impact factor: 2.742

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Authors:  L L Myers; D S Shoop; J E Collins
Journal:  J Clin Microbiol       Date:  1990-07       Impact factor: 5.948

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Authors:  L L Myers; B D Firehammer; D S Shoop; M M Border
Journal:  Infect Immun       Date:  1984-05       Impact factor: 3.441

5.  Enterovirulence of enterotoxigenic Bacteroides fragilis in gnotobiotic pigs.

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Journal:  Vet Pathol       Date:  1991-11       Impact factor: 2.221

6.  Isolation of enterotoxigenic Bacteroides fragilis from Bangladeshi children with diarrhea: a controlled study.

Authors:  R B Sack; M J Albert; K Alam; P K Neogi; M S Akbar
Journal:  J Clin Microbiol       Date:  1994-04       Impact factor: 5.948

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8.  Human colonic epithelial cells, HT29/C1, treated with crude Bacteroides fragilis enterotoxin dramatically alter their morphology.

Authors:  C S Weikel; F D Grieco; J Reuben; L L Myers; R B Sack
Journal:  Infect Immun       Date:  1992-02       Impact factor: 3.441

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Authors:  V H San Joaquin; J C Griffis; C Lee; C L Sears
Journal:  Scand J Infect Dis       Date:  1995

10.  Pathogenesis of Shigella diarrhea. IX. Simplified high yield purification of Shigella toxin and characterization of subunit composition and function by the use of subunit-specific monoclonal and polyclonal antibodies.

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Journal:  J Exp Med       Date:  1984-12-01       Impact factor: 14.307
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  2 in total

1.  Production of a mouse antiserum to Bacteroides fragilis enterotoxin using a recombinant enterotoxin precursor.

Authors:  S Sandini; A Scotto d'Abusco; R La Valle; A Pantosti
Journal:  Clin Diagn Lab Immunol       Date:  2001-01

Review 2.  Enterotoxigenic Bacteroides fragilis: a rogue among symbiotes.

Authors:  Cynthia L Sears
Journal:  Clin Microbiol Rev       Date:  2009-04       Impact factor: 26.132
  2 in total

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