Literature DB >> 11139218

Production of a mouse antiserum to Bacteroides fragilis enterotoxin using a recombinant enterotoxin precursor.

S Sandini1, A Scotto d'Abusco, R La Valle, A Pantosti.   

Abstract

The precursor of the Bacteroides fragilis metalloprotease enterotoxin was cloned and expressed in Escherichia coli, which was not able to process the precursor into the biologically active enterotoxin. Mouse antiserum elicited to the recombinant precursor reacted with the purified enterotoxin and with a crude enterotoxin preparation from an enterotoxigenic strain. The antiserum neutralized the cytotoxic activity of the enterotoxin in HT-29 cells.

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Year:  2001        PMID： 11139218      PMCID： PMC96033          DOI： 10.1128/CDLI.8.1.190-191.2001

Source DB:  PubMed          Journal:  Clin Diagn Lab Immunol        ISSN： 1071-412X


  15 in total

1.  The alleles of the bft gene are distributed differently among enterotoxigenic Bacteroides fragilis strains from human sources and can be present in double copies.

Authors:  A S Scotto d'Abusco; M Del Grosso; S Censini; A Covacci; A Pantosti
Journal:  J Clin Microbiol       Date:  2000-02       Impact factor: 5.948

2.  Cloning and characterization of the Bacteroides fragilis metalloprotease toxin gene.

Authors:  A A Franco; L M Mundy; M Trucksis; S Wu; J B Kaper; C L Sears
Journal:  Infect Immun       Date:  1997-03       Impact factor: 3.441

3.  Monoclonal antibodies to the enterotoxin of Bacteroides fragilis: production, characterization, and immunodiagnostic application.

Authors:  F Qadri; M G Mohi; A Chowdhury; K Alam; T Azim; C Sears; R B Sack; M J Albert
Journal:  Clin Diagn Lab Immunol       Date:  1996-09

4.  Cloning and characterization of the gene for the metalloprotease enterotoxin of Bacteroides fragilis.

Authors:  J J Kling; R L Wright; J S Moncrief; T D Wilkins
Journal:  FEMS Microbiol Lett       Date:  1997-01-15       Impact factor: 2.742

5.  Production of active Serratia marcescens metalloprotease from Escherichia coli by alpha-hemolysin HlyB and HlyD.

Authors:  Y Suh; M J Benedik
Journal:  J Bacteriol       Date:  1992-04       Impact factor: 3.490

6.  The enterotoxin of Bacteroides fragilis is a metalloprotease.

Authors:  J S Moncrief; R Obiso; L A Barroso; J J Kling; R L Wright; R L Van Tassell; D M Lyerly; T D Wilkins
Journal:  Infect Immun       Date:  1995-01       Impact factor: 3.441

7.  Proteolytic activity of the Bacteroides fragilis enterotoxin causes fluid secretion and intestinal damage in vivo.

Authors:  R J Obiso; D M Lyerly; R L Van Tassell; T D Wilkins
Journal:  Infect Immun       Date:  1995-10       Impact factor: 3.441

8.  Isolation of enterotoxigenic Bacteroides fragilis from Bangladeshi children with diarrhea: a controlled study.

Authors:  R B Sack; M J Albert; K Alam; P K Neogi; M S Akbar
Journal:  J Clin Microbiol       Date:  1994-04       Impact factor: 5.948

9.  Human colonic epithelial cells, HT29/C1, treated with crude Bacteroides fragilis enterotoxin dramatically alter their morphology.

Authors:  C S Weikel; F D Grieco; J Reuben; L L Myers; R B Sack
Journal:  Infect Immun       Date:  1992-02       Impact factor: 3.441

10.  Detection of intestinal and extra-intestinal strains of enterotoxigenic Bacteroides fragilis by the HT-29 cytotoxicity assay.

Authors:  A Pantosti; M Cerquetti; R Colangeli; F D'Ambrosio
Journal:  J Med Microbiol       Date:  1994-09       Impact factor: 2.472
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