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Literature DB >> 886646

Methyl group analysis of virion-associated high-molecular-weight RNA synthesized in vitro by purified vaccinia virus.

Abstract

The methylation pattern of virion-associated high-molecular-weight RNA synthesized in vitro by purified vaccinia virus has been determined. Analysis of purified high-molecular-weight RNA synthesized with S-[methyl-3H]-adenosylmethionine and alpha[32P]UTP as precursors gave the following results. (i) Eessentially all molecules contained blocked and methylated structures of the type m7G(5')ppp(5')Gm and m7G(5')ppp(5')Am. (ii) There was no detectable methylation at internal sites. (iii) Under several different conditions of synthesis, the ratio of molecules containing m7G(5')ppp(5')Gm to those containing m7G(5')ppp(5')Am was imilar for both the virion-associated high-molecular-weight RNA and the virion-released 8-12S mRNA.

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Year: 1977 PMID： 886646 PMCID： PMC515806

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

22 in total

4. Soluble endoribonuclease activity from vaccinia virus: specific cleavage of virion-associated high-molecular-weight RNA.

Authors: E Paoletti; B R Lipinskas
Journal: J Virol Date: 1978-06 Impact factor: 5.103

5. Capped and polyadenylated low-molecular-weight RNA synthesized by vaccinia virus in vitro.

Authors: E Paoletti; B R Lipinskas; D Panicali
Journal: J Virol Date: 1980-01 Impact factor: 5.103

6. Analysis of vaccinia virus transcriptional complexity in vitro and in vivo: characterization of RNase T1-resistant 5'-terminal oligonucleotides.

Authors: C Whitkop; B R Lipinskas; S Mercer; D Panicali; E Paoletti
Journal: J Virol Date: 1982-05 Impact factor: 5.103

7. Abortive transcription products of vaccinia virus are guanylylated, methylated, and polyadenylylated.

Authors: A Gershowitz; B Moss
Journal: J Virol Date: 1979-09 Impact factor: 5.103

7 in total

Methyl group analysis of virion-associated high-molecular-weight RNA synthesized in vitro by purified vaccinia virus.

1. Detection in HeLa cell extracts of a 7-methyl guanosine specific enzyme activity that cleaves m7GpppNm.

2. 5'-Terminal m-7G(5')ppp(5')G-m-p in vivo: identification in reovirus genome RNA.

3. Methylated nucleotides block 5'-terminus of vaccinia virus messenger RNA.

4. The modified 5'-terminal sequences in messenger RNA of mouse myeloma cells.

5. Methyl labeling of HeLa cell hnRNA: a comparison with mRNA.

6. Molecular weights of some HeLa ribosomal RNA's.

7. The effects of methionine deprivation on ribosome synthesis in HeLa cells.

8. Purification of mRNA guanylyltransferase and mRNA (guanine-7-) methyltransferase from vaccinia virions.

9. Ribonucleic acid synthesis in vaccinia virus. I. The mechanism of synthesis and release of RNA in vaccinia cores.

10. Modification of the 5'-terminus of mRNA by soluble guanylyl and methyl transferases from vaccinia virus.

1. Cell-free translation of purified virion-associated high-molecular-weight RNA synthesized in vitro by vaccinia virus.

2. In vitro synthesis and modification of mRNA by exvectorial isolates of wound tumor virus.

3. Transcriptional complexity of vaccinia virus in vivo and in vitro.

4. Soluble endoribonuclease activity from vaccinia virus: specific cleavage of virion-associated high-molecular-weight RNA.

5. Capped and polyadenylated low-molecular-weight RNA synthesized by vaccinia virus in vitro.

6. Analysis of vaccinia virus transcriptional complexity in vitro and in vivo: characterization of RNase T1-resistant 5'-terminal oligonucleotides.

7. Abortive transcription products of vaccinia virus are guanylylated, methylated, and polyadenylylated.