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Literature DB >> 1058472

Modification of the 5'-terminus of mRNA by soluble guanylyl and methyl transferases from vaccinia virus.

M J Ensinger, S A Martin, E Paoletti, B Moss.

Abstract

RNA guanylyl and methyl transferases have been solubilized from vaccinia virus cores. The guanylyl transferase specifically adds a GMP residue to the 5'-terminus of unmethylated vaccinia virus mRNA to form the structures G(5')ppp(5')Gp- and G(5')ppp(5')Ap-. Studies with [alpha-32P]GTP and [beta, gamma-32P]GTP indicated that only the alpha-phosphate is transferred. In the presence of S-adenosylmethionine, the methyl transferases convert the blocked 5'-termini to m7G(5')ppp(5')Gmp- and m7G(5')ppp(5')Amp-. Similarly, the enzymes can modify synthetic poly(A) to form the structure m7G(5')ppp(5')Amp-.

Entities: Chemical Gene Species

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Year: 1975 PMID： 1058472 PMCID： PMC432801 DOI： 10.1073/pnas.72.7.2525

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

19 in total

9. Vaccinia virus early gene transcription termination factors VTF and Rap94 interact with the U9 termination motif in the nascent RNA in a transcription ternary complex.

Authors: Linda A Christen; Sarah Piacente; Mohamed R Mohamed; Edward G Niles
Journal: Virology Date: 2008-05-01 Impact factor: 3.616

10. Synthesis of polynucleotide 5'-triphosphatase in vaccinia virus-infected HeLa cells.

Authors: D J Tutas; E Paoletti
Journal: J Virol Date: 1978-01 Impact factor: 5.103

Modification of the 5'-terminus of mRNA by soluble guanylyl and methyl transferases from vaccinia virus.

1. 5'-Terminal m-7G(5')ppp(5')G-m-p in vivo: identification in reovirus genome RNA.

2. Methylated nucleotides block 5'-terminus of vaccinia virus messenger RNA.

3. Protein measurement with the Folin phenol reagent.

4. Identification of the nucleoside monophosphate end-group on the product of the polynucleotide phosphorylase reaction.

5. Polyadenylate polymerase from vaccinia virions.

6. Single-stranded deoxyribonucleic acid-specific nuclease from vaccinia virus. Purification and characterization.

7. Transfer ribonucleic acid methylases of HeLa cells.

8. A new chemical procedure for 32P-labeling of ribonucleic acids at their 5'-ends after isolation.

9. Ribonucleic acid synthesis in vaccinia virus. I. The mechanism of synthesis and release of RNA in vaccinia cores.

10. Methylation of newly synthesized viral messenger RNA by an enzyme in vaccinia virus.

Review 1. An unconventional pathway of mRNA cap formation by vesiculoviruses.

Review 2. In vitro capping and transcription of rhabdoviruses.

3. Cell-free translation of purified virion-associated high-molecular-weight RNA synthesized in vitro by vaccinia virus.

4. Cytological evidence for switches in polarity of chromosomal DNA.

5. Blocked 5' termini in brome mosaic virus RNA.

6. Phenotypic analysis of a temperature sensitive mutant in the large subunit of the vaccinia virus mRNA capping enzyme.

7. Regulation of Sendai virus transcription: evidence for a single promoter in vivo.

8. Two methyltransferase activities in the purified virions of vesicular stomatitis virus.

9. Vaccinia virus early gene transcription termination factors VTF and Rap94 interact with the U9 termination motif in the nascent RNA in a transcription ternary complex.

10. Synthesis of polynucleotide 5'-triphosphatase in vaccinia virus-infected HeLa cells.