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Literature DB >> 885031

Biochemical analysis of meiosis in the male mouse. II. DNA metabolism at pachytene.

Abstract

The DNA metabolism of mouse spermatogenic cells was investigated by intravenous administration of isotope and Staput fractionation of the cells. The pattern of metabolism is virtually identical with that observed in Lilium microsporocytes. A programmed single strand nicking of DNA occure at pachytene such that at least 50% of the DNA is in the form of 62S fragments. Repair replication of endogenously nicked sites is fully achieved during pachytene. The sites of nicking and repair are preferentially located in sequences that are repeated about 400 times. These results are considered as strong evidence for a universal pattern of meiotic prophase DNA metabolism which is associated with crossing-over.

Entities: Species

Mesh：

Substances：
DNA, Single-Stranded
DNA

Year: 1977 PMID： 885031 DOI： 10.1007/bf00286047

Source DB: PubMed Journal: Chromosoma ISSN： 0009-5915 Impact factor: 4.316

12 in total

1. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA.

Authors: F W STUDIER
Journal: J Mol Biol Date: 1965-02 Impact factor: 5.469

2. Anomalies in sedimentation. V. Chains at high fields, practical consequences.

Authors: B H Zimm; V N Schumaker; C B Zimm
Journal: Biophys Chem Date: 1976-07 Impact factor: 2.352

Review 3. Biochemical controls of meiosis.

Authors: H Stern; Y Hotta
Journal: Annu Rev Genet Date: 1973 Impact factor: 16.830

4. Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms.

Authors: R J Britten; D E Kohne
Journal: Science Date: 1968-08-09 Impact factor: 47.728

5. DNA sequence interspersion and a speculation about evolution.

Authors: B J Britten
Journal: Brookhaven Symp Biol Date: 1972

6. Radiation-initiated DNA synthesis in spermatogenic cells of the mouse.

Authors: S Kofman-Alfaro; A C Chandley
Journal: Exp Cell Res Date: 1971-11 Impact factor: 3.905

7. Single-strand scissions of chromosomal DNA during commitment to recombination at meiosis.

Authors: G K Jacobson; R Pinon; R E Esposito; M S Esposito
Journal: Proc Natl Acad Sci U S A Date: 1975-05 Impact factor: 11.205

8. Meiotic DNA synthesis during mouse spermatogenesis.

Authors: M L Meistrich; B O Reid; W J Barcellona
Journal: J Cell Biol Date: 1975-01 Impact factor: 10.539

9. Biochemistry of meiosis.

Authors: H Stern; Y Hotta
Journal: Philos Trans R Soc Lond B Biol Sci Date: 1977-03-21 Impact factor: 6.237

10. In vivo BrdU-33258 Hoechst analysis of DNA replication kinetics and sister chromatid exchange formation in mouse somatic and meiotic cells.

Authors: J W Allen; S A Latt
Journal: Chromosoma Date: 1976-11-29 Impact factor: 4.316

16 in total

1. A disruption of pachytene DNA metabolism in male mice with chromosomally-derived sterility.

Authors: Y Hotta; A C Chandley; H Stern; A G Searle; C V Beechey
Journal: Chromosoma Date: 1979-08 Impact factor: 4.316

Review 2. The organization of DNA metabolism during the recombinational phase of meiosis with special reference to humans.

Authors: H Stern; Y Hotta
Journal: Mol Cell Biochem Date: 1980-02-28 Impact factor: 3.396

Biochemical analysis of meiosis in the male mouse. II. DNA metabolism at pachytene.

1. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA.

2. Anomalies in sedimentation. V. Chains at high fields, practical consequences.

Review 3. Biochemical controls of meiosis.

4. Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms.

5. DNA sequence interspersion and a speculation about evolution.

6. Radiation-initiated DNA synthesis in spermatogenic cells of the mouse.

7. Single-strand scissions of chromosomal DNA during commitment to recombination at meiosis.

8. Meiotic DNA synthesis during mouse spermatogenesis.

9. Biochemistry of meiosis.

10. In vivo BrdU-33258 Hoechst analysis of DNA replication kinetics and sister chromatid exchange formation in mouse somatic and meiotic cells.

1. A disruption of pachytene DNA metabolism in male mice with chromosomally-derived sterility.

Review 2. The organization of DNA metabolism during the recombinational phase of meiosis with special reference to humans.

3. Biochemical analysis of meiosis in the male mouse. I. Separation of DNA labelling of specific spermatogenic stages.

4. Information theory, gene expression, and combinatorial regulation: a quantitative analysis.

5. Expression of the mouse testicular histone gene H1t during spermatogenesis.

6. Regions of active chromatin conformation in 'inactive' male meiotic sex chromosomes of the mouse.

7. Meiotic effects of DNA-defective cell division cycle mutations of Saccharomyces cerevisiae.

8. Human male infertility, probably genetically determined, due to defective meiosis and spermatogenic arrest.

9. Absence of satellite DNA synthesis during meiotic prophase in mouse and human spermatocytes.

10. Genetically determined asynapsis, spermatogenic degeneration, and infertility in men.