Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Biochemical analysis of meiosis in the male mouse. I. Separation of DNA labelling of specific spermatogenic stages.

Literature DB >> 885030

Biochemical analysis of meiosis in the male mouse. I. Separation of DNA labelling of specific spermatogenic stages.

Abstract

Spermatogenic cells of the mouse have been separated by gravity sedimentation using a modification of a previously published method. Details are given for the collection of purified samples of specific meiotic stages and for the collection of labelled cell fractions following injection of 3H-thymidine. Suppression of semi-conservative meiotic DNA synthesis, essential to the biochemical analysis of pachytene DNA metabolism, has been achieved by in vivo administration of 1 M hydroxyurea.

Entities: Chemical Species

Mesh：

Substances：

Year: 1977 PMID： 885030 DOI： 10.1007/bf00286046

Source DB: PubMed Journal: Chromosoma ISSN： 0009-5915 Impact factor: 4.316

6 in total

1. Separation of mouse spermatogenic cells by sedimentation velocity. A morphological characterization.

Authors: L J Romrell; A R Bellvé; D W Fawcett
Journal: Dev Biol Date: 1976-03 Impact factor: 3.582

2. Biochemical analysis of meiosis in the male mouse. II. DNA metabolism at pachytene.

Authors: Y Hotta; A C Chandley; H Stern
Journal: Chromosoma Date: 1977-07-08 Impact factor: 4.316

3. Separation of mouse spermatogenic cells by velocity sedimentation.

Authors: M L Meistrich
Journal: J Cell Physiol Date: 1972-10 Impact factor: 6.384

4. Separation of cells by velocity sedimentation.

Authors: R G Miller; R A Phillips
Journal: J Cell Physiol Date: 1969-06 Impact factor: 6.384

5. The separation, physical characterization, and differentiation kinetics of spermatogonial cells of the mouse.

Authors: D M Lam; R Furrer; W R Bruce
Journal: Proc Natl Acad Sci U S A Date: 1970-01 Impact factor: 11.205

6. The occurrence of intercellular bridges in groups of cells exhibiting synchronous differentiation.

Authors: D W FAWCETT; S ITO; D SLAUTTERBACK
Journal: J Biophys Biochem Cytol Date: 1959-05-25

6 in total

15 in total

1. A disruption of pachytene DNA metabolism in male mice with chromosomally-derived sterility.

Authors: Y Hotta; A C Chandley; H Stern; A G Searle; C V Beechey
Journal: Chromosoma Date: 1979-08 Impact factor: 4.316

2. DNase I-hypersensitive sites and transcription factor-binding motifs within the mouse E beta meiotic recombination hot spot.

Authors: R Shenkar; M H Shen; N Arnheim
Journal: Mol Cell Biol Date: 1991-04 Impact factor: 4.272

Review 3. The organization of DNA metabolism during the recombinational phase of meiosis with special reference to humans.

Authors: H Stern; Y Hotta
Journal: Mol Cell Biochem Date: 1980-02-28 Impact factor: 3.396

4. Biochemical analysis of meiosis in the male mouse. II. DNA metabolism at pachytene.

Authors: Y Hotta; A C Chandley; H Stern
Journal: Chromosoma Date: 1977-07-08 Impact factor: 4.316

5. DNA synthesis at selective sites during pachytene in mouse spermatocytes.

Authors: L Stubbs; H Stern
Journal: Chromosoma Date: 1986 Impact factor: 4.316

6. Developmentally regulated expression of a human "finger"-containing gene encoded by the 5' half of the ret transforming gene.

Authors: M Takahashi; Y Inaguma; H Hiai; F Hirose
Journal: Mol Cell Biol Date: 1988-04 Impact factor: 4.272

10. Aged mouse ovaries possess rare premeiotic germ cells that can generate oocytes following transplantation into a young host environment.

Authors: Yuichi Niikura; Teruko Niikura; Jonathan L Tilly
Journal: Aging (Albany NY) Date: 2009-12-12 Impact factor: 5.682