Literature DB >> 8836196

Rapid preparation of single stranded DNA from PCR products by streptavidin induced electrophoretic mobility shift.

N C Pagratis1.   

Abstract

Streptavidin induced electrophoretic mobility shift was used to prepare single stranded (ss) DNA amplified with the polymerase chain reaction in the presence of a biotinylated and a non-biotinylated primer. A variety of denaturing conditions, including incubation at 95 degrees C in 50% formamide can be used without disrupting the streptavidin-biotinylated-ssDNA complex. Following electrophoresis, pure non-biotinylated DNA can be efficiently recovered from 7 M urea gels because it is well separated from the severely retarded streptavidin-biotinylated-ssDNA complex. Quantitative complexing of biotinylated ssDNA can occur at a streptavidin to DNA molar ratio of 1 or more.

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Year:  1996        PMID： 8836196      PMCID： PMC146126          DOI： 10.1093/nar/24.18.3645

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  9 in total

1.  Direct single stranded sequencing from agarose of polymerase chain reaction products.

Authors:  A Green; A Roopra; M Vaudin
Journal:  Nucleic Acids Res       Date:  1990-10-25       Impact factor: 16.971

2.  Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase.

Authors:  C Tuerk; L Gold
Journal:  Science       Date:  1990-08-03       Impact factor: 47.728

3.  Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support.

Authors:  T Hultman; S Ståhl; E Hornes; M Uhlén
Journal:  Nucleic Acids Res       Date:  1989-07-11       Impact factor: 16.971

4.  Efficient production of single-stranded DNA as long as 2 kb for sequencing of PCR-amplified DNA.

Authors:  B Kaltenboeck; J W Spatafora; X Zhang; K G Kousoulas; M Blackwell; J Storz
Journal:  Biotechniques       Date:  1992-02       Impact factor: 1.993

5.  Production of single-stranded DNA templates by exonuclease digestion following the polymerase chain reaction.

Authors:  R G Higuchi; H Ochman
Journal:  Nucleic Acids Res       Date:  1989-07-25       Impact factor: 16.971

6.  Affinity generation of single-stranded DNA for dideoxy sequencing following the polymerase chain reaction.

Authors:  L G Mitchell; C R Merril
Journal:  Anal Biochem       Date:  1989-05-01       Impact factor: 3.365

7.  PCR product with strands of unequal length.

Authors:  K P Williams; D P Bartel
Journal:  Nucleic Acids Res       Date:  1995-10-25       Impact factor: 16.971

8.  Detection of bacteriophage phi 6 minus-strand RNA and novel mRNA isoconformers synthesized in vivo and in vitro, by strand-separating agarose gels.

Authors:  N Pagratis; H R Revel
Journal:  Virology       Date:  1990-07       Impact factor: 3.616

9.  Synthesis of PCR-derived, single-stranded DNA probes suitable for in situ hybridization.

Authors:  K Hannon; E Johnstone; L S Craft; S P Little; C K Smith; M L Heiman; R F Santerre
Journal:  Anal Biochem       Date:  1993-08-01       Impact factor: 3.365
  9 in total
  12 in total

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2.  Analysis of single nucleic acid molecules with protein nanopores.

Authors:  Giovanni Maglia; Andrew J Heron; David Stoddart; Deanpen Japrung; Hagan Bayley
Journal:  Methods Enzymol       Date:  2010       Impact factor: 1.600

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Authors:  Tulsi Ram Damase; Andrew D Ellington; Peter B Allen
Journal:  Biotechniques       Date:  2017-06-01       Impact factor: 1.993

4.  An Aptamer against the Matrix Binding Domain on the Hepatitis B Virus Capsid Impairs Virion Formation.

Authors:  Ahmed Orabi; Maria Bieringer; Arie Geerlof; Volker Bruss
Journal:  J Virol       Date:  2015-07-01       Impact factor: 5.103

5.  Thermodynamically stable RNA three-way junction for constructing multifunctional nanoparticles for delivery of therapeutics.

Authors:  Dan Shu; Yi Shu; Farzin Haque; Sherine Abdelmawla; Peixuan Guo
Journal:  Nat Nanotechnol       Date:  2011-09-11       Impact factor: 39.213

6.  Aptamer mediated siRNA delivery.

Authors:  Ted C Chu; Karen Y Twu; Andrew D Ellington; Matthew Levy
Journal:  Nucleic Acids Res       Date:  2006-06-01       Impact factor: 16.971

7.  DNA display I. Sequence-encoded routing of DNA populations.

Authors:  David R Halpin; Pehr B Harbury
Journal:  PLoS Biol       Date:  2004-06-22       Impact factor: 8.029

8.  Crystal structure of 3WJ core revealing divalent ion-promoted thermostability and assembly of the Phi29 hexameric motor pRNA.

Authors:  Hui Zhang; James A Endrizzi; Yi Shu; Farzin Haque; Claude Sauter; Lyudmila S Shlyakhtenko; Yuri Lyubchenko; Peixuan Guo; Young-In Chi
Journal:  RNA       Date:  2013-07-24       Impact factor: 4.942

9.  Upgrading SELEX technology by using lambda exonuclease digestion for single-stranded DNA generation.

Authors:  Meltem Avci-Adali; Angel Paul; Nadj Wilhelm; Gerhard Ziemer; Hans Peter Wendel
Journal:  Molecules       Date:  2009-12-24       Impact factor: 4.411

10.  cDNA display: a novel screening method for functional disulfide-rich peptides by solid-phase synthesis and stabilization of mRNA-protein fusions.

Authors:  Junichi Yamaguchi; Mohammed Naimuddin; Manish Biyani; Toru Sasaki; Masayuki Machida; Tai Kubo; Takashi Funatsu; Yuzuru Husimi; Naoto Nemoto
Journal:  Nucleic Acids Res       Date:  2009-06-15       Impact factor: 16.971
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