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Literature DB >> 2235520

Direct single stranded sequencing from agarose of polymerase chain reaction products.

A Green¹, A Roopra, M Vaudin.

Abstract

Entities: Chemical

Mesh：

Substances：

Year: 1990 PMID： 2235520 PMCID： PMC332449 DOI： 10.1093/nar/18.20.6163

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

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6 in total

1. Optimal conditions for directly sequencing double-stranded PCR products with sequenase.

Authors: J L Casanova; C Pannetier; C Jaulin; P Kourilsky
Journal: Nucleic Acids Res Date: 1990-07-11 Impact factor: 16.971

2. A rapid semi-automated microtiter plate method for analysis and sequencing by PCR from bacterial stocks.

Authors: J P Schofield; M Vaudin; S Kettle; D S Jones
Journal: Nucleic Acids Res Date: 1989-11-25 Impact factor: 16.971

3. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

Authors: R K Saiki; D H Gelfand; S Stoffel; S J Scharf; R Higuchi; G T Horn; K B Mullis; H A Erlich
Journal: Science Date: 1988-01-29 Impact factor: 47.728

4. Direct sequencing from low-melt agarose with Sequenase.

Authors: K A Kretz; G S Carson; J S O'Brien
Journal: Nucleic Acids Res Date: 1989-07-25 Impact factor: 16.971

5. Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus.

Authors: U B Gyllensten; H A Erlich
Journal: Proc Natl Acad Sci U S A Date: 1988-10 Impact factor: 11.205

6. An improved method for directly sequencing PCR amplified material using dimethyl sulphoxide.

Authors: P R Winship
Journal: Nucleic Acids Res Date: 1989-02-11 Impact factor: 16.971

6 in total

4 in total

Direct single stranded sequencing from agarose of polymerase chain reaction products.

1. Optimal conditions for directly sequencing double-stranded PCR products with sequenase.

2. A rapid semi-automated microtiter plate method for analysis and sequencing by PCR from bacterial stocks.

3. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

4. Direct sequencing from low-melt agarose with Sequenase.

5. Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus.

6. An improved method for directly sequencing PCR amplified material using dimethyl sulphoxide.

1. cDNA cloning of 5' terminal regions.

2. Rapid preparation of single stranded DNA from PCR products by streptavidin induced electrophoretic mobility shift.

3. Sequencing of double-stranded PCR products.

Review 4. DNA sequencing. Recent innovations and future trends.