Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 A general ribonuclease assay using methylene blue.

Literature DB >> 8811875

A general ribonuclease assay using methylene blue.

Abstract

Ribonuclease (RNase) activity has been assayed by monitoring the shift in the absorbance maximum of methylene blue upon intercalation into high-molecular-weight RNA. After preincubation of yeast RNA with methylene blue, the initial rate of hydrolysis can be followed spectrophotometrically at a wavelength of 688 nm. This allows enzyme kinetic studies of RNases with their probable native substrate, RNA, in place of the artificial ones, such as dinucleoside phosphates or cyclic monophosphates, currently used.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 1996 PMID： 8811875 DOI： 10.1006/abio.1996.0326

Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365

Keyword Cloud
Cited

11 in total

1. Binding patterns and kinetics of RNase a interaction with RNA.

Authors: S Safarian; A A Moosavi-Movahedi
Journal: J Protein Chem Date: 2000-07

2. AtHESPERIN: a novel regulator of circadian rhythms with poly(A)-degrading activity in plants.

Authors: Costas Delis; Afrodite Krokida; Anastasia Tomatsidou; Daniela Tsikou; Rafailia A A Beta; Maria Tsioumpekou; Julietta Moustaka; Georgios Stravodimos; Demetres D Leonidas; Nikolaos A A Balatsos; Kalliope K Papadopoulou
Journal: RNA Biol Date: 2016 Impact factor: 4.652

3. Biological activities of the antiviral protein BE27 from sugar beet (Beta vulgaris L.).

Authors: Rosario Iglesias; Lucía Citores; Antimo Di Maro; José M Ferreras
Journal: Planta Date: 2014-10-19 Impact factor: 4.116

4. The antibacterial prodrug activator Rv2466c is a mycothiol-dependent reductase in the oxidative stress response of Mycobacterium tuberculosis.

Authors: Leonardo Astolfi Rosado; Khadija Wahni; Giulia Degiacomi; Brandán Pedre; David Young; Alfonso G de la Rubia; Francesca Boldrin; Edo Martens; Laura Marcos-Pascual; Enea Sancho-Vaello; David Albesa-Jové; Roberta Provvedi; Charlotte Martin; Vadim Makarov; Wim Versées; Guido Verniest; Marcelo E Guerin; Luis M Mateos; Riccardo Manganelli; Joris Messens
Journal: J Biol Chem Date: 2017-06-15 Impact factor: 5.157

5. Methanosarcina acetivorans utilizes a single NADPH-dependent thioredoxin system and contains additional thioredoxin homologues with distinct functions.

Authors: Addison C McCarver; Faith H Lessner; Jose M Soroeta; Daniel J Lessner
Journal: Microbiology Date: 2017-02-08 Impact factor: 2.777

10. A novel mycothiol-dependent thiol-disulfide reductase in Corynebacterium glutamicum involving oxidative stress resistance.

Authors: Yang Liu; Xiaona Li; Jiaxin Luo; Tao Su; Meiru Si; Can Chen
Journal: 3 Biotech Date: 2021-07-14 Impact factor: 2.406

A general ribonuclease assay using methylene blue.

1. Binding patterns and kinetics of RNase a interaction with RNA.

2. AtHESPERIN: a novel regulator of circadian rhythms with poly(A)-degrading activity in plants.

3. Biological activities of the antiviral protein BE27 from sugar beet (Beta vulgaris L.).

4. The antibacterial prodrug activator Rv2466c is a mycothiol-dependent reductase in the oxidative stress response of Mycobacterium tuberculosis.

5. Methanosarcina acetivorans utilizes a single NADPH-dependent thioredoxin system and contains additional thioredoxin homologues with distinct functions.

6. A deadenylase assay by size-exclusion chromatography.

7. Converting a Sulfenic Acid Reductase into a Disulfide Bond Isomerase.

8. A fluorescence-based assay suitable for quantitative analysis of deadenylase enzyme activity.

9. A hybrid chimeric system for versatile and ultra-sensitive RNase detection.

10. A novel mycothiol-dependent thiol-disulfide reductase in Corynebacterium glutamicum involving oxidative stress resistance.