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Literature DB >> 8811099

In vivo analysis of the plasmid pAM beta 1 resolution system.

L Jannière¹, S McGovern, C Pujol, M A Petit, S D Ehrlich.

Abstract

The promiscuous plasmid pAM beta 1 from Gram-positive bacteria encodes a resolution system which differs from that of Tn3 in that (i) it requires a histone-like protein and an unusual resolvase-DNA interaction to promote recombination and (ii) it mediates in vivo DNA inversion in plasmid substrates. In this in vivo analysis, the pAM beta 1 resolution site is narrowed down to a 99 bp segment, the strand exchange is mapped within 10 bp and the serine residue at position 10 of the resolvase is shown to be essential for enzyme activity. In addition, data showing that the resolution system does not promote DNA inversion in the Bacillus subtilis chromosome are presented. Implications of this observation are discussed.

Entities: Chemical Species

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Year: 1996 PMID： 8811099 PMCID： PMC146103 DOI： 10.1093/nar/24.17.3431

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

33 in total

1. Recombination of knotted substrates by Tn3 resolvase.

Authors: P Dröge; N R Cozzarelli
Journal: Proc Natl Acad Sci U S A Date: 1989-08 Impact factor: 11.205

2. DNA supercoiling determines the activation energy barrier for site specific recombination by Tn21 resolvase.

Authors: S E Castell; S E Halford
Journal: Nucleic Acids Res Date: 1989-09-12 Impact factor: 16.971

3. Recombination between short repeated sequences is more frequent in plasmids than in the chromosome of Bacillus subtilis.

Authors: L Jannière; S D Ehrlich
Journal: Mol Gen Genet Date: 1987-11

4. Recombination site selection by Tn3 resolvase: topological tests of a tracking mechanism.

Authors: H W Benjamin; M M Matzuk; M A Krasnow; N R Cozzarelli
Journal: Cell Date: 1985-01 Impact factor: 41.582

5. Processing of a sporulation sigma factor in Bacillus subtilis: how morphological structure could control gene expression.

Authors: P Stragier; C Bonamy; C Karmazyn-Campelli
Journal: Cell Date: 1988-03-11 Impact factor: 41.582

In vivo analysis of the plasmid pAM beta 1 resolution system.

1. Recombination of knotted substrates by Tn3 resolvase.

2. DNA supercoiling determines the activation energy barrier for site specific recombination by Tn21 resolvase.

3. Recombination between short repeated sequences is more frequent in plasmids than in the chromosome of Bacillus subtilis.

4. Recombination site selection by Tn3 resolvase: topological tests of a tracking mechanism.

5. Processing of a sporulation sigma factor in Bacillus subtilis: how morphological structure could control gene expression.

6. The tyrosine-6 hydroxyl of gamma delta resolvase is not required for the DNA cleavage and rejoining reactions.

7. Enhancer-independent mutants of the Cin recombinase have a relaxed topological specificity.

8. Isolation and characterization of the Tn3 resolvase synaptic intermediate.

9. Characterization of the staphylococcal beta-lactamase transposon Tn552.

10. Isolation and characterization of unusual gin mutants.

1. Site-specific DNA Inversion by Serine Recombinases.

2. Novel shuttle vector pGMβ1 for conjugative chromosomal manipulation of Lactobacillus delbrueckii subsp. bulgaricus.