Literature DB >> 8804408

Gene transfer and manipulation in the thermophilic cyanobacterium Synechococcus elongatus.

U Mühlenhoff1, F Chauvat.   

Abstract

DNA can be introduced into the thermophilic cyanobacterium Synechococcus elongatus by electroporation or conjugation. Its genome can be readily manipulated through integrative transformation or by using promiscuous RSF1010-derived plasmids that can be transferred unaltered between Escherichia coli and Synechococcus elongatus. These vectors can therefore be used for in vivo studies of cyanobacterial proteins in both mesophilic and thermophilic cyanobacterial backgrounds. As a preliminary step towards the analysis of structure-function relationships of photosystem I (PSI) from this thermophile, the genes encoding the PSI subunits PsaF, PsaL, and PsaK were inactivated and shown to be non-essential in S. elongatus. In addition, PSI reaction centres were extracted from a psaL- strain exclusively as monomeric complexes.

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Year:  1996        PMID： 8804408     DOI： 10.1007/bf02173209

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  22 in total

1.  Nucleotide sequences of the psaA and psaB genes encoding the photosystem I core proteins from the thermophilic cyanobacterium Synechococcus vulcanus.

Authors:  T Shimizu; T Hiyama; M Ikeuchi; Y Inoue
Journal:  Plant Mol Biol       Date:  1992-02       Impact factor: 4.076

2.  Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa.

Authors:  H Schägger; G von Jagow
Journal:  Anal Biochem       Date:  1987-11-01       Impact factor: 3.365

3.  Identification of photosystem I components from the cyanobacterium, Synechococcus vulcanus by N-terminal sequencing.

Authors:  H Koike; M Ikeuchi; T Hiyama; Y Inoue
Journal:  FEBS Lett       Date:  1989-08-14       Impact factor: 4.124

4.  Transformation of a filamentous cyanobacterium by electroporation.

Authors:  T Thiel; H Poo
Journal:  J Bacteriol       Date:  1989-10       Impact factor: 3.490

5.  Molecular characterization of phycobilisome regulatory mutants of Fremyella diplosiphon.

Authors:  B U Bruns; W R Briggs; A R Grossman
Journal:  J Bacteriol       Date:  1989-02       Impact factor: 3.490

6.  PsaL subunit is required for the formation of photosystem I trimers in the cyanobacterium Synechocystis sp. PCC 6803.

Authors:  V P Chitnis; P R Chitnis
Journal:  FEBS Lett       Date:  1993-12-27       Impact factor: 4.124

7.  Site-directed mutagenesis in photosystem II of the cyanobacterium Synechocystis sp. PCC 6803: Donor D is a tyrosine residue in the D2 protein.

Authors:  W F Vermass; A W Rutherford; O Hansson
Journal:  Proc Natl Acad Sci U S A       Date:  1988-11       Impact factor: 11.205

8.  Targeted inactivation of the gene psaL encoding a subunit of photosystem I of the cyanobacterium Synechocystis sp. PCC 6803.

Authors:  V P Chitnis; Q Xu; L Yu; J H Golbeck; H Nakamoto; D L Xie; P R Chitnis
Journal:  J Biol Chem       Date:  1993-06-05       Impact factor: 5.157

9.  A conjugative plasmid vector for promoter analysis in several cyanobacteria of the genera Synechococcus and Synechocystis.

Authors:  P Marraccini; S Bulteau; C Cassier-Chauvat; P Mermet-Bouvier; F Chauvat
Journal:  Plant Mol Biol       Date:  1993-11       Impact factor: 4.076

10.  A versatile class of positive-selection vectors based on the nonviability of palindrome-containing plasmids that allows cloning into long polylinkers.

Authors:  J Elhai; C P Wolk
Journal:  Gene       Date:  1988-08-15       Impact factor: 3.688
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  21 in total

1.  Natural transformation of the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1: a simple and efficient method for gene transfer.

Authors:  K Onai; M Morishita; T Kaneko; S Tabata; M Ishiura
Journal:  Mol Genet Genomics       Date:  2003-11-25       Impact factor: 3.291

2.  Functional characterization and quantification of the alternative PsbA copies in Thermosynechococcus elongatus and their role in photoprotection.

Authors:  Julia Sander; Marc Nowaczyk; Joachim Buchta; Holger Dau; Imre Vass; Zsuzsanna Deák; Márta Dorogi; Masako Iwai; Matthias Rögner
Journal:  J Biol Chem       Date:  2010-07-27       Impact factor: 5.157

3.  Targeted inactivation of the psaK1, psaK2 and psaM genes encoding subunits of Photosystem I in the cyanobacterium Synechocystis sp. PCC 6803.

Authors:  S Naithani; J M Hou; P R Chitnis
Journal:  Photosynth Res       Date:  2000       Impact factor: 3.573

4.  Role of novel dimeric Photosystem II (PSII)-Psb27 protein complex in PSII repair.

Authors:  Nicole Grasse; Fikret Mamedov; Kristin Becker; Stenbjörn Styring; Matthias Rögner; Marc M Nowaczyk
Journal:  J Biol Chem       Date:  2011-07-07       Impact factor: 5.157

5.  Structural insights into a dimeric Psb27-photosystem II complex from a cyanobacterium Thermosynechococcus vulcanus.

Authors:  Guoqiang Huang; Yanan Xiao; Xiong Pi; Liang Zhao; Qingjun Zhu; Wenda Wang; Tingyun Kuang; Guangye Han; Sen-Fang Sui; Jian-Ren Shen
Journal:  Proc Natl Acad Sci U S A       Date:  2021-02-02       Impact factor: 11.205

6.  Lateral Segregation of Photosystem I in Cyanobacterial Thylakoids.

Authors:  Craig MacGregor-Chatwin; Melih Sener; Samuel F H Barnett; Andrew Hitchcock; Meghan C Barnhart-Dailey; Karim Maghlaoui; James Barber; Jerilyn A Timlin; Klaus Schulten; C Neil Hunter
Journal:  Plant Cell       Date:  2017-03-31       Impact factor: 11.277

7.  Plasmid conjugation from proteobacteria as evidence for the origin of xenologous genes in cyanobacteria.

Authors:  David Encinas; M Pilar Garcillán-Barcia; María Santos-Merino; Luis Delaye; Andrés Moya; Fernando de la Cruz
Journal:  J Bacteriol       Date:  2014-02-07       Impact factor: 3.490

8.  Functional characterization of the small regulatory subunit PetP from the cytochrome b6f complex in Thermosynechococcus elongatus.

Authors:  Sascha Rexroth; Dorothea Rexroth; Sebastian Veit; Nicole Plohnke; Kai U Cormann; Marc M Nowaczyk; Matthias Rögner
Journal:  Plant Cell       Date:  2014-08-19       Impact factor: 11.277

Review 9.  Biodesalination: a case study for applications of photosynthetic bacteria in water treatment.

Authors:  Jaime M Amezaga; Anna Amtmann; Catherine A Biggs; Tom Bond; Catherine J Gandy; Annegret Honsbein; Esther Karunakaran; Linda Lawton; Mary Ann Madsen; Konstantinos Minas; Michael R Templeton
Journal:  Plant Physiol       Date:  2014-03-07       Impact factor: 8.340

10.  Design and characterization of molecular tools for a Synthetic Biology approach towards developing cyanobacterial biotechnology.

Authors:  Hsin-Ho Huang; Daniel Camsund; Peter Lindblad; Thorsten Heidorn
Journal:  Nucleic Acids Res       Date:  2010-03-17       Impact factor: 16.971
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