Literature DB >> 2507531

Transformation of a filamentous cyanobacterium by electroporation.

T Thiel1, H Poo.   

Abstract

The filamentous cyanobacterium Anabaena sp. strain M131 was transformed with the shuttle vector pRL6 by electroporation. Optimum conditions for electroporation required relatively high field strengths with short time constants. Restriction significantly lowered the efficiency of transformation. A plasmid containing a single unmodified AvaII restriction site transformed cells with about 100-fold-lower efficiency than did the same plasmid with a modified restriction site.

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Year:  1989        PMID： 2507531      PMCID： PMC210424          DOI： 10.1128/jb.171.10.5743-5746.1989

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  14 in total

1.  Survey of extrachromosomal DNA found in the filamentous cyanobacteria.

Authors:  R D Simon
Journal:  J Bacteriol       Date:  1978-10       Impact factor: 3.490

2.  Studies on Nitrogen-Fixing Blue-Green Algae. I. Growth and Nitrogen Fixation by Anabaena Cylindrica Lemm.

Authors:  M B Allen; D I Arnon
Journal:  Plant Physiol       Date:  1955-07       Impact factor: 8.340

Review 3.  Transformation in cyanobacteria.

Authors:  R D Porter
Journal:  Crit Rev Microbiol       Date:  1986       Impact factor: 7.624

4.  Electroporation of eukaryotes and prokaryotes: a general approach to the introduction of macromolecules into cells.

Authors:  K Shigekawa; W J Dower
Journal:  Biotechniques       Date:  1988-09       Impact factor: 1.993

5.  Identification of facultatively heterotrophic, N2-fixing cyanobacteria able to receive plasmid vectors from Escherichia coli by conjugation.

Authors:  E Flores; C P Wolk
Journal:  J Bacteriol       Date:  1985-06       Impact factor: 3.490

Review 6.  Gaining access to the cytosol: the technique and some applications of electropermeabilization.

Authors:  D E Knight; M C Scrutton
Journal:  Biochem J       Date:  1986-03-15       Impact factor: 3.857

7.  Conjugal transfer of DNA to cyanobacteria.

Authors:  J Elhai; C P Wolk
Journal:  Methods Enzymol       Date:  1988       Impact factor: 1.600

8.  Conjugal transfer of plasmids to cyanobacteria.

Authors:  T Thiel; C P Wolk
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

9.  Genetic recombination of bacterial plasmid DNA. Analysis of the effect of recombination-deficient mutations on plasmid recombination.

Authors:  A A James; P T Morrison; R Kolodner
Journal:  J Mol Biol       Date:  1982-09-25       Impact factor: 5.469

10.  Construction of shuttle vectors capable of conjugative transfer from Escherichia coli to nitrogen-fixing filamentous cyanobacteria.

Authors:  C P Wolk; A Vonshak; P Kehoe; J Elhai
Journal:  Proc Natl Acad Sci U S A       Date:  1984-03       Impact factor: 11.205
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  12 in total

1.  Natural transformation of the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1: a simple and efficient method for gene transfer.

Authors:  K Onai; M Morishita; T Kaneko; S Tabata; M Ishiura
Journal:  Mol Genet Genomics       Date:  2003-11-25       Impact factor: 3.291

2.  Conjugative gene transfer in marine cyanobacteria: Synechococcus sp., Synechocystis sp. and Pseudanabaena sp.

Authors:  K Sode; M Tatara; H Takeyama; J G Burgess; T Matsunaga
Journal:  Appl Microbiol Biotechnol       Date:  1992-06       Impact factor: 4.813

3.  Reduction of conjugal transfer efficiency by three restriction activities of Anabaena sp. strain PCC 7120.

Authors:  J Elhai; A Vepritskiy; A M Muro-Pastor; E Flores; C P Wolk
Journal:  J Bacteriol       Date:  1997-03       Impact factor: 3.490

4.  Genetic transformation and mutagenesis via single-stranded DNA in the unicellular, diazotrophic cyanobacteria of the genus Cyanothece.

Authors:  Hongtao Min; Louis A Sherman
Journal:  Appl Environ Microbiol       Date:  2010-09-17       Impact factor: 4.792

5.  Gene transfer and manipulation in the thermophilic cyanobacterium Synechococcus elongatus.

Authors:  U Mühlenhoff; F Chauvat
Journal:  Mol Gen Genet       Date:  1996-08-27

6.  Characterization of a restriction barrier and electrotransformation of the cyanobacterium Nostoc PCC 7121.

Authors:  D P Moser; D Zarka; T Kallas
Journal:  Arch Microbiol       Date:  1993       Impact factor: 2.552

7.  A conjugative plasmid vector for promoter analysis in several cyanobacteria of the genera Synechococcus and Synechocystis.

Authors:  P Marraccini; S Bulteau; C Cassier-Chauvat; P Mermet-Bouvier; F Chauvat
Journal:  Plant Mol Biol       Date:  1993-11       Impact factor: 4.076

8.  Premethylation of foreign DNA improves integrative transformation efficiency in Synechocystis sp. strain PCC 6803.

Authors:  Bo Wang; Jianping Yu; Weiwen Zhang; Deirdre R Meldrum
Journal:  Appl Environ Microbiol       Date:  2015-10-09       Impact factor: 4.792

9.  A genetic manipulation system for oceanic cyanobacteria of the genus Synechococcus.

Authors:  B Brahamsha
Journal:  Appl Environ Microbiol       Date:  1996-05       Impact factor: 4.792

10.  Transformation and conjugal transfer of foreign genes into the filamentous multicellular cyanobacteria (subsection V) Fischerella and Chlorogloeopsis.

Authors:  Karina Stucken; Judith Ilhan; Mayo Roettger; Tal Dagan; William F Martin
Journal:  Curr Microbiol       Date:  2012-07-26       Impact factor: 2.188
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