OBJECTIVE: To examine the effect of human embryo coculture with an ovarian cancer cell line. DESIGN: Prospective, randomized in vitro study. SETTING: University of Toronto IVF clinic at The Toronto Hospital. PATIENTS: Couples undergoing IVF who chose not to cryopreserve their spare embryos and were willing to donate spare embryos for research. INTERVENTION: Spare embryos were cultured randomly either under regular conditions with Ham's F-10 medium supplemented with 10% heat inactivated human serum (n = 189) or were cocultured in the same medium, with human ovarian epithelial cancer cells (n = 173). MAIN OUTCOME MEASURE: Blastocyst formation. RESULTS: Coculture with the cancer cell line improved the preimplantation embryo development to the blastocyst stage. There was a significant increase in the number of cavitating morulae (68%) and the proportion of embryos reaching the fully expanded blastocyst stage (39%) compared with those in standard culture medium (34% and 23%, respectively). CONCLUSION: Coculture of early cleavage stage human embryos with epithelial cancer cells markedly improves in vitro human blastocyst formation compared with standard culture conditions.
OBJECTIVE: To examine the effect of human embryo coculture with an ovarian cancer cell line. DESIGN: Prospective, randomized in vitro study. SETTING: University of Toronto IVF clinic at The Toronto Hospital. PATIENTS: Couples undergoing IVF who chose not to cryopreserve their spare embryos and were willing to donate spare embryos for research. INTERVENTION: Spare embryos were cultured randomly either under regular conditions with Ham's F-10 medium supplemented with 10% heat inactivated human serum (n = 189) or were cocultured in the same medium, with humanovarian epithelial cancer cells (n = 173). MAIN OUTCOME MEASURE: Blastocyst formation. RESULTS: Coculture with the cancer cell line improved the preimplantation embryo development to the blastocyst stage. There was a significant increase in the number of cavitating morulae (68%) and the proportion of embryos reaching the fully expanded blastocyst stage (39%) compared with those in standard culture medium (34% and 23%, respectively). CONCLUSION: Coculture of early cleavage stage human embryos with epithelial cancer cells markedly improves in vitro humanblastocyst formation compared with standard culture conditions.
Authors: D T Carrell; C M Peterson; K P Jones; H H Hatasaka; L C Udoff; C E Cornwell; C Thorp; P Kuneck; L Erickson; B Campbell Journal: J Assist Reprod Genet Date: 1999-08 Impact factor: 3.412
Authors: R Fabbri; E Porcu; T Marsella; M R Primavera; S Cecconi; S A Nottola; P M Motta; S Venturoli; C Flamigni Journal: J Assist Reprod Genet Date: 2000-01 Impact factor: 3.412