Literature DB >> 8761446

Measurement of oxidized and methylated DNA bases by HPLC with electrochemical detection.

H Kaur1, B Halliwell.   

Abstract

Oxidative DNA damage is thought to be an important contributor to cancer development and to be affected by dietary constituents, so its accurate measurement is important. DNA methylation is recognized as an important mechanism affecting gene expression. In the present paper we describe an HPLC-with-electrochemical-detection procedure that allows rapid and sensitive measurement of four oxidized (2,6-diamino-4-hydroxy-5-formamidopyrimidine, 5-hydroxyuracil, 8-hydroxyguanine, 8-hydroxyadenine) and three methylated (7-methylguanine, 1-methylguanine, O6-methylguanine) bases in acid hydrolysates of DNA. Guanine was also detected, but was clearly separated from the other bases.

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Year:  1996        PMID： 8761446      PMCID： PMC1217582          DOI： 10.1042/bj3180021

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  25 in total

1.  Effects of formic acid hydrolysis on the quantitative analysis of radiation-induced DNA base damage products assayed by gas chromatography/mass spectrometry.

Authors:  S G Swarts; G S Smith; L Miao; K T Wheeler
Journal:  Radiat Environ Biophys       Date:  1996-02       Impact factor: 1.925

2.  A novel HPLC procedure for the analysis of 8-oxoguanine in DNA.

Authors:  K E Herbert; M D Evans; M T Finnegan; S Farooq; N Mistry; I D Podmore; P Farmer; J Lunec
Journal:  Free Radic Biol Med       Date:  1996       Impact factor: 7.376

Review 3.  The DNA methylation machinery as a target for anticancer therapy.

Authors:  M Szyf
Journal:  Pharmacol Ther       Date:  1996       Impact factor: 12.310

4.  Observation and prevention of an artefactual formation of oxidized DNA bases and nucleosides in the GC-EIMS method.

Authors:  T Douki; T Delatour; F Bianchini; J Cadet
Journal:  Carcinogenesis       Date:  1996-02       Impact factor: 4.944

5.  Free radical DNA adduct 8-OH-deoxyguanosine affects activity of Hpa II and Msp I restriction endonucleases.

Authors:  P W Turk; S A Weitzman
Journal:  Free Radic Res       Date:  1995-09

6.  Determination of 8-oxoguanine in DNA by gas chromatography--mass spectrometry and HPLC--electrochemical detection: overestimation of the background level of the oxidized base by the gas chromatography--mass spectrometry assay.

Authors:  J L Ravanat; R J Turesky; E Gremaud; L J Trudel; R H Stadler
Journal:  Chem Res Toxicol       Date:  1995-12       Impact factor: 3.739

Review 7.  Repair of oxidative damage to DNA: enzymology and biology.

Authors:  B Demple; L Harrison
Journal:  Annu Rev Biochem       Date:  1994       Impact factor: 23.643

8.  8-Hydroxydeoxyguanosine. A marker of oxidative DNA damage in systemic lupus erythematosus.

Authors:  J Lunec; K Herbert; S Blount; H R Griffiths; P Emery
Journal:  FEBS Lett       Date:  1994-07-11       Impact factor: 4.124

9.  Synthesis of multiply-labeled [15N3,13C1]-8-oxo-substituted purine bases and their corresponding 2'-deoxynucleosides.

Authors:  R H Stadler; A A Staempfli; L B Fay; R J Turesky; D H Welti
Journal:  Chem Res Toxicol       Date:  1994 Nov-Dec       Impact factor: 3.739

Review 10.  Reactive oxygen species in tumorigenesis.

Authors:  D I Feig; T M Reid; L A Loeb
Journal:  Cancer Res       Date:  1994-04-01       Impact factor: 12.701
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  27 in total

1.  A reliable assessment of 8-oxo-2-deoxyguanosine levels in nuclear and mitochondrial DNA using the sodium iodide method to isolate DNA.

Authors:  M L Hamilton; Z Guo; C D Fuller; H Van Remmen; W F Ward; S N Austad; D A Troyer; I Thompson; A Richardson
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2.  Neurotoxicity of methamphetamine and methylenedioxymethamphetamine.

Authors:  L S Seiden; R Lew; J E Malberg
Journal:  Neurotox Res       Date:  2001-01       Impact factor: 3.911

3.  Measurement of oxidative DNA damage by gas chromatography-mass spectrometry: ethanethiol prevents artifactual generation of oxidized DNA bases.

Authors:  A Jenner; T G England; O I Aruoma; B Halliwell
Journal:  Biochem J       Date:  1998-04-15       Impact factor: 3.857

Review 4.  Formation and repair of oxidatively generated damage in cellular DNA.

Authors:  Jean Cadet; Kelvin J A Davies; Marisa Hg Medeiros; Paolo Di Mascio; J Richard Wagner
Journal:  Free Radic Biol Med       Date:  2017-01-02       Impact factor: 7.376

5.  A novel method for detecting 7-methyl guanine reveals aberrant methylation levels in Huntington disease.

Authors:  Beena Thomas; Samantha Matson; Vanita Chopra; Liping Sun; Swati Sharma; Steven Hersch; H Diana Rosas; Clemens Scherzer; Robert Ferrante; Wayne Matson
Journal:  Anal Biochem       Date:  2013-02-12       Impact factor: 3.365

6.  The protective effects of metyrosine, lacidipine, clonidine, and moxonidine on kidney damage induced by unilateral ureteral obstruction in rats.

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Journal:  Surg Today       Date:  2011-12-28       Impact factor: 2.549

7.  The effect of laparoscopic and open surgery on oxidative DNA damage and IL-37 in children with acute appendicitis.

Authors:  Kemal Ayengin; Hamit Hakan Alp; Veli Avci; Zübeyir Huyut
Journal:  Ir J Med Sci       Date:  2020-07-17       Impact factor: 1.568

8.  Protective effect of resveratrol against methotrexate-induced oxidative stress in the small intestinal tissues of rats.

Authors:  Aynur Arslan; Fatih Ozcicek; Ferda Keskin Cimen; Durdu Altuner; Oguzhan Yarali; Nezahat Kurt; Levent Tumkaya; Cengiz Ozturk; Halis Suleyman
Journal:  Int J Clin Exp Med       Date:  2015-07-15

9.  Investigation of effects of Lacidipine, Ramipril and Valsartan on DNA damage and oxidative stress occurred in acute and chronic periods following isoproterenol-induced myocardial infarct in rats.

Authors:  Mevlut Sait Keles; Yasin Bayir; Halis Suleyman; Zekai Halici
Journal:  Mol Cell Biochem       Date:  2009-03-19       Impact factor: 3.396

10.  Hydrogen peroxide-induced DNA damage is independent of nuclear calcium but dependent on redox-active ions.

Authors:  L Jornot; H Petersen; A F Junod
Journal:  Biochem J       Date:  1998-10-01       Impact factor: 3.857
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